The Role of the Ganglion Cells in the Small Intestine Taken in the Intestinal Intrinsic Reflex

Hukuhara, Takehiko; Sumi, Tadaaki; Kotani, Satoru

doi:10.2170/jjphysiol.11.281

Cited by 40 publications

(8 citation statements)

References 7 publications

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“…Usually denervation can be achieved surgically [11], by prolonged anoxia [22], and by infection as in Chagas' disease [23]. These methods are generally limited to acute in vitro preparations, and technically difficult in small experimental animals.…”

Section: Discussionmentioning

confidence: 99%

Morphological Alterations in Small Intestine of Rats with Myenteric Plexus Denervation

Deniz

Kilinc²,

Hatipoğlu³

2004

Eur Surg Res

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We aimed to investigate the effect of myenteric denervation by benzalkonium chloride (BAC) on small intestine morphology in the rat, and whether segmental myenteric denervation alters morphology elsewhere in the small intestine. Forty male Sprague-Dawley rats were equally divided into 4 groups: control (0.9% NaCl); denervation (0.062% BAC); chemical inflammation (5% acetic acid), and intraluminal stasis produced by partial obstruction. 28 days after operation tissue samples were taken from the treated segment, 10 cm distal to the treated segment, and 20 cm proximal to the treated segment. Morphological changes and the number of ganglion cells were examined under the light microscope. BAC application reduced the number of myenteric neurons by 85% in the treated segment. Denervation increased villus height and crypt depth in the treated and proximal segments. But changes in muscle thickness were seen throughout the intestine. As a result, although myenteric plexus denervation caused mucosa morphology in the treated and proximal segments, it caused smooth muscle changes throughout the small intestine.

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Section: Discussionmentioning

confidence: 99%

Morphological Alterations in Small Intestine of Rats with Myenteric Plexus Denervation

Deniz

Kilinc²,

Hatipoğlu³

2004

Eur Surg Res

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“…Enteric neurons are also present within the GI tract; enteric neurons innervate the smooth muscle and are essential for peristalsis [14–16]. This suggests that enteric neurons might act in concert with ICCs to mediate the various patterns of GI motility [17–20].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Formation of Enteric Neural Network Structure in a Gut-Like Organ Differentiated from Mouse Embryonic Stem Cells

et al. 2006

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“…Enteric neurons also innervate smooth muscle and are essential for peristalsis in GI motility (1). Thus ICC and/or enteric neurons could coordinate GI motility (8,9,14,21,22). ES cells have a pluripotent ability to differentiate into a wide range of cell types, and thus the types of ES clusters are heterogeneous.…”

mentioning

confidence: 99%

Characterization of in vitro gutlike organ formed from mouse embryonic stem cells

Ishikawa

Nakayama²,

Nakagawa³

et al. 2004

American Journal of Physiology-Cell Physiology

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Using an embryoid body (EB) culture system, we have made a functional organlike cluster: the "gut" from embryonic stem (ES) cells (ES gut). There are many types of ES clusters, because ES cells have a pluripotent ability to develop into a wide range of cell types. Before inducing specific differentiation by exogenously added factors, we characterized comprehensive physiological and morphological properties of ES guts. Each ES gut has a hemispherical (or cystic) structure and exhibits spontaneous contractions [mean frequency: 13.5 +/- 8.8 cycles per min (cpm)]. A dense distribution of interstitial cells of Cajal (ICC) was identified by c-Kit immunoreactivity, and specific subcellular structures of ICC and smooth muscle cells were identified with electron microscopy. ICC frequently formed close contacts with the neighboring smooth muscle cells and occasionally formed gap junctions with other ICC. Widely propagating intracellular Ca(2+) concentration oscillations were generated in the ES gut from the aggregates of c-Kit immunopositive cells. Plateau potentials, possibly pacemaker potentials in ICC, and electrical slow waves were recorded for the first time. These events were nifedipine insensitive, as in the mouse gut. Our present results indicate that the rhythmic pacemaker activity generated in ICC efficiently spreads to smooth muscle cells and drives spontaneous rhythmic contractions of the ES gut. The present characterization of physiological and morphological properties of ES gut paves the way for making appropriate models to investigate the origin of rhythmicity in the gut.

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The Role of the Ganglion Cells in the Small Intestine Taken in the Intestinal Intrinsic Reflex

Cited by 40 publications

References 7 publications

Morphological Alterations in Small Intestine of Rats with Myenteric Plexus Denervation

Morphological Alterations in Small Intestine of Rats with Myenteric Plexus Denervation

In Vitro Formation of Enteric Neural Network Structure in a Gut-Like Organ Differentiated from Mouse Embryonic Stem Cells

Characterization of in vitro gutlike organ formed from mouse embryonic stem cells

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