The role of the enzyme-modified comet assay in in vivo studies

Collins, Andrew; Vettorazzi, Ariane; Azqueta, Amaya

doi:10.1016/j.toxlet.2020.03.016

Cited by 8 publications

(2 citation statements)

References 65 publications

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“…When the ability of cells to eliminate and repair damage is compromised by excessive production of Reactive Oxygen Species ROS, macromolecules (DNA, lipids, and proteins) are oxidized [ 22 ]. However, the classical comet assay is generally not sensitive enough to detect DNA oxidative bases, and the addition of enzymes such as Fpg and OGG1 is required [ 23 ]. Nevertheless, in our study, the Fpg-modified comet assay in the spleen did not reveal any increase in DNA oxidative bases after oral exposure to C17-SAMT.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Genotoxic Potential of the Marine Toxin C17-SAMT Using the In Vivo Comet and Micronucleus Assays

et al. 2022

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The contaminant responsible for the atypical toxicity reported in mussels from Bizerte Lagoon (Northern Tunisia) during the last decade has been characterized as C17-sphinganine analog mycotoxin (C17-SAMT). This neurotoxin showed common mouse toxic symptoms, including flaccid paralysis and severe dyspnea, followed by rapid death. For hazard assessment on human health, in this work we aimed to evaluate the in vivo genotoxic effects of this marine biotoxin using the classical alkaline and modified Fpg comet assays performed to detect DNA breaks and alkali-labile sites as well as oxidized bases. The micronucleus assay was used on bone marrow to detect chromosome and genome damage. C17-SAMT induces a statistically insignificant increase in DNA tail intensity at all doses in the duodenum, and in the spleen contrary to the liver, the percentage of tail DNA increased significantly at the mid dose of 300 µg/kg b.w/d. C17-SAMT did not affect the number of micronuclei in the bone marrow. Microscopic observations of the liver showed an increase in the number of mitosis and hepatocytes’ cytoplasm clarification. At this level of study, we confirm that C17-SAMT induced DNA damage in the liver but there was no evidence of effects causing DNA oxidation or chromosome and genome damage.

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Section: Discussionmentioning

confidence: 99%

Investigation of the Genotoxic Potential of the Marine Toxin C17-SAMT Using the In Vivo Comet and Micronucleus Assays

et al. 2022

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“…The most common modifications include the use of specific enzymes such as endonuclease III for the evaluation of oxidised pyrimidines, and formamidopyrimidine DNA glycosylase (FPG) for the detection and removal of the oxidatively damaged purines (e.g., 8-OHdG). This procedure allows a more direct measurement of DNA damage within the cells and a quantitative comparison with appropriate control [ 10 , 11 ]. A further comet protocol often utilised enables the evaluation of DNA susceptibility to oxidative damage generally induced by using H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects

et al. 2021

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The use of DNA damage as marker of oxidative stress, metabolic dysfunction and age-related diseases is debated. The present study aimed at assessing the level of DNA damage (evaluated as DNA strand-breaks, endogenous and oxidatively-induced DNA damage) in a group of older subjects with intestinal permeability enrolled within the MaPLE (Gut and Blood Microbiomics for Studying the Effect of a Polyphenol-Rich Dietary Pattern on Intestinal Permeability in the Elderly) intervention trial, to evaluate its association with clinical, metabolic and dietary markers. DNA damage in peripheral blood mononuclear cells was assessed by the comet assay in 49 older subjects participating in the study. Clinical and metabolic markers, markers of inflammation, vascular function and intestinal permeability were determined in serum. Food intake was estimated by weighted food diaries. On the whole, a trend towards higher levels of DNA damage was observed in men compared to women (p = 0.071). A positive association between DNA damage and clinical/metabolic markers (e.g., uric acid, lipid profile) and an inverse association with dietary markers (e.g., vitamin C, E, B6, folates) were found and differed based on sex. By considering the importance of DNA stability during aging, the results obtained on sex differences and the potential role of dietary and metabolic factors on DNA damage underline the need for further investigations in a larger group of older adults to confirm the associations found and to promote preventive strategies.

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Ecotoxicity of single and mixture of perfluoroalkyl substances (PFOS and PFOA) in soils to the earthworm Aporrectodea caliginosa

Delor¹,

Louzon²,

Pelosi

et al. 2023

Environmental Pollution

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The role of the enzyme-modified comet assay in in vivo studies

Cited by 8 publications

References 65 publications

Investigation of the Genotoxic Potential of the Marine Toxin C17-SAMT Using the In Vivo Comet and Micronucleus Assays

Investigation of the Genotoxic Potential of the Marine Toxin C17-SAMT Using the In Vivo Comet and Micronucleus Assays

Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects

Ecotoxicity of single and mixture of perfluoroalkyl substances (PFOS and PFOA) in soils to the earthworm Aporrectodea caliginosa

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