The role of TREM-2 in internalization and intracellular survival of Brucella abortus in murine macrophages

Pan, Wei; Lü, Qiang; Cui, Guimei; Zhang, Guan; Li, Yang; Sun, Changjiang; Sun, Wanchun; Peng, Qisheng

doi:10.1016/j.vetimm.2014.12.007

Cited by 16 publications

(8 citation statements)

References 29 publications

(49 reference statements)

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“…The observed suppression of nitrite production likely facilitates the survival of Brucella in macrophages [ 28 ]. This hypothesis is also supported by our data that transfection of the miR-125b-5p prior to infection is associated with ERK activation, another marker of macrophage activation, which is consistent with the findings in TREM-2 knock-down macrophages [ 2 ]. Our results demonstrate an unappreciated role of miR-125b-5p downregulation in favoring B. abortus intracellular survival.…”

Section: Discussionsupporting

confidence: 91%

“…Brucellae are intracellular pathogens that have the ability to survive and multiply inside professional phagocytic cells. The success of Brucella as an intracellular pathogen depends largely on its ability to avoid the activation of host macrophages upon infection [ 2 , 3 ]. Brucella reprograms the host macrophage transcriptome by suppressing the expression of activation-related genes [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

et al. 2016

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BackgroundBrucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear.ResultsIn this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macrophages. We establish that miR-125b-5p targets A20, an inhibitor of the NF-kB activation. Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus-infected macrophages and leads to NF-kB activation and increased production of TNFα. Furthermore, B. abortus survival is attenuated in the presence of miR-125b-5p.ConclusionsThese results uncover a role for miR-125b-5p in the regulation of B. abortus intracellular survival via the control of A20 expression.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

et al. 2016

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“…Cellular studies have shown that TREM2 regulates the response to bacteria, in two examples by promoting killing of bacteria through production of reactive oxygen species (ROS) [75, 76] – even against bacteria that were not directly detected by TREM2 [75]. The role of TREM2 may vary with different pathogens, as it did not promote killing of B. abortus , [77] a pathogen that specializes in invading and surviving in macrophages.…”

Section: Trem2 In Diseasementioning

confidence: 99%

TREM2-Ligand Interactions in Health and Disease

Kober

Brett

2017

Journal of Molecular Biology

165

160

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The protein Triggering receptor expressed on myeloid cells-2 (TREM2) is an immunomodulatory receptor with a central role in myeloid cell activation and survival. In recent years, the importance of TREM2 has been highlighted by the identification of coding variants that increase risk for Alzheimer’s disease and other neurodegenerative diseases. Animal studies have further shown the importance of TREM2 in neurodegenerative and other inflammatory disease models including chronic obstructive pulmonary disease, multiple sclerosis, and stroke. A mechanistic understanding of TREM2 function remains elusive, however, due in part to the absence of conclusive information regarding the identity of endogenous TREM2 ligands. While many TREM2 ligands have been proposed, their physiological role and mechanism of engagement remain to be determined. In this review, we highlight the suggested roles of TREM2 in these diseases, recent advances in our understanding of TREM2, and discuss putative TREM2-ligand interactions and their potential roles in signaling during health and disease. We develop a model based on the TREM2 structure to explain how different TREM2 ligands might interact with the receptor and how disease-risk variants may alter ligand interactions. Finally, we propose future experimental directions to establish the role and importance of these different interactions on TREM2 function.

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“…As indicated in Figure 4A , the production of ROS in BMDMs infected with Cu-Zn SOD-overexpressing B. abortus is similar to BMDMs infected with wild type strains. As nitric oxygen (NO) plays an important role in killing of the intracellular bacteria [ 13 ], we also investigate whether overexpressed Cu-Zn SOD participate in the production of NO. Consistent with the ROS assay, there was no significant change of NO production in BMDMs infected with wild type or Cu-Zn SOD-overexpressing strains (Figure 4B ), suggesting that overexpression of Cu-Zn SOD does not mediate the NO production.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of Cu-Zn SOD in Brucella abortus suppresses bacterial intracellular replication via down-regulation of Sar1 activity

Liu

Zhou²,

Yang

et al. 2018

Oncotarget

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Brucella Cu-Zn superoxide dismutase (Cu-Zn SOD) is a periplasmic protein, and immunization of mice with recombinant Cu-Zn SOD protein confers protection against Brucella abortus infection. However, the role of Cu-Zn SOD during the process of Brucella infection remains unknown. Here, we report that Cu-Zn SOD is secreted into culture medium and is translocated into host cells independent of type IV secretion systems (T4SS). Furthermore, co-immunoprecipitation and immunofluorescence studies reveal that Brucella abortus Cu-Zn SOD interacts with the small GTPase Sar1. Overexpression of Cu-Zn SOD in Brucella abortus inhibits bacterial intracellular growth by abolishing Sar1 activity in a manner independent of reactive oxygen species (ROS) production.

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The role of TREM-2 in internalization and intracellular survival of Brucella abortus in murine macrophages

Cited by 16 publications

References 29 publications

MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

TREM2-Ligand Interactions in Health and Disease

Overexpression of Cu-Zn SOD in Brucella abortus suppresses bacterial intracellular replication via down-regulation of Sar1 activity

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