The Role of the Pinhole in Confocal Imaging Systems

Wilson, Tony

doi:10.1007/978-1-4615-7133-9_11

Cited by 204 publications

(239 citation statements)

References 12 publications

Supporting

Mentioning

231

Contrasting

Unclassified

Order By: Relevance

“…In the present work we investigate the restructuring of transient gels by means of uorescence Confocal Scanning Laser Microscopy (CSLM) [20]. This powerful technique allows a direct visualization of the structure, both in situ and time-resolved [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Transient gels in colloid–polymer mixtures studied with fluorescence confocal scanning laser microscopy

Verhaegh

Asnaghi

Lekkerkerker

1999

Physica A: Statistical Mechanics and its Applications

View full text Add to dashboard Cite

We study the structure and the time evolution of transient gels formed in colloid-polymer mixtures, by means of uorescence Confocal Scanning Laser Microscopy (CSLM). This technique is used in conjunction with novel colloidal silica particles containing a uorescent core. The confocal micrographs reveal that there exist large di erences in the local structure within a single system. At a given time there are regions where the gel structure consists of alternating patterns of colloid-rich and colloid-poor regions with a characteristic length scale and regions where the gel structure becomes disrupted by the formation of fractures. The number of fractures increases with time. It is speculated that the increase of the number of fractures leads to a weakening of the strength of the gel such that it eventually collapses under gravity.

show abstract

Section: Introductionmentioning

confidence: 99%

Transient gels in colloid–polymer mixtures studied with fluorescence confocal scanning laser microscopy

Verhaegh

Asnaghi

Lekkerkerker

1999

Physica A: Statistical Mechanics and its Applications

View full text Add to dashboard Cite

show abstract

“…During the last few decades, in an attempt to visualize biological structures with a light microscope, several schemes of microscope optics have been devised, for example, phase-contrast optics, dark-field optics, polarization optics, differential interference contrast optics and fluorescence optics (reviewed in Inoue 1986), and more recently confocal optics (reviewed in Brakenhoff et al 1989;Wilson 1990). Among these light microscope optics, fluorescence microscopy, with its molecular selectivity in imaging, uniquely provides a method for observing specific molecular components.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of chromosomes and microtubules visualized by multiple‐wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression

Haraguchi¹,

Kaneda

Hiraoka³

1997

Genes to Cells

View full text Add to dashboard Cite

Background: Mitotic events are accomplished by a coordinated interaction of molecular components within a cell. The continuous observation of specific molecular components in individual living cells provides a unique opportunity to examine the temporal and spatial coordination of these components. In order to be able to observe dynamic events in living mammalian cells, we have developed a computerized fluorescence microscope system.

show abstract

“…The three-dimensional structure of living tissue and solid materials can now be observed directly by imaging techniques such as confocal microscopy (Wilson, 1990). Existing statistical methods for analysing 'spatial' point patterns (Ripley (1981(Ripley ( , 1988, Diggle (1983) and Cressie (1991), chapter 8) are mostly two dimensional; although the general theory works in d dimensions, there are problems for d > 2 with the complexity of edge correction, increased bias and variance due to edge effects, and extra computational load.…”

Section: Introductionmentioning

confidence: 99%

Analysis of a Three-Dimensional Point Pattern with Replication

Baddeley¹,

Moyeed²,

Howard³

et al. 1993

Applied Statistics

112

158

View full text Add to dashboard Cite

SUMMARYTechniques for analysing three-dimensional spatial point patterns are demonstrated on data from a confocal microscope recording the locations of cells in three dimensions. New computational techniques are proposed for edge corrections and empty space measurement. A novel feature of the data is replication and nesting in a sampling design: multiple spatial patterns were observed from each of several animals. For this we develop a ratio regression approach.

show abstract

The Role of the Pinhole in Confocal Imaging Systems

Cited by 204 publications

References 12 publications

Transient gels in colloid–polymer mixtures studied with fluorescence confocal scanning laser microscopy

Transient gels in colloid–polymer mixtures studied with fluorescence confocal scanning laser microscopy

Dynamics of chromosomes and microtubules visualized by multiple‐wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression

Analysis of a Three-Dimensional Point Pattern with Replication

Contact Info

Product

Resources

About