The role of surface wettability and surface charge of electrosprayed nanoapatites on the behaviour of osteoblasts

Thian, Eng San; Ahmad, Zeeshan; Huang, Jie; Edirisinghe, Mohan; Jayasinghe, Suwan N.; Ireland, Deborah C.; Brooks, Roger A.; Rushton, Neil; Bonfield, W.; Best, Serena M.

doi:10.1016/j.actbio.2009.08.012

Cited by 93 publications

(59 citation statements)

References 30 publications

(26 reference statements)

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“…28,35,43 On the other hand, nano-HAs with higher surface area, smaller crystal size and enhanced bioreactivity, can be prepared avoiding the high temperature sintering process of these bioceramics. 42,[44][45][46] In the present study, the In the three cases, all the diffraction maxima are assigned to a HA phase. The coherent diffraction domains, commonly named crystallite size, were calculated from the Rietveld refinements.…”

Section: Resultsmentioning

confidence: 99%

Effects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatites

et al. 2016

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Section: Resultsmentioning

confidence: 99%

Effects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatites

et al. 2016

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“…31,32 Hydrophilic surfaces have been found favorable for the rapid attachment and spreading of osteoblasts. The improved wettability of surfaces indicates higher surface energies.…”

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Understanding improved osteoblast behavior on select nanoporous anodic alumina

Yan

et al. 2014

IJN

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The aim of this study was to prepare different sized porous anodic alumina (PAA) and examine preosteoblast (MC3T3-E1) attachment and proliferation on such nanoporous surfaces. In this study, PAA with tunable pore sizes (25 nm, 50 nm, and 75 nm) were fabricated by a two-step anodizing procedure in oxalic acid. The surface morphology and elemental composition of PAA were characterized by field emission scanning electron microscopy and X-ray photoelectron spectroscopy analysis. The nanopore arrays on all of the PAA samples were highly regular. X-ray photoelectron spectroscopy analysis suggested that the chemistry of PAA and flat aluminum surfaces were similar. However, contact angles were significantly greater on all of the PAA compared to flat aluminum substrates, which consequently altered protein adsorption profiles. The attachment and proliferation of preosteoblasts were determined for up to 7 days in culture using field emission scanning electron microscopy and a Cell Counting Kit-8. Results showed that nanoporous surfaces did not enhance initial preosteoblast attachment, whereas preosteoblast proliferation dramatically increased when the PAA pore size was either 50 nm or 75 nm compared to all other samples ( P <0.05). Thus, this study showed that one can alter surface energy of aluminum by modifying surface nano-roughness alone (and not changing chemistry) through an anodization process to improve osteoblast density, and, thus, should be further studied as a bioactive interface for orthopedic applications.

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“…However, the possibility of enhancing the bioceramics bioreactivity through their preparation with low temperature methods has been suggested. 18,19 Avoiding the high temperature sintering process (commonly about 1200ºC), nanocrystalline pieces and grains can be prepared. Higher surface area and smaller crystal size could thus provide very interesting bioresponses, especially in SiHA as the osteogenic effect of silicon is mainly explained by its location at the crystal boundaries.…”

Section: Introductionmentioning

confidence: 99%

Effects of nanocrystalline hydroxyapatites on macrophage polarization

et al. 2016

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The present study is focused on the effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nano-SiHA) on the macrophage populations defined as proinflammatory (M1) and reparative (M2) phenotypes. The results demonstrate that both nano-HA and nano-SiHA favour the macrophage polarization towards a M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials.We believe that this work will have an enormous interest for the use of these nanocrystalline hydroxyapatites for bone repair and bone tissue engineering. Silicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their upregulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response triggered after its implantation. Macrophages are the main components of the innate immune system with an important role in healing and tissue remodelling due to their remarkable functional plasticity, existing a whole spectrum of functional populations with varying phenotypic features. The effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nanoSiHA) on the macrophage populations defined as proinflammatory (M1) and reparative (M2) phenotypes, have been evaluated in the present study with RAW 264.7 cells and mouse peritoneal macrophages as in vitro models. With the purpose of favouring the polarization of primary macrophages towards the M1 or M2 phenotype, the pro-inflammatory stimulus LPS or the anti-inflammatory stimulus IL-10 were used respectively, evaluating the biomaterial effects under these conditions. Our results show that both nano-HA and nano-SiHA favour the macrophage polarization towards a M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials designed for bone repair and bone tissue engineering.

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The role of surface wettability and surface charge of electrosprayed nanoapatites on the behaviour of osteoblasts

Cited by 93 publications

References 30 publications

Effects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatites

Effects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatites

Understanding improved osteoblast behavior on select nanoporous anodic alumina

Effects of nanocrystalline hydroxyapatites on macrophage polarization

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