The role of thioredoxin h in protein metabolism during wheat (Triticum aestivum L.) seed germination

Guo, Haipeng; Wang, Shaoxin; Xu, Fangsen; Li, Yongchun; Ren, Jie; Xiang, Wang; Niu, Hongbin; Yin, Jing

doi:10.1016/j.plaphy.2013.03.006

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…This QTN was predicted in three different environments i.e., E 1 , E 3, and E 4, and using three different models, suggesting this could be a reliable QTN contributing to GL variation in wheat. In an earlier study, Thioredoxin has been shown to play an important role in preventing sprouting of developing grains in cereals (wheat and barley) by reducing the intramolecular disulfide bonds of storage proteins and other proteins in the starchy endosperm, and thereby affecting grain yield (Guo et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Q.TGW-1A (SNP-AX-94605845) was annotated as TTL1 protein (TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE 1) with R 2 = 11.78% and highly expressed in large grains. Studies have reported that TTL1 positively regulates the stress response regulated by ABA (Guo et al, 2013). The loss of TTL1 function causes plants to be sensitive to salt and osmotic stress during seed germination and later development (Rosado et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Association mapping reveals novel genes and genomic regions controlling grain size architecture in mini core accessions of Indian National Genebank wheat germplasm collection

et al. 2023

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Wheat (Triticum aestivum L.) is a staple food crop for the global human population, and thus wheat breeders are consistently working to enhance its yield worldwide. In this study, we utilized a sub-set of Indian wheat mini core germplasm to underpin the genetic architecture for seed shape-associated traits. The wheat mini core subset (125 accessions) was genotyped using 35K SNP array and evaluated for grain shape traits such as grain length (GL), grain width (GW), grain length, width ratio (GLWR), and thousand grain weight (TGW) across the seven different environments (E1, E2, E3, E4, E5, E5, E6, and E7). Marker-trait associations were determined using a multi-locus random-SNP-effect Mixed Linear Model (mrMLM) program. A total of 160 non-redundant quantitative trait nucleotides (QTNs) were identified for four grain shape traits using two or more GWAS models. Among these 160 QTNs, 27, 36, 38, and 35 QTNs were associated for GL, GW, GLWR, and TGW respectively while 24 QTNs were associated with more than one trait. Of these 160 QTNs, 73 were detected in two or more environments and were considered reliable QTLs for the respective traits. A total of 135 associated QTNs were annotated and located within the genes, including ABC transporter, Cytochrome450, Thioredoxin_M-type, and hypothetical proteins. Furthermore, the expression pattern of annotated QTNs demonstrated that only 122 were differentially expressed, suggesting these could potentially be related to seed development. The genomic regions/candidate genes for grain size traits identified in the present study represent valuable genomic resources that can potentially be utilized in the markers-assisted breeding programs to develop high-yielding varieties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association mapping reveals novel genes and genomic regions controlling grain size architecture in mini core accessions of Indian National Genebank wheat germplasm collection

et al. 2023

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“…Wheat lines with underexpressed Trx showed delayed preharvest sprouting when grown in the greenhouse or in a field without a decrease in final yield ( Li et al., 2009 ). The expression of protein disulfide isomerase in the thioredoxin s antisense transgenic wheat was up-regulated, which induced easily forming glutenin macropolymers and the resistance of storage proteins to degradation ( Guo et al., 2013 ). The results are further evidence that the level of Trx H in cereal endosperm determines the fundamental properties as well as the potential applications of the seed.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, characterization, evolution, and expression pattern analyses of the typical thioredoxin gene family in wheat (Triticum aestivum L.)

et al. 2022

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Typical thioredoxin (TRX) plays an important role in maintaining redox balance in plants. However, the typical TRX genes in wheat still need to be comprehensively and deeply studied. In this research, a total of 48 typical TaTRX genes belonging to eight subtypes were identified via a genome-wide search in wheat, and the gene structures, protein conserved motifs, and protein 3D structures of the same subtype were very similar. Evolutionary analysis showed that there are two pairs of tandem duplication genes and 14 clusters of segmental duplication genes in typical TaTRX family members; TaTRX15, TaTRX36, and TaTRX42 had positive selection compared with the orthologs of their ancestral species; rice and maize have 11 and 13 orthologous typical TRXs with wheat, respectively. Gene Ontology (GO) analysis indicated that typical TaTRXs were involved in maintaining redox homeostasis in wheat cells. Estimation of ROS content, determination of antioxidant enzyme activity, and gene expression analysis in a line overexpressing one typical TaTRX confirmed that TRX plays an important role in maintaining redox balance in wheat. A predictive analysis of cis-acting elements in the promoter region showed that typical TaTRXs were extensively involved in various hormone metabolism and response processes to stress. The results predicted using public databases or verified using RT-qPCR show that typical TaTRXs were able to respond to biotic and abiotic stresses, and their expression in wheat was spatiotemporal. A total of 16 wheat proteins belonging to four different families interacting with typical TaTRXs were predicted. The above comprehensive analysis of typical TaTRX genes can enrich our understanding of this gene family in wheat and provide valuable insights for further gene function research.

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“…This role is proved by the identification of a large number of proteins as Trxs targets in cereals grain [16,18]. Down-regulation of wheat Trxh induced easily forming glutenin macropolymers and the resistance of storage proteins to degradation [19]. Trxs h are involved in a complex interplay with other redox regulators such as glutaredoxin (Grx), glutathione (GSH) or peroxiredoxins (Prxs), also implicated against the oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Defense Responses of Wheat Seedling under Biotic Stress Mimicked by the Linear β-(1,3)-Glucan

Zahid¹

2020

GJSFR

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Reactive oxygen species (ROS) production causes damage, and to better deal with the toxic effects of ROS, the seeds have developed detoxification mechanisms, among which the enzymes of the antioxidant system (catalase, superoxide dismutase, ascorbate peroxidase). Another result supports the link between ROS and redox regulation catalyzed by redoxin family in the seed. Among which, thioredoxins (Trxs) and peroxiredoxins (Prxs), particularly 1-Cys-Prx, expressed during maturation and germination steps.

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The role of thioredoxin h in protein metabolism during wheat (Triticum aestivum L.) seed germination

Cited by 9 publications

References 20 publications

Association mapping reveals novel genes and genomic regions controlling grain size architecture in mini core accessions of Indian National Genebank wheat germplasm collection

Association mapping reveals novel genes and genomic regions controlling grain size architecture in mini core accessions of Indian National Genebank wheat germplasm collection

Genome-wide identification, characterization, evolution, and expression pattern analyses of the typical thioredoxin gene family in wheat (Triticum aestivum L.)

Defense Responses of Wheat Seedling under Biotic Stress Mimicked by the Linear β-(1,3)-Glucan

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