The role of the MAP kinase−kinase protein StMKK1 in potato immunity to different pathogens

Chen, Xiaokang; Wang, Wenbin; Cai, Pingping; Wang, Ziwei; Li, Tingting; Du, Yuguang

doi:10.1038/s41438-021-00556-5

Cited by 26 publications

(19 citation statements)

References 44 publications

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“…In addition, the roles of cell wall proteins in defense against R. solanacearum in tomato have been discussed extensively ( Wydra and Beri, 2006 ; Diogo and Wydra, 2007 ; Dahal et al, 2010 ; Schacht et al, 2011 ). Moreover, several recent studies have identified genes related to plant defense against R. solanacearum , including StMKK1 in potato ( Chen et al, 2021 ), SlNAP1 and a bacterial effector protein RipAK in tomato ( Wang et al, 2020 ; Wang Y. et al, 2021 ), and CaNAC2c in pepper ( Cai et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

et al. 2021

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Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we characterized the physiological differences and compared the defense-related transcriptomes of two tobacco varieties, 4411-3 (highly resistant, HR) and K326 (moderately resistant, MR), after R. solanacearum infection at 0, 10, and 17 days after inoculation (dpi). A total of 3967 differentially expressed genes (DEGs) were identified between the HR and MR genotypes under mock condition at three time points, including1395 up-regulated genes in the HR genotype and 2640 up-regulated genes in the MR genotype. Also, 6,233 and 21,541 DEGs were induced in the HR and MR genotypes after R. solanacearum infection, respectively. Furthermore, GO and KEGG analyses revealed that DEGs in the HR genotype were related to the cell wall, starch and sucrose metabolism, glutathione metabolism, ABC transporters, endocytosis, glycerolipid metabolism, and glycerophospholipid metabolism. The defense-related genes generally showed genotype-specific regulation and expression differences after R. solanacearum infection. In addition, genes related to auxin and ABA were dramatically up-regulated in the HR genotype. The contents of auxin and ABA in the MR genotype were significantly higher than those in the HR genotype after R. solanacearum infection, providing insight into the defense mechanisms of tobacco. Altogether, these results clarify the physiological and transcriptional regulation of R. solanacearum resistance infection in tobacco, and improve our understanding of the molecular mechanism underlying the plant-pathogen interaction.

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Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

et al. 2021

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“…MAPKs are targets of virulent and avirulent effectors (reviewed by Lang and Colcombet, 2020 ). Chen et al. (2021) demonstrated that silencing the StMKK1 encoding a MAP kinase kinase in potato results in almost symptomless Rs infection.…”

Section: Discussionmentioning

confidence: 99%

“…MAPKs are targets of virulent and avirulent effectors (reviewed by Lang and Colcombet, 2020). Chen et al (2021) demonstrated that silencing the StMKK1 encoding a MAP kinase kinase in potato results in almost symptomless Rs infection. We found MAPK9 MEKK1 in CG, MEKK7 in CR, and MEKK EDR1 in both cultivars to be downregulated suggesting that reducing the activity of MAPKs may be a good strategy to alleviate losses caused by Rs.…”

Section: Miscellaneous Pathways In Rs Resistancementioning

confidence: 99%

“…Cao et al (2020) studied the early response of S. tuberosum to Rs in leaves at 2 dpi and found differentially expressed genes, including LRRs and HSPs, between the control and the Rsinfected sample, as well as genes involved in the biosynthesis of amino acids, plant hormone signal transduction, and starch and sucrose metabolism. Recently, Chen et al (2021) silenced StMKK1, a mitogen-activated protein (MAP) kinase kinase in potato, which resulted in enhanced PTI and SA-related immune responses. The StMKK1-silenced lines developed almost no symptoms when tested at 5 dpi.…”

Section: Introductionmentioning

confidence: 99%

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Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

et al. 2023

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Ralstonia solanacearum (Rs), the causal agent of bacterial wilt disease in an unusually wide range of host plants, including potato (Solanum tuberosum), is one of the most destructive phytopathogens that seriously reduces crop yields worldwide. Identification of defence mechanisms underlying bacterial wilt resistance is a prerequisite for biotechnological approaches to resistance breeding. Resistance to Rs has been reported only in a few potato landraces and cultivars. Our in vitro inoculation bioassays confirmed that the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to Rs infection. Comparative transcriptome analyses of CG and CR roots, as well as of the roots of an Rs-susceptible cultivar, ‘Désirée’ (DES), were carried out two days after Rs infection, in parallel with their respective noninfected controls. In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected. The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in CR, as confirmed by high levels of lignification over the whole stele in CR roots six days after Rs infection. At the same time, Rs infection greatly increased the concentrations of chlorogenic acid and quercetin derivatives in CG roots as it was detected using ultra-performance liquid chromatography − tandem mass spectrometry. Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots. These results indicate different Rs defence mechanisms in the two resistant potato cultivars and a different response to Rs infection in the susceptible cultivar.

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“…Our previous studies showed that P. infestans RXLR effector targets and stabilizes StMKK1 to facilitate pathogen colonization [ 33 ]. Further investigation reveals that StMKK1 negatively regulates potato immunity to biotrophic and hemi-biotrophic pathogens by repressing PTI and SA-related signaling pathways [ 34 ]. The StMKK1 downstream signalling target StMPK7 was found to positively regulate plant defence to Phytophthora pathogens via the SA signalling pathway [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

Zhang

et al. 2022

Horticulture Research

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Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in regulating plant immunity. MAPKs usually transduce signals and regulate plant immunity by phosphorylating the downstream defence-related components. Our previous study indicates that StMPK7 positively regulates plant defence to Phytophthora pathogens via SA signalling pathway. However, the downstream component of StMPK7 remains unknown. In this study, we employed GFP-StMPK7 transgenic potato and performed immunoprecipitation-mass spectrometry (IP-MS) to identify the downstream component of StMPK7. We found that an RNA binding protein StUBA2a/b interacted with StMPK7, as revealed by luciferase complementation imaging (LCI) and coimmunoprecipitation (co-IP) assays. Transient expression of StUBA2a/b in N. benthamiana enhanced plant resistance to Phytophthora pathogens, while silencing of UBA2a/b decreased the resistance, suggesting a positive regulator role of UBA2a/b in plant immunity. Similar to StMPK7, StUBA2a/b was also involved in SA signalling pathway and induced SGT1-dependent cell death as constitutively activated (CA)-StMPK7 did. Immune blotting indicated that StMPK7 phosphorylates StUBA2a/b at thr248 and thr408 (T248/408) sites and stabilizes StUBA2a/b. Silencing of MPK7 in N. benthamiana suppressed StUBA2a/b-induced cell death, while co-expression with StMPK7 enhanced the cell death. Besides, StUBA2a/bT248/408A mutant showed decreased ability to trigger cell death and elevate the expression of PR genes, indicating the phosphorylation by StMPK7 enhances the functions of StUBA2a/b. Moreover, CA-StMPK7-induced cell death was largely suppressed by silencing of NbUBA2a/b, genetically implying UBA2a/b acts as the downstream component of StMPK7. Collectively, our results reveal that StMPK7 phosphorylates and stabilizes its downstream substrate StUBA2a/b to enhance plant immunity via the SA signalling pathway.

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The role of the MAP kinase−kinase protein StMKK1 in potato immunity to different pathogens

Cited by 26 publications

References 44 publications

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

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