The role of UVA radiation in ketoprofen-mediated BRAF-mutant amelanotic melanoma cells death – A study at the cellular and molecular level

Banach, Klaudia; Kowalska, Justyna; Rzepka, Zuzanna; Beberok, Artur; Rok, Jakub; Wrześniok, Dorota

doi:10.1016/j.tiv.2021.105108

Cited by 9 publications

(12 citation statements)

References 47 publications

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“…Similar effects were demonstrated after treatment with KTP and UVAR on amelanotic melanoma cells of the C32 line and the co-therapy caused an increase in the number of apoptotic cells [ 18 ]. Studies concerning the phototoxic effect of tetracycline and oxytetracycline on melanocytes showed significant cytotoxicity of the tested drugs, when compared with the data from the current study.…”

Section: Discussionsupporting

confidence: 60%

“…Similar conclusions were made in the study assessing the effect of KTP and UVAR treatment on amelanotic melanoma cells. The percentage of cells with depolarized mitochondrial membrane increased from 8 to 50% when C32 cells were exposed to KTP at a concentration of 1.0 mM and UVAR after a 48-h post-incubation time [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…Similar conclusions were made in the study assessing the effect of KTP and UVAR treatment of C32 cell lines. KTP combined with UVAR induced DNA fragmentation, only 48 h after the irradiation procedure [ 18 ]. It was reported that exposure to ciprofloxacin at the concentration of 1.0 mM for 48 h and 72 h caused DNA fragmentation in melanoma cells [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, selectivity was tested in the mode of action of KTP and UVAR, and melanocytes were used as an experimental in vitro model. The obtained results indicate that KTP and UVAR had no effect on GSH levels in melanocytes [ 18 ]. The reason for the difference in GSH levels in melanoma and melanocytes is the different susceptibility of cells to oxidative-reduction imbalance.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the study on the normal cells—melanocytes (HEMn-DP)—was also expanded. This study was carried out as a continuation of the multistage research concerning the affinity of KTP and UVAR for cell proliferation, their cytotoxic and pro-apoptotic properties, and their impact on cell cycle in melanotic melanoma and melanocytes in relation to the anti-tumor effects to KTP and UVAR [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

Banach

Kowalska

Rzepka

et al. 2021

IJMS

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Malignant melanoma is responsible for the majority of skin cancer-related deaths. The methods of cancer treatment include surgical removal, chemotherapy, immunotherapy, and targeted therapy. However, neither of these methods gives satisfactory results. Therefore, the development of new anticancer therapeutic strategies is very important and may extend the life span of people suffering from melanoma. The aim of this study was to examine the effect of ketoprofen (KTP) and UVA radiation (UVAR) therapy on cell proliferation, apoptosis, and cell cycle distribution in both melanotic melanoma cells (COLO829) and human melanocytes (HEMn-DP) in relation to its supportive effect in the treatment of melanoma. The therapy combining the use of pre-incubation with KTP and UVAR causes a significant increase in the anti-proliferative properties of ketoprofen towards melanoma cells and the co-exposure of melanotic melanoma cells induced apoptosis shown as the mitochondrial membrane breakdown, cell-cycle deregulation, and DNA fragmentation. Moreover, co-treatment led to GSH depletion showing its pro-apoptotic effect dependent on ROS overproduction. The treatment did not show a significant effect on normal cells—melanocytes—which indicates its high selectivity. The results suggest a possible benefit from the use of the ketoprofen and ultraviolet A irradiation as a new concept of melanotic melanoma therapy.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

Banach

Kowalska

Rzepka

et al. 2021

IJMS

Self Cite

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A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Vozniuk,

Kejík,

Veselá

et al. 2023

ChemistryOpen

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A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3–100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.

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Integrative lactylation and tumor microenvironment signature as prognostic and therapeutic biomarkers in skin cutaneous melanoma

Zhu,

Song,

Yang

et al. 2023

J Cancer Res Clin Oncol

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The role of UVA radiation in ketoprofen-mediated BRAF-mutant amelanotic melanoma cells death – A study at the cellular and molecular level

Cited by 9 publications

References 47 publications

Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Integrative lactylation and tumor microenvironment signature as prognostic and therapeutic biomarkers in skin cutaneous melanoma

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