The Role of the Outer Surface of the Plasma Membrane in Aluminum Tolerance

Wagatsuma, Tadao; Ishikawa, Shinya; Ofei-Manu, Paul

doi:10.1007/978-4-431-66902-9_7

Cited by 2 publications

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“…These investigators also suggested that the excretion site of mucilage, localized at the root tip (the apical 1 mm), was induced by another factor because the distal part (2–3 mm from the root tip) is the most Al‐sensitive site of the maize root apex (Sivaguru & Horst, 1998). Wagatsuma et al. (2001) reported no detectable differences in the amount of root mucilage between Al‐tolerant and Al‐sensitive cultivars of maize, wheat, rice ( Oryza sativa L.), pea ( Pisum sativum L.), and sorghum ( Sorghum bicolor (L.) Moench).…”

Section: Introductionmentioning

confidence: 99%

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

et al. 2008

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Summary• Plant roots exude viscous polysaccharides, called mucilage. One of the suggested roles of mucilage is immobilization of toxic metal cations, including aluminum (Al), in the rhizosphere.• Mucilage exuded from roots of Melastoma malabathricum (Al accumulator) was characterized in comparison with that of Zea mays (maize; Al nonaccumulator).• Removal of mucilage significantly reduced Al accumulation in M. malabathricum. The cation adsorption affinity of M. malabathricum mucilage was higher for Al and lanthanum (La) than for barium (Ba), whereas that of maize mucilage was in the order Ba > La > Al. A 27 Al nuclear magnetic resonance (NMR) spectrum of the Al-adsorbed mucilage and bioassay with alfalfa seedlings indicated that the concentrated Al in the mucilage of M. malabathricum, unlike that of maize, bound very weakly to cation exchange sites of mucilage.• The higher charge density in M. malabathricum mucilage, derived from unmethylated uronic acid, is inferred to be related to preferential adsorption of trivalent cation. Not only a higher degree of methylation in the uronic acid (glucuronic acid) but also H + release from roots to the mucilage appears to be responsible for the loose binding of Al in M. malabathricum mucilage. These characteristics of mucilage may help Al hyperaccumulation in M. malabathricum.

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Section: Introductionmentioning

confidence: 99%

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

et al. 2008

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A New and Simple Technique for the Isolation of Plasma Membrane Lipids from Root-Tips

Wagatsuma

Uemura

Mitsuhashi

et al. 2005

Soil Science and Plant Nutrition

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It has been suggested that plasma membrane (PM) lipids play a major role in aluminum (Al) tolerance; however, no direct investigations have been carried out using PM lipids from root‐tips. Here we report a new technique for PM isolation as an alternative to the laborious two‐polymer phase partitioning method that is commonly applied, as follows: 1) separation of protoplasts from 1‐cm root‐tip portions by enzymatic digestion, 2) attachment of the purified protoplasts to glass plates coated with polylysine, 3) preparation of PM ghosts by successive burst of the attached protoplasts using three separate buffer solutions (25 mM PIPES, 5 mM EDTA, and 2 mM MgCl2, at pH 7.0) with slow stirring for 60 s. The PMs were confirmed to be devoid of organelle membranes by fluorescence microscopy, thin layer chromatography (TLC) and western blot analysis. The PM lipids obtained were found to be useful for studies on their differential permeability and lipid composition between lines of triticale or cultivars of maize under Al stress.

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The Role of the Outer Surface of the Plasma Membrane in Aluminum Tolerance

Cited by 2 publications

References 50 publications

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

A New and Simple Technique for the Isolation of Plasma Membrane Lipids from Root-Tips

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