“…- 4) A coordinated conformational change upon ligand coupling of aromatic residues in TM6, also known as CWxP motif around a very conserved tryptophan (6.48) that leads to disruption of the ionic lock and outward movement of TM6 (called Rotamer Toggle Switch), while TM7 undergoes inward movement towards TM5 ( Weinstein, 2006 ; Hofmann et al, 2009 ; Nygaard et al, 2009 ; Holst et al, 2010 ; Standfuss et al, 2011 ; Trzaskowski et al, 2012 ; Valentin-Hansen et al, 2012 ; Tehan et al, 2014 ; Zhang et al, 2015 ; Venkatakrishnan et al, 2016 ; Plazinska et al, 2017 ; Eddy et al, 2018 ; Kaiser et al, 2018 ; Filipek, 2019 ); instead of CWxP, CWLS is found in GPR143 in the same position ( Ghosh et al, 2012 ).
- 5) The PIF motif comprising residues P5.50, I3.40 and F6.44 ( Ballesteros and Weinstein, 1995 ; Ishchenko et al, 2017 ; Schönegge et al, 2017 ; Kato et al, 2019 ; Hilger, 2021 ; Smith, 2021 ); the PIF motif is also not present in GPR143, only P5.50 itself is found.
- 6) The Na + -pocket at a conserved aspartic acid (2.50) ( Liu et al, 2012 ; Yuan et al, 2013 ; Zhang et al, 2013 ; Katritch et al, 2014 ; Eddy et al, 2018 ; Vickery et al, 2018 ; White et al, 2018 ; Ye et al, 2018 ; Chen S. et al, 2019 ; Filipek, 2019 ; Agasid et al, 2021 ); D2.50 important for Na + binding is also present in GPR143.
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