The Role of the Neuropilins in Developmental Angiogenesis

Brash, James T.; Lampropoulou, Anastasia; Ruhrberg, Christiana

doi:10.1007/978-3-319-48824-0_6

Cited by 2 publications

(2 citation statements)

References 66 publications

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“…The categorization and choice of various factors were based on grouping different molecules/factors into groups involved in similar EPC and EC functions. The first group is involved in developmental angiogenesis, tumor angiogenesis, and vascular development; this group comprises neuropilins (NRP1 and NRP2), semaphorins (3A, 3B, 3D, 3E, 3F, 4A, 4D, 5A, and 6A), and VEGFR1, 2, and 3 [32][33][34][35]. The second group is implicated in ECs/EPCs-immune cell interaction, proliferation, migration, survival, apoptosis, angiogenesis, immunogenicity, and immune-modulation.…”

Section: Introductionmentioning

confidence: 99%

Single-cell transcriptomic profiling and characterization of endothelial progenitor cells: new approach for finding novel markers

et al. 2021

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Background Endothelial progenitor cells (EPCs) are promising candidates for the cellular therapy of peripheral arterial and cardiovascular diseases. However, hitherto there is no specific marker(s) defining precisely EPCs. Herein, we are proposing a new in silico approach for finding novel EPC markers. Methods We assembled five groups of chosen EPC-related genes/factors using PubMed literature and Gene Ontology databases. This shortened database of EPC factors was fed into publically published transcriptome matrix to compare their expression between endothelial colony-forming cells (ECFCs), HUVECs, and two adult endothelial cell types (ECs) from the skin and adipose tissue. Further, the database was used for functional enrichment on Mouse Phenotype database and protein-protein interaction network analyses. Moreover, we built a digital matrix of healthy donors’ PBMCs (33 thousand single-cell transcriptomes) and analyzed the expression of these EPC factors. Results Transcriptome analyses showed that BMP2, 4, and ephrinB2 were exclusively highly expressed in EPCs; the expression of neuropilin-1 and VEGF-C were significantly higher in EPCs and HUVECs compared with other ECs; Notch 1 was highly expressed in EPCs and skin-ECs; MIR21 was highly expressed in skin-ECs; PECAM-1 was significantly higher in EPCs and adipose ECs. Moreover, functional enrichment of EPC-related genes on Mouse Phenotype and STRING protein database has revealed significant relations between chosen EPC factors and endothelial and vascular functions, development, and morphogenesis, where ephrinB2, BMP2, and BMP4 were highly expressed in EPCs and were connected to abnormal vascular functions. Single-cell RNA-sequencing analyses have revealed that among the EPC-regulated markers in transcriptome analyses, (i) ICAM1 and Endoglin were weekly expressed in the monocyte compartment of the peripheral blood; (ii) CD163 and CD36 were highly expressed in the CD14+ monocyte compartment whereas CSF1R was highly expressed in the CD16+ monocyte compartment, (iii) L-selectin and IL6R were globally expressed in the lymphoid/myeloid compartments, and (iv) interestingly, PLAUR/UPAR and NOTCH2 were highly expressed in both CD14+ and CD16+ monocytic compartments. Conclusions The current study has identified novel EPC markers that could be used for better characterization of EPC subpopulation in adult peripheral blood and subsequent usage of EPCs for various cell therapy and regenerative medicine applications.

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Section: Introductionmentioning

confidence: 99%

Single-cell transcriptomic profiling and characterization of endothelial progenitor cells: new approach for finding novel markers

et al. 2021

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“…The categorization and choice of various factors were based on grouping different molecules/factors into groups involved in similar EPCs and ECs functions. The rst group is involved in developmental angiogenesis, tumor angiogenesis and vascular development, this group comprises neuropilins (NRP1 & NRP2), semaphorins (3A, 3B, 3D, 3E, 3F, 4A, 4D, 5A & 6A), and VEGFR1, 2 &3 [32][33][34][35]. The second group is implicated in ECs/EPCs-immune cells interaction, proliferation, migration, survival, apoptosis, angiogenesis, immunogenicity and immune-modulation.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptomic Profiling and Characterization of Endothelial Progenitor Cells: New Approach for Finding Novel Markers

Abdelgawad

Desterke

Uzan

et al. 2020

Preprint

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Background: Endothelial progenitor cells (EPCs) are promising candidates for the cellular therapy of peripheral arterial & cardiovascular diseases. However, hitherto there is no specific marker(s) defining precisely EPCs. Herein, we are proposing a new in-silico approach for finding novel EPCs markers. Methods: we assembled five groups of chosen EPCs-related genes/factors using Pubmed literature & gene ontology databases. This shortened database of EPCs factors was fed into publically-published transcriptome matrix to compare their expression between endothelial colony-forming cells (ECFCs), HUVECs and two adult endothelial cell types (ECs) from skin and adipose tissue. Further, the database was used for functional enrichment on mouse phenotype database and protein-protein interaction network analyses. Moreover, we built a digital matrix of healthy donors' PBMCs (33 thousand single cell transcriptomes) and analyzed the expression of these EPCs factorsResults: Transcriptome analyses showed that BMP2,4 & ephrinB2 were exclusively highly expressed in EPCs; the expression of neuropilin-1 & VEGF-C were significantly higher in EPCs & HUVECs compared with other ECs; Notch 1 was highly expressed in EPCs & skin-ECs; MIR21 was highly expressed in skin-ECs; PECAM-1 were significantly higher in EPCs & adipose ECs. Moreover, functional enrichment of EPCs-related genes on mouse phenotype and STRING protein database has revealed significant relations between chosen EPCs factors and endothelial & vascular functions, development and morphogenesis, where ephrinB2, BMP2 and BMP4 were highly expressed in EPCs and were connected to abnormal vascular functions. Single cell RNA-sequencing analyses has revealed that among the EPCs regulated markers in transcriptome analyses: i-ICAM1 & Endoglin were weekly expressed in the monocyte compartment of peripheral blood; ii-CD163 & CD36 were highly expressed in CD14+ monocyte compartment whereas CSF1R was highly expressed in CD16+ monocyte compartment; iii-L-selectin & IL6R were globally expressed in the lymphoid/myeloid compartments; iv-interestingly, PLAUR/UPAR & NOTCH2 were highly expressed in both CD14+ & CD16+ monocytic compartments. Conclusions: The current study has identified novel EPCs markers that could be used for better characterization of EPCs sub-population in adult peripheral blood and subsequent usage of EPCs for various cell therapy and regenerative medicine applications.

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The Role of the Neuropilins in Developmental Angiogenesis

Cited by 2 publications

References 66 publications

Single-cell transcriptomic profiling and characterization of endothelial progenitor cells: new approach for finding novel markers

Single-cell transcriptomic profiling and characterization of endothelial progenitor cells: new approach for finding novel markers

Single-Cell Transcriptomic Profiling and Characterization of Endothelial Progenitor Cells: New Approach for Finding Novel Markers

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