The role of transglutaminases in the regulation of phosphatidylserine-positive platelet formation

Kotova, Ya. N.; Абаева, А. А.; Kolyadko, Vladimir N.; Yakimenko, Alena O.; Ataullakhanov, F. I.; Panteleev, Mikhail A.

doi:10.1134/s1990747815040054

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…9 In addition, platelet fXIIIa may contribute to thrombosis through the formation of procoagulant platelets. 10,11 Together with platelet-derived tissue transglutaminase (tTG), fXIIIa may participate in the retention of α-granule proteins in the 'cap'-like structures 12,13 of the phosphatidylserine (PS)-positive (procoagulant) platelet subpopulation, 14,15 thus contributing to the formation of a procoagulant surface.…”

Section: Introductionmentioning

confidence: 99%

Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen

et al. 2019

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Factor XIIIa (fXIIIa) is a transglutaminase that plays a crucial role in fibrin clot stabilization and regulation of fibrinolysis. It is known to bind to procoagulant platelets. In contrast, the zymogen fXIII interaction with platelets is not well characterized. We investigated the interaction of zymogen fXIII with activated platelet subpopulations. Confocal microscopy and flow cytometry using fluorescently labelled factors and antibodies. Phosphatidylserine (PS)-positive activated platelets bound 700 to 800 molecules/cell of fXIII at 100 nM, while both PS-negative activated platelets and resting platelets bound 200 to 400 molecules/cell. The binding was reversible, calcium-independent and linear within the fXIII concentration range of up to 1,000 nM. fXIII predominantly bound to the caps of procoagulant platelets and co-localized with fibrinogen. Exogenous fibrinogen promoted fXIII binding by activated PS-negative platelets; this effect was abolished by the integrin αIIbβ3 antagonist monafram. The fXIII binding was 1.5- to 3-fold decreased for platelets from four patients with grey platelet syndrome, and was variable for platelets from six patients with Glanzmann's thrombasthenia. Strong platelet stimulation, fibrinogen and αIIbβ3 play essential roles in fXIII binding, without any of them fXIII does not bind to platelets. The preferential binding in the cap-like structures might be important for increasing local fXIII concentration in platelet thrombi.

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Section: Introductionmentioning

confidence: 99%

Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen

et al. 2019

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“…This enzyme only promotes proteins by accelerating the covalent bond between the carboxylic amide group of the lateral chain of the glutamate root and the lysine side chain amino acid group. 1 Wu and Cork used the enzyme in the White Noodle Building in 2005, 2 and stated that the samples had better rheological properties than the enzyme-free control sample, slightly less desirable color, less solids lost to cooking water. In 2016, Comino et al 3 reported that adding some rye flour to wheat flour could produce more water-absorbing paste that also preserves its novelty from the dough.…”

Section: Introductionmentioning

confidence: 99%

The study of nutrition value and physico-chemical properties on cross- linked rye noodles

Shahsavani¹

2018

JNHFE

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Each noodle sample was evaluated for moisture, crude protein, crude fat, crude fiber, and ash contents according to (AOAC 2003) the Official methods of analysis of the Association of Official Analytical Chemists. 7 All measurements were expressed as on a dry weight basis and performed in triplicate. Cooking properties Cooking yield and cooking loss of the noodles were determined as described by Akanbi & Foo et al. 8,9 precisely 30 g of the noodles

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The role of transglutaminases in the regulation of phosphatidylserine-positive platelet formation

Cited by 2 publications

References 15 publications

Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen

Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen

The study of nutrition value and physico-chemical properties on cross- linked rye noodles

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