The role of the cAMP pathway in mediating the effect of head activator on nerve-cell determination and differentiation in hydra

Fenger, Ursula; Hofmann, Michael; Galliot, Brigitte; Schaller, Hubert

doi:10.1016/0925-4773(94)90084-1

Cited by 34 publications

(21 citation statements)

References 28 publications

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“…(40±43) After binding to receptors found on the surface of pluripotential interstitial cells, HA induces an increase of intracellular cAMP which then activates protein kinase A, resulting in the phosphorylation of the transcription factor CREB. (44,45) Once modified, CREB activates the genes responsible for the terminal differentiation of nerve cells, which in turn are responsible for the budding process in hydra. (46) Finally, the emerging bud will eventually differentiate and produce a new and complete organism.…”

Section: Asexual Reproduction and Regenerationmentioning

confidence: 99%

Regeneration in the metazoans: why does it happen?

Alvarado

2000

BioEssays

287

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Why does regeneration occur? And why, when it manifests itself, does it do so in some but not all metazoan species? Hence, what are the permissive or inhibitory factors operating behind this phenomenon? When it comes to regeneration, many questions, such as these, remain unanswered. In fact, the problem of animal regeneration has withstood the probing of scientific inquiry for over 250 years and still awaits a satisfactory mechanistic explanation. In this essay, I will review the distribution and the modes of regeneration that are found in the different metazoan phyla. Also, I will re‐examine ideas on its evolutionary origins, and discuss its possible relationship to both asexual reproduction and embryogenesis. This endeavor has two objectives. First, to bring forward an interpretation of regeneration which integrates evolutionary and developmental considerations into its discussion. And second, to suggest a comparative experimental approach to this problem that may bring us closer to understanding the molecular basis of this long‐standing biological problem. BioEssays 22:578–590, 2000. © 2000 John Wiley & Sons, Inc.

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Section: Asexual Reproduction and Regenerationmentioning

confidence: 99%

Regeneration in the metazoans: why does it happen?

Alvarado

2000

BioEssays

287

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“…The sections were collected on gelatin-and polylysine-coated slides, fixed for 20 min in 4% paraformaldehyde/phosphate-buffered saline (PBS), washed three times for 5 min in PBS, air-dried, and stored at -70°C. Macerated cells were prepared [4], and in situ hybridizations were performed as described previously [5,6]. Labeled sense and antisense RNA probes were synthesized from the full-length cDNA as well as from the EcoRI-XhoI and the EcoRI-EcoRI subclones using [ 35 S]-UTP and a Stratagene in vitro transcription kit.…”

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confidence: 99%

Isolation of a marker for head-specific cell differentiation in hydra

et al. 1996

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“…Recent data lend some support to the putative role of cAMP during regeneration, suggesting that activation of the cAMP pathway, including that of the CREB transcription factor, plays a key role in the propagation of the HA ~i g n a I (~~7~~) and also probably that of pedin and pedibin (S. Hoffmeister, unpublished data). cAMP treatment of intact hydra mimics the induction of fast nerve-cell differentiation obtained with either HNFA treatment or w~unding(~+j~); in addition, cAMP exposure induces the determination of nerve precursor cells from the stock of interstitial cells (67). No data are yet available concerning the differentiation of epithelial cells.…”

Section: Head Activator and Cyclic Amp Pathwaymentioning

confidence: 99%

Signaling molecules in regenerating hydra

Galliot

1997

BioEssays

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Ever since it was discovered in hydra, regeneration has remained a stimulating question for developmental biologists. Cellular approaches have revealed that,within the first few hours of apical or basal hydra regeneration, differentiation and determination of nerve cells are the primary cellular events detectable. The head and foot activators (HA, FA), neuropeptides that are released upon injury, are signaling molecules involved in these processes. In conditions where it induces cellular differentiation or determination, HA behaves as an agonist of the cyclic AMP (cAMP) pathway involving the modulation of CREB nuclear transcription factor activity. This cascade would be required for proper regeneration,regardless of whether the polarity involved is apical or basal. Modulations of the protein kinase C pathway, which have been shown to affect apical or basal positional values, might signal to bring about this polarity; however, endogenous ligands responsible for this modulation are as yet unknown.

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The role of the cAMP pathway in mediating the effect of head activator on nerve-cell determination and differentiation in hydra

Cited by 34 publications

References 28 publications

Regeneration in the metazoans: why does it happen?

Regeneration in the metazoans: why does it happen?

Isolation of a marker for head-specific cell differentiation in hydra

Signaling molecules in regenerating hydra

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