The Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses

Nandety, Raja Sekhar; Caplan, Jeffery L.; Cavanaugh, Keri; Perroud, Bertrand; Wróblewski, Tadeusz; Michelmore, Richard W.; Meyers, Blake C.

doi:10.1104/pp.113.219162

Cited by 142 publications

(195 citation statements)

References 41 publications

(73 reference statements)

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“…2 F and G and 3 C and D). The ectopic cell-death phenotypes driven by overexpression of TIR-NLR and/or derived single-domain TIRs typically are dependent on the Enhanced disease susceptibility1 (EDS1) signaling module (17,28). We found that RBA1-mediated cell death was also EDS1 dependent in the context of either transient overexpression (in eds1-silenced N. tabacum) or when expressed from its endogenous locus in an Arabidopsis eds1 mutant background ( Fig.…”

Section: Rba1 Is Autoactive and Enhanced Disease Susceptibility 1-depmentioning

confidence: 79%

“…Although RBA1 appears to be a pseudogene in the Col-0 genome, most of the TIR-X and TIR-NB genes are expressed (50). Interestingly, several of these are transcriptionally induced by the defense hormone salicylic acid, suggesting a role in disease resistance (28). Like RBA1 or TIR domains truncated from full-length TNLs, overexpression of TIR-X and TIR-NB proteins can trigger EDS1-dependent cell death (28).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, several of these are transcriptionally induced by the defense hormone salicylic acid, suggesting a role in disease resistance (28). Like RBA1 or TIR domains truncated from full-length TNLs, overexpression of TIR-X and TIR-NB proteins can trigger EDS1-dependent cell death (28). Loss-of-function mutations in TN2, a TIR-NB protein lacking LRRs, were isolated as genetic suppressors of cell death triggered by loss-of-function alleles of EXO70B1, a component of the exocyst vesicle trafficking complex (51).…”

Section: Discussionmentioning

confidence: 99%

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TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

Nishimura

Anderson

Cherkis

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

131

117

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Detection of pathogens by plants is mediated by intracellular nucleotide-binding site leucine-rich repeat (NLR) receptor proteins. NLR proteins are defined by their stereotypical multidomain structure: an N-terminal Toll-interleukin receptor (TIR) or coiled-coil (CC) domain, a central nucleotide-binding (NB) domain, and a C-terminal leucine-rich repeat (LRR). The plant innate immune system contains a limited NLR repertoire that functions to recognize all potential pathogens. We isolated Response to the bacterial type III effector protein HopBA1 (RBA1), a gene that encodes a TIR-only protein lacking all other canonical NLR domains. RBA1 is sufficient to trigger cell death in response to HopBA1. We generated a crystal structure for HopBA1 and found that it has similarity to a class of proteins that includes esterases, the hemebinding protein ChaN, and an uncharacterized domain of Pasteurella multocida toxin. Self-association, coimmunoprecipitation with HopBA1, and function of RBA1 require two previously identified TIR-TIR dimerization interfaces. Although previously described as distinct in other TIR proteins, in RBA1 neither of these interfaces is sufficient when the other is disrupted. These data suggest that oligomerization of RBA1 is required for function. Our identification of RBA1 demonstrates that "truncated" NLRs can function as pathogen sensors, expanding our understanding of both receptor architecture and the mechanism of activation in the plant immune system. plant immunity | NLR | Toll-interleukin-1 receptor homology domain | oligomerization | type III secretion

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Section: Rba1 Is Autoactive and Enhanced Disease Susceptibility 1-depmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

Nishimura

Anderson

Cherkis

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

131

117

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“…Many of the differentially expressed genes were allocated in functional groups related to plant defence processes, such as "response to chitin" or "innate immune defence". In particular, we observed down regulation of a substantial number of genes that codify for TOLL/INTERLEUKIN RECEPTOR (TIR) domain--containing proteins that play an important role in plant defence (Nandety et al 2013). Moreover, we observed the down regulation of several genes involved in cell death (c.f.…”

Section: The Expression Of Ami1 Impacts Several Different Functional mentioning

confidence: 99%

Dissecting the role of Arabidopsis thaliana AMIDASE 1 in auxin biosynthesis

Parra¹

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“…The majority of TIR genes were found residing in a cluster mixed with TNL and TN suggesting they might have derived from TNs or TNLs and co-evolved . Thus, TIR genes could interact and function together with TNL or NL proteins for their involvement in a disease resistance response (Nandety et al, 2013 (Meyers et al, 2003). …”

Section: Genome Wide Identification Of B Napus Nbs-lrr Genesmentioning

confidence: 99%

Genome wide identification of NBS-LRR genes in Brassica and their association with disease resistance in Brassica napus

Alamery¹

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Brassica napus (canola/rapeseed/oilseed rape) is an important commercial oilseed crop in Australia with an annual production of approximately 1.6 million tons of oil. Canola is an important source of edible vegetable oil and has a broad range of industrial purposes. Blackleg disease (stem canker) caused by the fungal pathogen Leptosphaeria maculans, is one of the most devastating diseases in B. napus. This disease causes significant yield losses with an annual average loss of 15 to 48 %, although losses can reach up to 80% worldwide, mainly in Europe, Australia and Canada.In order to develop an effective strategy to control the blackleg disease, there is a need to identify blackleg resistance genes in Brassica species and understand the genetic interaction between plant resistance genes and the pathogen avirulence genes. Thus, identification of Nucleotide Binding SiteLeucine Rich Repeat (NBS-LRR) resistance genes is one of the most important objectives of understanding resistance. NBS-LRR resistance genes have been extensively studied because they represent the largest class of disease resistance genes and play a critical role in defending plants from pathogens.The objective of this study was to perform comprehensive analysis on identification and characterization of NBS-LRR genes in the B. napus genome and to study the synteny and conservation of NBS-LRR genes between Brassica species. In this study, a total of 641, 249 and 443 NBS-LRR encoding genes in B. napus, B. rapa and B. oleracea, respectively, were identified.The comparative analysis between B. napus and its progenitor species indicated that NBS-LRR genes exhibited similar gene structure, genomic location, arrangement in clusters and syntenic relationships. The results provide evidence that there was a selective advantage to maintaining similar features of NBS-LRR genes in B. napus to both B. rapa and B. oleracea following polyploidization. More than 60% of NBS LRR genes from the progenitor species were conserved.The differences in NBS-LRR gene conservation could be attributed to gene losses or selection pressure to offer species-specific or cultivars-specific resistance.This study found that the NBS-LRR resistance genes are physically clustered and individual genes involved in clusters were more polymorphic and subject to evolutionary process than singleton genes. These clusters, which have been described in many other species, provide a reservoir of genetic variation influenced by tandem duplication and selection pressure. In addition, there was a significant correlation and co-localization between the number of NBS-LRR genes within the disease QTL intervals and the number of genes involved in gene clusters or duplication. This ii correlation provides evidence that NBS-LRR are distributed and clustered throughout the genome and tends to be linked and associated with disease QTL intervals. Chalhoub, B., Denoeud, F., Liu, S., Parkin, I. A. P., Tang, H., Wang, X., Chiquet, J., Belcram, H., Tong, C., Samans, B., Corréa, M., Da Silva, C., Just, ...

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The Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses

Cited by 142 publications

References 41 publications

TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

Dissecting the role of Arabidopsis thaliana AMIDASE 1 in auxin biosynthesis

Genome wide identification of NBS-LRR genes in Brassica and their association with disease resistance in Brassica napus

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