The role of transacylases in the metabolism of arachidonate and platelet activating factor

Snyder, Fred; Lee, Ten-ching; Blank, Merle L.

doi:10.1016/0163-7827(92)90016-c

Cited by 83 publications

(41 citation statements)

References 153 publications

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“…Acyltransferases catalyze the acylation of lysophospholipids and are involved in the biosynthesis and fatty acid remodeling of phospholipids (1)(2)(3)(4)(5)(6). In mammalian cells and tissues, four types of acylation reactions for lysophospholipids have been proposed, based on the acyl donor and requirement for cofactors, such as CoA.…”

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confidence: 99%

“…The CoA-independent transacylation system is involved in the acylation of 1-Oalkyl-GPC (lysoplatelet-activating factor). Lysophospholipase catalyzes the hydrolysis of the carboxyl ester bonds of lysophospholipids (5,6). Some lysophospholipase isoforms are known to catalyze a transacylation reaction between two LPC molecules, leading to the formation of a diacylphospholipid PC and GPC.…”

mentioning

confidence: 99%

“…These comprise: 1) acyl-CoA:lysophospholipid acyltransferase, 2) the CoA-dependent transacylation system, 3) the CoA-independent transacylation system and 4) lysophospholipase/transacylase. Acyl-CoA:lysophospholipid acyltransferases utilize acyl-CoA as an acyl donor and various lysophospholipids as acyl acceptors (1)(2)(3)(4)(5)(6)(7)(8). Lands et al (1,7,8) proposed that the acyl-CoA: lysophospholipid acyltransferases are involved in the accumulation of polyunsaturated fatty acids in phospholipids because they preferentially utilize polyunsaturated fatty acid acyl-CoA as an acyl donor when 1-acyl-glycerophosphocholine (1-acyl-GPC 1 ; lysophosphatidylcholine, LPC) or 1-acyl-glycerophosphoinositol (1-acyl-GPI; lysophosphatidylinositol, LPI) serves as the acyl acceptor.…”

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confidence: 99%

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ATP-independent Fatty Acyl-Coenzyme A Synthesis from Phospholipid

Yamashita¹,

Kawagishi²,

Miyashita³

et al. 2001

Journal of Biological Chemistry

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CoA-dependent transacylation activity in microsomes is known to catalyze the transfer of fatty acids between phospholipids and lysophospholipids in the presence of CoA without the generation of free fatty acids. We previously found a novel acyl-CoA synthetic pathway, ATP-independent acyl-CoA synthesis from phospholipids. We proposed that: 1) the ATP-independent acyl-CoA synthesis is due to the reverse reaction of acyl-CoA:lysophospholipid acyltransferases and 2) the reverse and forward reactions of acyltransferases can combine to form a CoA-dependent transacylation system. To test these proposals, we examined whether or not recombinant mouse acyl-CoA:1-acyl-sn-glycero-3-phosphate (lysophosphatidic acid, LPA) acyltransferase (LPAAT) could catalyze ATPindependent acyl-CoA synthetic activity and CoA-dependent transacylation activity. ATP-independent acyl-CoA synthesis was indeed found in the membrane fraction from Escherichia coli cells expressing mouse LPAAT, whereas negligible activity was observed in mock-transfected cells. Phosphatidic acid (PA), but not free fatty acids, served as an acyl donor for the reaction, and LPA was formed from PA in a CoA-dependent manner during acyl-CoA synthesis. These results indicate that the ATP-independent acyl-CoA synthesis was due to the reverse reaction of LPAAT. In addition, bacterial membranes containing LPAAT catalyzed CoA-dependent acylation of LPA; PA but not free fatty acid served as an acyl donor. These results indicate that the CoA-dependent transacylation of LPA consists of 1) acyl-CoA synthesis from PA through the reverse action of LPAAT and 2) the transfer of the fatty acyl moiety of the newly formed acyl-CoA to LPA through the forward reaction of LPAAT.

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confidence: 99%

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confidence: 99%

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ATP-independent Fatty Acyl-Coenzyme A Synthesis from Phospholipid

Yamashita¹,

Kawagishi²,

Miyashita³

et al. 2001

Journal of Biological Chemistry

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“…Phospholipids have a structural role in red blood cell membrane meanwhile they have a role in phospholipids in plasma. Different types of fatty acids are distributed in various kinds of phospholipids (1,2). The fatty acyl residues of individual phospholipids are under strict metabolic regulation.…”

Section: Introductionmentioning

confidence: 99%

Phospholipid Fatty Acid Composition of Rat, Rabbit, Human and Dog Plasma

Pekiner¹

2003

Ankara Universitesi Eczacilik Fakultesi Dergisi

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“…Different kinds of fatty acids are distributed in phospholipids in various animal tissues (5)(6)(7)(8)(9)(10)(11)(12)(13). In red blood cell (RBC) membrane, phospholipids have a structural role whilst in plasma phospholipids play a role in lipoproteins.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Composition of Red Blood Cell Membrane Phosphatidylethanolamine and Phosphatidylcholine in Rat, Rabbit, Human and Dog : Siçan, Tavşan, İnsan Ve Köpek Eri̇trosi̇t Membrani Fosfati̇di̇letanol

Pekiner¹

2002

Ankara Universitesi Eczacilik Fakultesi Dergisi

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RBC (red blood cell) membrane fatty acid percentages in PE (phosphatidylethanolamine) and PC (phosphatidylcholine) in different species like rats, rabbits, humans and dogs were measured andcompared. The major fatty acids of membrane PE and PC in the species were same as C 16:0 , C 18:1 ,, C 18:1 , C 18:2 and C 20:4 . SFAs (Saturatedfatty acids) (C 16:0 , C 18:0 , and C 20:0 ) were lower in PE but higher in PC in all species in RBC membrane. Arachidonate (C 20:4 ) and PUFAs (polyunsaturated fatty acid) (C 18:2 , C 18:3 and C 20:4

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The role of transacylases in the metabolism of arachidonate and platelet activating factor

Cited by 83 publications

References 153 publications

ATP-independent Fatty Acyl-Coenzyme A Synthesis from Phospholipid

ATP-independent Fatty Acyl-Coenzyme A Synthesis from Phospholipid

Phospholipid Fatty Acid Composition of Rat, Rabbit, Human and Dog Plasma

Fatty Acid Composition of Red Blood Cell Membrane Phosphatidylethanolamine and Phosphatidylcholine in Rat, Rabbit, Human and Dog : Siçan, Tavşan, İnsan Ve Köpek Eri̇trosi̇t Membrani Fosfati̇di̇letanol

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