The Role of the Microbiota in Regeneration-Associated Processes

Díaz-Díaz, Lymarie M.; Rodríguez-Villafañe, Andrea; García‐Arrarás, José E.

doi:10.3389/fcell.2021.768783

Cited by 5 publications

(2 citation statements)

References 201 publications

(253 reference statements)

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“…Although we have not conducted experiments in this regard, it is reasonable to expect that the microbiota exerts a decisive influence on regenerative processes. In addition to the multitude of models in which the microbiota has been shown to be involved in regeneration processes 57 , this question has already been addressed in two studies with planarians. In Schmidtea mediterranea bacterial infection and dysbiosis induced by tissue lesioning impaired the regeneration process 58 .…”

Section: Discussionmentioning

confidence: 99%

The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

Sieber

et al. 2023

Commun Biol

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The close association between animals and their associated microbiota is usually beneficial for both partners. Here, we used a simple marine model invertebrate, the flatworm Macrostomum lignano, to characterize the host-microbiota interaction in detail. This analysis revealed that the different developmental stages each harbor a specific microbiota. Studies with gnotobiotic animals clarified the physiological significance of the microbiota. While no fitness benefits were mediated by the microbiota when food was freely available, animals with microbiota showed significantly increased fitness with a reduced food supply. The microbiota of M. lignano shows circadian rhythmicity, affecting both the total bacterial load and the behavior of specific taxa. Moreover, the presence of the worm influences the composition of the bacterial consortia in the environment. In summary, the Macrostomum-microbiota system described here can serve as a general model for host-microbe interactions in marine invertebrates.

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Section: Discussionmentioning

confidence: 99%

The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

Sieber

et al. 2023

Commun Biol

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“…These studies revealed that some bacterial species were shown to be causally linked with the regenerative ability of the model animals. For instance, Pseudomonas and Aquitalea species on Planaria, Erwinia carotovara on fruit fly, Aeromonas veronii on Zebrafish, Akkermansia muciniphila and some probiotic Lactobacillus species in intestinal cell proliferation 16 . The microbiome, therefore, influence tissue regeneration by modulating immune responses and inflammation, and potentially contributing to the activation of regenerative pathways.…”

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confidence: 99%

Temporal Microbiome Changes in Axolotl Limb Regeneration: Stage-Specific Restructuring of Bacterial and Fungal Communities with aFlavobacteriumBloom During Blastema Proliferation

Altın,

Delice,

Yıldırım

et al. 2024

Preprint

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The intricate relationship between regeneration and microbiota has recently gained attention, spanning diverse model organisms. Axolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite its significance, a noticeable gap exists in understanding the interplay between axolotl regeneration and its microbiome. Here, we analyze in depth bacterial 16S rRNA amplicon dataset that we reported before as data resource and profile fungal community by sequencing ITS amplicons at the critical stages of limb regeneration (0-1-4-7-30-60 days post amputation, dpa). Results reveal a decline in richness and evenness in the course of limb regeneration, with bacterial community richness recovering beyond 30 dpa unlike fungi community. Beta diversity analysis reveals precise restructuring of the bacterial community along the three phases of limb regeneration, contrasting with less congruent changes in the fungal community. Temporal dynamics of the bacterial community highlight prevalent anaerobic bacteria in initiation phase and Flavobacterium bloom in the early phase correlating with limb blastema proliferation. Predicted functional analysis mirrors these shifts, emphasizing a transition from amino acid metabolism to lipid metabolism control. Fungal communities shift from Blastomycota to Ascomycota dominance in the late regeneration stage. Our findings provide ecologically relevant insights into stage specific role of microbiome contributions to axolotl limb regeneration.

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Occlusive membranes for guided regeneration of inflamed tissue defects

Choi,

Mangal,

Park

et al. 2023

Nat Commun

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Guided bone regeneration aided by the application of occlusive membranes is a promising therapy for diverse inflammatory periodontal diseases. Symbiosis, homeostasis between the host microbiome and cells, occurs in the oral environment under normal, but not pathologic, conditions. Here, we develop a symbiotically integrating occlusive membrane by mimicking the tooth enamel growth or multiple nucleation biomineralization processes. We perform human saliva and in vivo canine experiments to confirm that the symbiotically integrating occlusive membrane induces a symbiotic healing environment. Moreover, we show that the membrane exhibits tractability and enzymatic stability, maintaining the healing space during the entire guided bone regeneration therapy period. We apply the symbiotically integrating occlusive membrane to treat inflammatory-challenged cases in vivo, namely, the open and closed healing of canine premolars with severe periodontitis. We find that the membrane promotes symbiosis, prevents negative inflammatory responses, and improves cellular integration. Finally, we show that guided bone regeneration therapy with the symbiotically integrating occlusive membrane achieves fast healing of gingival soft tissue and alveolar bone.

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The Role of the Microbiota in Regeneration-Associated Processes

Cited by 5 publications

References 201 publications

The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

Temporal Microbiome Changes in Axolotl Limb Regeneration: Stage-Specific Restructuring of Bacterial and Fungal Communities with aFlavobacteriumBloom During Blastema Proliferation

Occlusive membranes for guided regeneration of inflamed tissue defects

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