The role of the immune system in the pathogenesis of rosacea

Горбакова, Е. В.; Masyukova, S. A.; Ilyina, I.V.; Arzumanyan, V. G.

doi:10.17116/klinderma202120041112

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…Infections caused by H. pylori , such as intestinal cancer, have been shown to produce high levels of alpha 1, 2, and 3 defensins, as well as beta-defensins (Inthasin et al, 2019 ; Kharayat et al, 2019 ). The use of these defensins in in-silico cloning may result in higher fold expression of the defensins (SchöNiger et al, 2018 ; Gorbakova et al, 2021 ). Given the harmful function that these proteins play in H. pylori infections, they are more suitable candidates for use in the development of vaccines that will offer long-term protection against H. pylori infections (Boyd et al, 2021 ; Li et al, 2016 ; Goyffon et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Immunoinformatic prediction of potential immunodominant epitopes from cagW in order to investigate protection against Helicobacter pylori infection based on experimental consequences

Chehelgerdi

Heidarnia

Dehkordi

et al. 2023

Funct Integr Genomics

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Helicobacter pylori is a leading cause of stomach cancer and peptic ulcers. Thus, identifying epitopes in H. pylori antigens is important for disease etiology, immunological surveillance, enhancing early detection tests, and developing optimal epitope-based vaccines. We used immunoinformatic and computational methods to create a potential CagW epitope candidate for H. pylori protection. The cagW gene of H. pylori was amplified and cloned into pcDNA3.1 (+) for injection into the muscles of healthy BALB/c mice to assess the impact of the DNA vaccine on interleukin levels. The results will be compared to a control group of mice that received PBS or cagW-pcDNA3.1 (+) vaccinations. An analysis of CagW protein antigens revealed 8 CTL and 7 HTL epitopes linked with AYY and GPGPG, which were enhanced by adding B-defensins to the N-terminus. The vaccine’s immunogenicity, allergenicity, and physiochemistry were validated, and its strong activation of TLRs (1, 2, 3, 4, and 10) suggests it is antigenic. An in-silico cloning and immune response model confirmed the vaccine’s expression efficiency and predicted its impact on the immune system. An immunofluorescence experiment showed stable and bioactive cagW gene expression in HDF cells after cloning the whole genome into pcDNA3.1 (+). In vivo vaccination showed that pcDNA3.1 (+)-cagW-immunized mice had stronger immune responses and a longer plasmid DNA release window than control-plasmid-immunized mice. After that, bioinformatics methods predicted, developed, and validated the three-dimensional structure. Many online services docked it with Toll-like receptors. The vaccine was refined using allergenicity, antigenicity, solubility, physicochemical properties, and molecular docking scores. Virtual-reality immune system simulations showed an impressive reaction. Codon optimization and in-silico cloning produced E. coli-expressed vaccines. This study suggests a CagW epitopes-protected H. pylori infection. These studies show that the proposed immunization may elicit particular immune responses against H. pylori , but laboratory confirmation is needed to verify its safety and immunogenicity.

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