The role of TNF-α and TLR4 polymorphisms in the placenta of pregnant women complicated by preeclampsia and in silico analysis

Mohammadpour‐Gharehbagh, Abbas; Jahantigh, Danial; Eskandari, Moein; Eskandari, Fatemeh; Rezaei, Mahnaz; Zeynali-Moghaddam, Shima; Teimoori, Batool; Salimi, Saeedeh

doi:10.1016/j.ijbiomac.2019.05.040

Cited by 18 publications

(15 citation statements)

References 38 publications

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“…However, a meta-analysis indicated that there was no evidence of a difference in the circulating levels of IL-17 between patients with PE and controls . Additionally, several studies have demonstrated that TNF may be associated with PE and TNF-α may be a potential biomarker for PE. ,, More studies are required to explore the roles of the IL-17 and TNF signaling pathways in the development of PE. Additionally, other pathways, including MAPK and toll-like receptor signaling pathways, were significant after changing the background to 92 inflammation-related proteins.…”

Section: Discussionmentioning

confidence: 99%

Plasma Olink Proteomics Identifies CCL20 as a Novel Predictive and Diagnostic Inflammatory Marker for Preeclampsia

Wang

Yip

et al. 2022

J. Proteome Res.

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Inflammation is generally thought to be involved in the occurrence and development of preeclampsia (PE), but its specific effect on PE remains unclear. In the present study, the expression levels of 92 inflammation-related proteins were measured in the late pregnancy maternal plasma from patients with PE (n = 15) and normal pregnant controls (n = 15) using the Olink inflammation panel based on the highly sensitive and specific proximity extension assay technology. A total of 28 inflammation-related markers differed between the PE and control groups. Among them, fibroblast growth factor 21 (FGF-21) and cysteine–cysteine motif chemokine ligand 20 (CCL20) had the largest fold changes. We further validated the levels of CCL20 in the late (43 with PE and 44 controls) and early (37 with PE and 37 controls) pregnancy maternal plasma using enzyme-linked immunosorbent assay (ELISA). To the best of our knowledge, for the first time, CCL20 was found to be upregulated in the late and early pregnancy plasma of patients with PE and had an area under the curve (AUC) of 0.753 and 0.668, respectively. In conclusion, patients with PE had increased levels of most inflammatory markers, and CCL20 might be a novel potential predictive and diagnostic biomarker for PE.

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Section: Discussionmentioning

confidence: 99%

Plasma Olink Proteomics Identifies CCL20 as a Novel Predictive and Diagnostic Inflammatory Marker for Preeclampsia

Wang

Yip

et al. 2022

J. Proteome Res.

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“…Interestingly, the TNF-α -308G/A genotype was remarkably higher in controls relative to PE women, and this genotype could be a protective factor for PE susceptibility. This study for the first time showed evidence of an association between placental TNF-α -308G/A genotype and TNF-α -238A allele with a reduced risk for PE (38). Zubor et al examined the association between G and A alleles of -380G/A polymorphism with other symptoms of PE, which included renal failure, capillary permeability, involvement of other organs, and PE severity.…”

Section: Discussionmentioning

confidence: 94%

The TNF-α -308G/A Gene Polymorphism and Serum TNF-α Levels in Women with Preeclampsia

Khodadadi¹,

Ghadiri²,

Ghafourian³

et al. 2022

JFRH

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Objective: Worldwide, preeclampsia (PE) is a multifactorial disorder reported in 2–5% of pregnancies, which increases mortality during pregnancy. In general, 10–15% of maternal deaths are directly related to PE and eclampsia. One of the susceptibility genes for PE is tumor necrosis factor-α (TNF-α) expressed by most immune cells. TNF-α is a protein involved in various biological processes, including proliferation and apoptosis, as well as the expression of inflammatory genes. The goal of this study was to investigate the role of TNF-α single nucleotide polymorphism (SNP) -308G/A (rs1800629) and their relationship with TNF-α in PE patients. Materials and methods: The SNP was genotyped in 90 cases and 90 controls. Whole blood was collected from women with PE and normal pregnancy in EDTA containing tubes, and DNA extraction was performed from their blood lymphocytes according to a standard phenol-chloroform procedure. Then, DNA was genotyped by real-time PCR and the polymorphism was detected by TaqMan assay. Serum levels of TNF-α protein were measured by enzyme-linked immunosorbent (ELISA) assay. Results: TNF-α levels in women with PE were significantly higher than in healthy ones (p<0.001). We did not observe any correlation between allelic outbreak (p=0.3) and TNF-α-308G/A polymorphism (p=0.7) with the incidence of PE. Conclusion: Although TNF-α-308G/A gene polymorphism does not appear to affect susceptibility to PE, an increased level of serum TNF-α can be used as a predictor for PE during pregnancy. We recommend that more research be conducted on possible factors related to the incidence of PE.

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“…A meta‐analysis of 22 case‐control studies clearly showed that the allele of TNF‐α /rs1800629 A was associated with PE risk in Caucasian and Iranian populations 57 . A recent study demonstrated that the rs1800629 GA genotype and rs361525 A allele can reduce the risk of PE in an Iranian population 59 …”

Section: Genetic Polymorphisms Of Pihmentioning

confidence: 99%

The genetic risk factors for pregnancy‐induced hypertension: Evidence from genetic polymorphisms

et al. 2022

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Pregnancy‐induced hypertension (PIH) is a multifactorial and severe pregnancy complication including preeclampsia/eclampsia, gestational hypertension, chronic (pre‐existing) hypertension, and preeclampsia/eclampsia variants superimposed on chronic hypertension. PIH‐induced maternal mortality accounts for approximately 9% of all maternal deaths over the world. A large number of case‐control studies have established the importance of various genetic factors in the occurrence and development of PIH. In this narrative review, we summarized the genetic risk factors involved in the renin‐angiotensin system, endothelin system, inflammatory factors, oxidative stress, and other functional networks, with the aim of sorting out the genetic factors that may play a potential role in PIH and providing new ideas to elucidate the pathogenesis of PIH.

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The role of TNF-α and TLR4 polymorphisms in the placenta of pregnant women complicated by preeclampsia and in silico analysis

Cited by 18 publications

References 38 publications

Plasma Olink Proteomics Identifies CCL20 as a Novel Predictive and Diagnostic Inflammatory Marker for Preeclampsia

Plasma Olink Proteomics Identifies CCL20 as a Novel Predictive and Diagnostic Inflammatory Marker for Preeclampsia

The TNF-α -308G/A Gene Polymorphism and Serum TNF-α Levels in Women with Preeclampsia

The genetic risk factors for pregnancy‐induced hypertension: Evidence from genetic polymorphisms

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