The role of transport proteins in the production of microbial glycolipid biosurfactants

Claus, Silke; Sánchez, Liam Richard Jenkins; Bogaert, Inge Van

doi:10.1007/s00253-021-11156-7

Cited by 8 publications

(5 citation statements)

References 124 publications

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“…As the type of carbon source also plays a critical role in the production of surfactant compounds by microorganisms [22], it appears that the absorption of glycerol can help to optimize the production processes of biosurfactant glycolipids, such as the production of sophorolipids (SL) by Starmerella bombicola [23]. Like the present study, glycerol was reported as one of the best carbon sources for glycolipid production by Pseudomonas sp.…”

Section: Production and Characterization Of The Bioemulsifier Produce...supporting

confidence: 53%

Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity

Araujo,

Monteiro,

Silva

et al. 2023

Antibiotics

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Surface-active compounds (SACs) of microbial origin are an active group of biomolecules with potential use in the formulation of emulsions. In this sense, the present study aimed to isolate and select yeasts from fruits that could produce SACs for essential oil emulsions. The Candida krusei M4CK was isolated from the Byrsonima crassifolia fruit to make SACs. This emulsification activity (E24) was equal to or greater 50% in all carbon sources, such as olive oil, sunflower oil, kerosene, hexane, and hexadecane. E24 followed exponential growth according to the growth phase. The stability of emulsions was maintained over a wide range of temperatures, pH, and salinity. The OMBE4CK (melaleuca essential oil emulsion) had better and more significant inhibitory potential for biofilm reduction formation. In addition, bioemulsifier BE4CK alone on Escherichia coli and Pseudomonas aeruginosa biofilm showed few effective results, while there was a significant eradication for Staphylococcus aureus biofilms. The biofilms formed by S. aureus were eradicated in all concentrations of OMBE4CK. At the same time, the preformed biofilm by E. coli and P. aeruginosa were removed entirely at concentrations of 25 mg/mL, 12.5 mg/mL, and 6.25 mg/mL. The results show that the bioemulsifier BE4CK may represent a new potential for antibiofilm application.

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Section: Production and Characterization Of The Bioemulsifier Produce...supporting

confidence: 53%

Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity

Araujo,

Monteiro,

Silva

et al. 2023

Antibiotics

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“…Furthermore, several ABC-transporters associated with secondary metabolism are highly conserved among fungi [23]. All fungal glycolipid biosurfactants produced by yeasts that are characterized so far are also organized in such clustered architecture with a transporter in its center, as was recently reviewed by our group [55]. Taken together, it is not surprising that SLMdr.1 is under heavy transcriptional regulations that coincide with the rest of the cluster genes.…”

Section: Slmdr2: the Second Sl Transportermentioning

confidence: 80%

Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola

Claus

Jezierska

Elbourne

et al. 2021

Preprint

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Starmerella bombicola is a non-conventional yeast mainly known for its capacity to produce high amounts of the glycolipids ‘sophorolipids’. Although its product has been used as biological detergent for a couple of decades, the genetics of S. bombicola are still largely unknown. Computational analysis of the yeast’s genome enabled us to identify 254 putative transporter genes that make up the entire transportome. For each of them, a potential substrate was predicted using homology analysis, subcellular localization prediction and RNA sequencing in different stages of growth. One transporter family is of exceptional importance to this yeast: the ATP Binding Cassette (ABC) transporter Superfamily, because it harbors the main driver behind the highly efficient sophorolipid export. Furthermore, members of this superfamily translocate a variety of compounds ranging from antibiotics to hydrophobic molecules. We conducted an analysis of this family by creating deletion mutants to understand their role in the export of hydrophobic compounds, antibiotics and sophorolipids. Doing this, we could experimentally confirm the transporters participating in the efflux of medium chain fatty alcohols, particularly decanol and undecanol, and identify a second sophorolipid transporter that is located outside the sophorolipid biosynthetic gene cluster.

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“…Those references rehearse the fact that even tiny molecules like water [ 510 , 511 ] do not pass unhindered through phospholipid bilayers in real biological membranes (whose protein : lipid ratio by mass is often 3 : 1), but require transporters. Recent examples of transporter engineering for biotechnological purposes include glycolipid surfactants [ 512 ] and fatty acids [ 513 ]. Flow cytometry can provide a convenient means of assessing the activities of certain transporters [ 179 , 180 ].…”

Section: Membrane Transporter Engineeringmentioning

confidence: 99%

Intelligent host engineering for metabolic flux optimisation in biotechnology

Munro

Kell

2021

Biochemical Journal

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Optimising the function of a protein of length N amino acids by directed evolution involves navigating a ‘search space’ of possible sequences of some 20N. Optimising the expression levels of P proteins that materially affect host performance, each of which might also take 20 (logarithmically spaced) values, implies a similar search space of 20P. In this combinatorial sense, then, the problems of directed protein evolution and of host engineering are broadly equivalent. In practice, however, they have different means for avoiding the inevitable difficulties of implementation. The spare capacity exhibited in metabolic networks implies that host engineering may admit substantial increases in flux to targets of interest. Thus, we rehearse the relevant issues for those wishing to understand and exploit those modern genome-wide host engineering tools and thinking that have been designed and developed to optimise fluxes towards desirable products in biotechnological processes, with a focus on microbial systems. The aim throughput is ‘making such biology predictable’. Strategies have been aimed at both transcription and translation, especially for regulatory processes that can affect multiple targets. However, because there is a limit on how much protein a cell can produce, increasing kcat in selected targets may be a better strategy than increasing protein expression levels for optimal host engineering.

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The role of transport proteins in the production of microbial glycolipid biosurfactants

Cited by 8 publications

References 124 publications

Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity

Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity

Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola

Intelligent host engineering for metabolic flux optimisation in biotechnology

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