The Role of Thoracic Epidural Analgesia in Receptor-Dependent and Receptor-Independent Pulmonary Vasoconstriction in Experimental Pancreatitis

Abstract

Our study demonstrated that TEA improves pancreatitis-associated impairment of pulmonary vasoreactivity and gas exchange.

Gadolinium chloride modulates bradykinin-induced pulmonary vasoconstriction and hypoxic pulmonary vasoconstriction during polymicrobial abdominal sepsis in rats

“…The effects of gadolinium chloride on HPV Consistent with previous studies, we observed that a robust HPV response in isolated and perfused lungs of healthy rats in response to the induction of alveolar hypoxia was blunted in septic rats 24 hours postinjury, indicating a profound dysregulation of HPV in this setting [10,20].…”

“…Prior to the isolation and perfusion of the lungs, the exhaled air from the rats' lungs of all four groups (n = 10 each) was collected in a bag for 10 minute. The exNO concentration was analyzed by a TE 42S NO x analyzer (Thermo Environment, Franklin, Mass, USA) using an established protocol [20,21].…”

“…The circle was closed by a cannula (3.5-mm outside diameter) that was inserted through the apex of the left ventricle and secured with umbilical tape. The isolation and perfusion procedure strictly followed an established and standardized procedure [20,22].…”

“…In this study, the potent receptormediated vasoconstrictor angiotensin II (Ang II, 0.1 μg/0.2 mL, Sigma Chemicals, Germany) was injected into the inflow tract of the isolated perfused lung circuit to test possible functional impairment of VSMC caused by sepsis. Changes in the peak pulmonary artery pressure (peak P a in mmHg) to baseline P a (mmHg) were measured and expressed as pressure difference ( p) from baseline P a [10,20,22]. Pulmonary artery pressure returned to baseline pressures several minutes after exposure to angiotensin II and remained stable, indicating no further vasoactive concentrations circulating in the circuit.…”

Potential of sivelestat in protection against severe acute pancreatitis-associated lung injury in rats

“…Respectively at 6, 12, and 24 hours after the saline or sivelestat administration, six rats from each of the groups were sacrificed. The sample size of 6 was determined based on the differential levels of myeloperoxidase (MPO) in the pulmonary tissue (one of the major measures also used in the present study) in rats in the control and acute pancreatitis conditions reported in a previous study [17] using free online power-sample size calculation software (http://www.stat.ubc.ca/∼rollin/stats/ssize/n1.html). This sample size would ensure an anticipated analysis power of 0.9 for two-sided tests at α = 0.05.…”