The role of the interplay between polymer architecture and bacterial surface properties on the microbial adhesion to polyoxazoline-based ultrathin films

Pidhatika, Bidhari; Möller, Jens; Benetti, Edmondo M.; Konradi, Rupert; Rakhmatullina, Ekaterina; Mühlebach, Andreas; Zimmermann, Ralf; Werner, Carsten; Vogel, Viola; Textor, Marcus

doi:10.1016/j.biomaterials.2010.08.033

Cited by 115 publications

(126 citation statements)

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“…Textor and co-workers prepared poly(L-lysine)-graft-pMeOx copolymers by attaching carboxyl-terminated pMeOx copolymers to poly(L-lysine) polymers using EDC/sulfo-NHS-assisted coupling [113]. This graft copolymer was used to form surface platforms on Nb 2 O 5 [114] to investigate the adhesion mechanism of Escherichia coli and in particular the influence of fimbriae expression.…”

Section: Grafting Onto Surfacesmentioning

confidence: 99%

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

2013

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Abstract:The polymer class of poly(2-oxazoline)s currently is under intensive investigation due to the versatile properties that can be tailor-made by the variation and manipulation of the functional groups they bear. In particular their utilization in the biomedic(in)al field is the subject of numerous studies. Given the mechanism of the cationic ring-opening polymerization, a plethora of synthetic strategies exists for the preparation of poly(2-oxazoline)s with dedicated functionality patterns, comprising among others the functionalization by telechelic end-groups, the incorporation of substituted monomers into (co)poly(2-oxazoline)s, and polymeranalogous reactions. This review summarizes the current state-of-the-art of poly(2-oxazoline) preparation and showcases prominent examples of poly(2-oxazoline)-based materials, which are retraced to the desktop-planned synthetic strategy and the variability of their properties for dedicated applications.

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Section: Grafting Onto Surfacesmentioning

confidence: 99%

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

2013

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“…Homogeneous DMOAC coating with a dry adlayer thickness of 2.2 nm was confirmed by variable-angle spectroscopic ellipsometry. To allow for a direct contact of the bacterial membrane with the surface-immobilized membrane-active DMOAC molecules, non-fimbriated eGFP expressing E. coli (AAEC191A pHis-GFP) were used [4]. As detected by 3 μM PI staining, all adherent bacteria on the DMOAC surface were killed within 30 minutes of surface incubation and no measurable bacterial growth occurred (Figure 3b, Additional file 1: Figure S2, Additional file 2: Movie S1).…”

Section: Resultsmentioning

confidence: 99%

“…E. coli K-12 derivatives have been widely used as model strains in surface adhesion and biofilm studies [4,31,[37][38][39]. We expressed eGFP from the pHis plasmid under the control of the IPTG inducible tac promoter to replace the growth inhibiting DNA stain SYTO ® 9 as live bacterial marker.…”

Section: Discussionmentioning

confidence: 99%

“…Surface biofouling commonly starts with the adhesion of individual bacteria that subsequently grow into mature biofilms. To prevent bacterial adhesion and growth already during the pre-biofilm phase, two main surface engineering strategies have been developed so far: non-fouling "stealth" surface coatings that inhibit adhesion of proteins and bacteria [2][3][4] and bioactive materials, which upon bacterial contact or release of the active molecules interfere with bacterial viability [5][6][7][8][9][10]. To compare the antimicrobial properties of surface coatings and to study the kinetics of bacterial surface colonization, assays are needed that allow for in situ monitoring of bacterial adhesion and viability.…”

Section: Introductionmentioning

confidence: 99%

“…To kill bacteria, the quaternary ammonium chloride complexes of surface-bound DMOAC have to directly interact with the bacterial membrane [23]. We previously showed that bacterial fimbriae strongly influence the unspecific adhesion of E. coli to engineered surfaces [4]. Type 1 fimbriae (7 nm diameter, several 100 μm length) protrude from the bacterial membrane thereby preventing the bulk bacterial body from direct interaction with the underlying material surface.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence-based in situ assay to probe the viability and growth kinetics of surface-adhering and suspended recombinant bacteria

et al. 2013

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Bacterial adhesion and biofilm growth can cause severe biomaterial-related infections and failure of medical implants. To assess the antifouling properties of engineered coatings, advanced approaches are needed for in situ monitoring of bacterial viability and growth kinetics as the bacteria colonize a surface. Here, we present an optimized protocol for optical real-time quantification of bacterial viability. To stain living bacteria, we replaced the commonly used fluorescent dye SYTO ® 9 with endogenously expressed eGFP, as SYTO ® 9 inhibited bacterial growth. With the addition of nontoxic concentrations of propidium iodide (PI) to the culture medium, the fraction of live and dead bacteria could be continuously monitored by fluorescence microscopy as demonstrated here using GFP expressing Escherichia coli as model organism. The viability of bacteria was thereby monitored on untreated and bioactive dimethyloctadecyl [3-(trimethoxysilyl)propyl]ammonium chloride (DMOAC)-coated glass substrates over several hours. Pre-adsorption of the antimicrobial surfaces with serum proteins, which mimics typical protein adsorption to biomaterial surfaces upon contact with host body fluids, completely blocked the antimicrobial activity of the DMOAC surfaces as we observed the recovery of bacterial growth. Hence, this optimized eGFP/PI viability assay provides a protocol for unperturbed in situ monitoring of bacterial viability and colonization on engineered biomaterial surfaces with single-bacteria sensitivity under physiologically relevant conditions.

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Surface Functionalization of Biomaterials by Poly(2‐oxazoline)s

Morgese

Benetti

2017

Polymers for Biomedicine

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The role of the interplay between polymer architecture and bacterial surface properties on the microbial adhesion to polyoxazoline-based ultrathin films

Cited by 115 publications

References 58 publications

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

Fluorescence-based in situ assay to probe the viability and growth kinetics of surface-adhering and suspended recombinant bacteria

Surface Functionalization of Biomaterials by Poly(2‐oxazoline)s

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