The role of the CaCl2–guluronic acid interaction on alginate encapsulated βTC3 cells

Simpson, Nicholas E.; Stabler, Cherie L.; Simpson, Chiab P.; Sambanis, Athanassios; Constantinidis, Ioannis

doi:10.1016/j.biomaterials.2003.09.046

Cited by 144 publications

(109 citation statements)

References 17 publications

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“…The most useful and unique property of alginates was their ability to react with polyvalent metal cations, specifically calcium ions to produce strong gels or insoluble polymers (King, 1983;Simpson et al, 2004). Such calcium-alginate gels are used in the food processing industry for producing restructured foods such as meat products, onion rings, crabsticks and cocktail berries (Moe et al, 1995).…”

Section: Cold-set Binding Systemmentioning

confidence: 99%

Developments in Science, Technology, Quality and Constraints of Restructured Meat Products-A Review

Reddy¹,

Mandal²,

Sen³

et al. 2014

International J. of Meat Science

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To provide the consumer with a highly palatable product at a reasonable cost is the main objective of any food industry. Particular to meat industry, utilization of less valuable carcasses and carcass cuts and carcass components (plates, flanks, shanks, etc.) is of prime interest in periods of economic pressure. One such relevantly new technological approach is restructuring technology, which enables the production of value-added meat products from low value cuts and trimmings of carcasses. Although the economics and processing of restructuring meats appear favourable to producing a product that has the palatability attributes that are between an intact muscle steak and ground meat. This review of restructured meat products discuss the actual science and technology behind the restructuring of muscles, how muscle chunks are binding each together at protein molecules stage, factors influencing the meat pieces binding, type of restructuring methodologies, quality attributes of restructured meat products, problems of oxidation, use of natural antioxidants and recommends research needs for the future.

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Section: Cold-set Binding Systemmentioning

confidence: 99%

Developments in Science, Technology, Quality and Constraints of Restructured Meat Products-A Review

Reddy¹,

Mandal²,

Sen³

et al. 2014

International J. of Meat Science

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show abstract

“…[194][195][196][197] Besides these biological factors, another approach taken to mimic the ECM properties is to directly conjugate ECM molecules to the scaffolds during fabrication or to use decellularized tissues as ECM scaffolds. [198][199][200][201] The ECM can be obtained from decellularized tissues and organs, for example, nerve, liver, and respiratory tract. [202][203][204][205] The advantage of incorporating ECM into the scaffolds is to improve tissue specificity and to facilitate maintenance of cell functions and phenotype.…”

Section: Loh and Choongmentioning

confidence: 99%

Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size

Loh

Choong

2013

Tissue Engineering Part B: Reviews

2,061

1,560

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Tissue engineering applications commonly encompass the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the incorporation of cells or growth factors to regenerate damaged tissues or organs. These scaffolds serve to mimic the actual in vivo microenvironment where cells interact and behave according to the mechanical cues obtained from the surrounding 3D environment. Hence, the material properties of the scaffolds are vital in determining cellular response and fate. These 3D scaffolds are generally highly porous with interconnected pore networks to facilitate nutrient and oxygen diffusion and waste removal. This review focuses on the various fabrication techniques (e.g., conventional and rapid prototyping methods) that have been employed to fabricate 3D scaffolds of different pore sizes and porosity. The different pore size and porosity measurement methods will also be discussed. Scaffolds with graded porosity have also been studied for their ability to better represent the actual in vivo situation where cells are exposed to layers of different tissues with varying properties. In addition, the ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffolds will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix. Overall, the limitations of current scaffold fabrication approaches for tissue engineering applications and some novel and promising alternatives will be highlighted.

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“…At the same time, cell growth characteristics were impeded in alginates with a high guluronic acid content [71]. In contrast, the gel strength of alginates with a high mannuronic acid content was not affected by changes in the concentration of CaCl 2 , because of the low fraction of crosslinking sites provided by the guluronic acid residues.…”

Section: Cell Encapsulation In Alginate Polymersmentioning

confidence: 78%

“…Conversely, high mannuronic acid content alginates provide an environment that supports cell growth [68][69][70]. Simpson et al [71] hypothesized that the strength of the alginate gel network is the principle factor responsible for differences in the growth characteristics of encapsulated cells in different alginates.…”

Section: Cell Encapsulation In Alginate Polymersmentioning

confidence: 99%

“…Gel properties are known to be influenced by the concentration of alginate solution, the duration of gelation, the molarity of the divalent crosslinking solution [158,159] and method of sterilization [160]. Therefore, alginate featuring a high guluronic acid content (G:M ratio 2:1) was selected based on previous studies [160,161], in order to provide mechanically stable cell support [71]. Furthermore, Bohari et al [162] reported that as the concentration of alginate increased to 2 and 5%, major 3T3 fibroblast cell death (30-50%) occurred within the hydrogel matrix at day one while cell viability was maintained in excess of 90% when encapsulated using 0.5 52 or 1% alginate solution.…”

Section: Dimensions and Morphology Of Alginate Fibresmentioning

confidence: 99%

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Production and in vitro evaluation of macroporous alginate hydrogel fibres for nerve tissue engineering

Lin¹

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The prospects for successful peripheral nerve repair using fibre guides are considered to be enhanced by use of a scaffold material which provides a good substrate for attachment and growth of glial cells and regenerating neurons. Alginate polymers exhibit a highly favourable balance of properties and exhibit the unique property of forming hydrogels under mild crosslinking conditions. As a result alginate has been widely applied for cell encapsulation. However the polysaccharide exhibits extremely poor cell adhesion properties, which limits its application for tissue regeneration.

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The role of the CaCl2–guluronic acid interaction on alginate encapsulated βTC3 cells

Cited by 144 publications

References 17 publications

Developments in Science, Technology, Quality and Constraints of Restructured Meat Products-A Review

Developments in Science, Technology, Quality and Constraints of Restructured Meat Products-A Review

Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size

Production and in vitro evaluation of macroporous alginate hydrogel fibres for nerve tissue engineering

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