The role of uspE in virulence and biofilm formation by Histophilus somni

Pan, Yu; Subhadra, Bindu; Sandal, Indra; Dickerman, Allan W.; Inzana, Thomas J.

doi:10.1016/j.vetmic.2021.109267

Cited by 5 publications

(1 citation statement)

References 55 publications

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“…Our group has reported that UspE is a global regulator controlling virulence and biofilm formation in H . somni [ 64 ]. The LuxR family candidate HS_0901 (regulatory protein, sigma-70 region 4 type 2) and LuxR family candidate HS_0042 (nitrate/nitrite response regulator) are yet to be characterized in H .…”

Section: Discussionmentioning

confidence: 99%

Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336

et al. 2023

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Small RNAs (sRNA), in association with the global chaperone regulator Hfq, positively or negatively regulate gene expression in bacteria. For this study, Histophilus somni sRNAs that bind to Hfq were identified and then partially characterized. The Hfq-associated sRNAs in H. somni were isolated and identified by co-immunoprecipitation using anti-Hfq antibody, followed by sRNA sequencing. Sequence analysis of the sRNA samples identified 100 putative sRNAs, out of which 16 were present in pathogenic strain 2336, but not in non-pathogenic strain 129Pt. Bioinformatic analyses suggested that the sRNAs HS9, HS79, and HS97 could bind to many genes putatively involved in virulence/biofilm formation. Furthermore, multi-sequence alignment of the sRNA regions in the genome revealed that HS9 and HS97 could interact with sigma 54, which is a transcription factor linked to important bacterial traits, including motility, virulence, and biofilm formation. Northern blotting was used to determine the approximate size, abundance and any processing events attributed to the sRNAs. Selected sRNA candidates were confirmed to bind Hfq, as determined by electrophoretic mobility shift assays using sRNAs synthesized by in vitro transcription and recombinant Hfq. The exact transcriptional start site of the sRNA candidates was determined by RNA ligase-mediated rapid amplification of cDNA ends, followed by cloning and sequencing. This is the first investigation of H. somni sRNAs that show they may have important regulatory roles in virulence and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336

et al. 2023

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Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis

Lv,

Shang,

et al. 2023

Microbes and Infection

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Contribution of Hfq to gene regulation and virulence in Histophilus somni

Cao,

Subhadra,

Lee

et al. 2024

Infect Immun

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Histophilus somni is one of the predominant bacterial pathogens responsible for bovine respiratory and systemic diseases in cattle. Despite the identification of numerous H. somni virulence factors, little is known about the regulation of such factors. The post-transcriptional regulatory protein Hfq may play a crucial role in regulation of components that affect bacterial virulence. The contribution of Hfq to H. somni phenotype and virulence was investigated following creation of an hfq deletion mutant of H. somni strain 2336 (designated H. somni 2336Δ hfq ). A comparative analysis of the mutant to the wild-type strain was carried out by examining protein and carbohydrate phenotype, RNA sequence, intracellular survival in bovine monocytes, serum susceptibility, and virulence studies in mouse and calf models. H. somni 2336Δ hfq exhibited a truncated lipooligosaccharide (LOS) structure, with loss of sialylation. The mutant demonstrated increased susceptibility to intracellular and serum-mediated killing compared to the wild-type strain. Transcriptomic analysis displayed significant differential expression of 832 upregulated genes and 809 downregulated genes in H. somni 2336Δ hfq compared to H. somni strain 2336, including significant downregulation of lsgB and licA , which contribute to LOS oligosaccharide synthesis and sialylation. A substantial number of differentially expressed genes were associated with polysaccharide synthesis and other proteins that could influence virulence. The H. somni 2336Δ hfq mutant strain was attenuated in a mouse septicemia model and somewhat attenuated in a calf intrabronchial challenge model. H. somni was recovered less frequently from nasopharyngeal swabs, endotracheal aspirates, and lung tissues of calves challenged with H. somni 2336Δ hfq compared to the wild-type strain, and the percentage of abnormal lung tissue in calves challenged with H. somni 2336Δ hfq was lower than in calves challenged with the wild-type strain. In conclusion, our results support that Hfq accounts for the regulation of H. somni virulence factors.

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The role of uspE in virulence and biofilm formation by Histophilus somni

Cited by 5 publications

References 55 publications

Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336

Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336

Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis

Contribution of Hfq to gene regulation and virulence in Histophilus somni

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