The Role of Storage Lipids in the Relation between Fecundity, Locomotor Activity, and Lifespan of Drosophila melanogaster Longevity-Selected and Control Lines

Moghadam, Neda Nasiri; Holmstrup, Martin; Manenti, Tommaso; Mouridsen, Marie Brandt; Pertoldi, Cino; Loeschcke, Volker

doi:10.1371/journal.pone.0130334

Cited by 22 publications

(11 citation statements)

References 82 publications

(108 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Future studies are needed to compare the nutrient intake of a range of host‐specialist species that also investigate how male reproductive effort is affected. Finally, it has been reported that lipid storage could play a role in the lifespan–reproduction trade‐off (Moghadam et al., 2015). Investigating lipid regulation and their effect on life‐history traits using the GF approach might be a next step to understand the link between aging mechanisms and the degree of specialization.…”

Section: Discussionmentioning

confidence: 99%

Effects of macronutrient intake on the lifespan and fecundity of the marula fruit fly, Ceratitis cosyra (Tephritidae): Extreme lifespan in a host specialist

Malod

Archer

Hunt

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

In insects, lifespan and reproduction are strongly associated with nutrition. The ratio and amount of nutrients individuals consume affect their life expectancy and reproductive investment. The geometric framework (GF) enables us to explore how animals regulate their intake of multiple nutrients simultaneously and determine how these nutrients interact to affect life‐history traits of interest. Studies using the GF on host‐generalist tephritid flies have highlighted trade‐offs between longevity and reproductive effort in females, mediated by the protein‐to‐carbohydrate (P:C) ratio that individuals consume. Here, we tested how P and C intake affect lifespan (LS) in both sexes, and female lifetime (LEP), and daily (DEP) egg production, in Ceratitis cosyra, a host‐specialist tephritid fly. We then determined the P:C ratio that C. cosyra defends when offered a choice of foods. Female LS was optimized at a 0:1 P:C ratio, whereas to maximize their fecundity, females needed to consume a higher P:C ratio (LEP = 1:6 P:C; DEP = 1:2.5 P:C). In males, LS was also optimized at a low P:C ratio of 1:10. However, when given the opportunity to regulate their intake, both sexes actively defended a 1:3 P:C ratio, which is closer to the target for DEP than either LS or LEP. Our results show that female C. cosyra experienced a moderate trade‐off between LS and fecundity. Moreover, the diets that maximized expression of LEP and DEP were of lower P:C ratio than those required for optimal expression of these traits in host‐generalist tephritids or other generalist insects.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of macronutrient intake on the lifespan and fecundity of the marula fruit fly, Ceratitis cosyra (Tephritidae): Extreme lifespan in a host specialist

Malod

Archer

Hunt

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…As many studies have indicated the increased fattening of insects in response to other stress‐induced conditions (Hansen et al., 2013; Moghadam et al., 2015; Piiroinen et al., 2014), we suggest that the increased body fat content in dung beetles from logged forests reinforces the use of this trait as a likely early warning biomarker to assess the environmental quality of disturbed tropical forests.…”

Section: Discussionmentioning

confidence: 99%

“…Reproduction is a high cost process (Hansen et al., 2013), and fat content is considered the currency (Barry & Wilder, 2013). Although not always observed (Flatt et al., 2008; Wilder, Raubenheimer, & Simpson, 2015), the link between reduced reproduction and increased fat storage has been demonstrated for many insects (Hansen et al., 2013; Moghadam et al., 2015; Wilder et al., 2015). Similarly, previous research has found a positive relationship between reduced fecundity, increased fattening, and higher starvation stress resistance (Broughton et al., 2005; Hansen et al., 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Overall, such studies have shown that organisms under stressful conditions exhibit changes in the fat storage (Baldal et al., 2005; Piiroinen et al., 2014), which is suggested as a strategy to increase life span as a result of improved energy consumption efficiency (Broughton et al., 2005; Hansen, Flatt, & Aguilaniu, 2013) and to improve the starvation stress resistance (Arrese & Soulages, 2010; Lee & Jang, 2014). However, changes in the fat metabolism can have negative effects on individual physiological balance (Moghadam et al., 2015; Van Praet et al., 2014), which might reduce the organism fecundity and reproduction (Arrese & Soulages, 2010; Barry & Wilder, 2013; Hansen et al., 2013). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Does selective logging stress tropical forest invertebrates? Using fat stores to examine sublethal responses in dung beetles

França

Barlow

Araújo

et al. 2016

Ecology and Evolution

View full text Add to dashboard Cite

The increased global demand for tropical timber has driven vast expanses of tropical forests to be selectively logged worldwide. While logging impacts on wildlife are predicted to change species distribution and abundance, the underlying physiological responses are poorly understood. Although there is a growing consensus that selective logging impacts on natural populations start with individual stress‐induced sublethal responses, this literature is dominated by investigations conducted with vertebrates from temperate zones. Moreover, the sublethal effects of human‐induced forest disturbance on tropical invertebrates have never been examined. To help address this knowledge gap, we examined the body fat content and relative abundance of three dung beetle species (Coleoptera: Scarabaeinae) with minimum abundance of 40 individuals within each examined treatment level. These were sampled across 34 plots in a before‐after control‐impact design (BACI) in a timber concession area of the Brazilian Amazon. For the first time, we present evidence of logging‐induced physiological stress responses in tropical invertebrates. Selective logging increased the individual levels of fat storage and reduced the relative abundance of two dung beetle species. Given this qualitative similarity, we support the measurement of body fat content as reliable biomarker to assess stress‐induced sublethal effects on dung beetles. Understanding how environmental modification impacts the wildlife has never been more important. Our novel approach provides new insights into the mechanisms through which forest disturbances impose population‐level impacts on tropical invertebrates.

show abstract

“…6A). Although several studies have reported an interaction between lipid stores and longevity in animals [54–59], the mechanisms underlying this effect are not well understood. In flies, genetic induction of β-oxidation rates increases the average lifespan [60].…”

Section: Discussionmentioning

confidence: 99%

Long-chain acyl-CoA synthetase 2 knockdown leads to decreased fatty acid oxidation in fat body and reduced reproductive capacity in the insect Rhodnius prolixus

Auger

Klett

Paula

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

View full text Add to dashboard Cite

Long-chain acyl-CoA esters are important intermediates in lipid metabolism and are synthesized from fatty acids by long-chain acyl-CoA synthetases (ACSL). The hematophagous insect Rhodnius prolixus, a vector of Chagas' disease, produces glycerolipids in the midgut after a blood meal, which are stored as triacylglycerol in the fat body and eggs. We identified twenty acyl-CoA synthetase genes in R. prolixus, two encoding ACSL isoforms (RhoprAcsl1 and RhoprAcsl2). RhoprAcsl1 transcripts increased in posterior midgut on the second day after feeding, and RhoprAcsl2 was highly transcribed on the tenth day. Both enzymes were expressed in Escherichia coli. Recombinant RhoprACSL1 and RhoprACSL2 had broad pH optima (7.5-9.5 and 6.5-9.5, respectively), were inhibited by triacsin C, and were rosiglitazone-insensitive. Both showed similar apparent Km for palmitic and oleic acid (2-6 μM), but different Km for arachidonic acid (0.5 and 6 μM for RhoprACSL1-Flag and RhoprACSL2-Flag, respectively). The knockdown of RhoprAcsl1 did not result in noticeable phenotypes. However, RhoprACSL2 deficient insects exhibited a 2.5-fold increase in triacylglycerol content in the fat body, and 90% decrease in fatty acid β-oxidation. RhoprAcsl2 knockdown also resulted in 20% increase in lifespan, delayed digestion, 30% reduced oviposition, and 50% reduction in egg hatching. Laid eggs and hatched nymphs showed remarkable alterations in morphology. In summary, R. prolixus ACSL isoforms have distinct roles on lipid metabolism. Although RhoprACSL1 functions remain unclear, we propose that RhoprACSL2 is the main contributor for the formation of the intracellular acyl-CoA pool channeled for β-oxidation in the fat body, and is also required for normal reproduction.

show abstract

The Role of Storage Lipids in the Relation between Fecundity, Locomotor Activity, and Lifespan of Drosophila melanogaster Longevity-Selected and Control Lines

Cited by 22 publications

References 82 publications

Effects of macronutrient intake on the lifespan and fecundity of the marula fruit fly, Ceratitis cosyra (Tephritidae): Extreme lifespan in a host specialist

Effects of macronutrient intake on the lifespan and fecundity of the marula fruit fly, Ceratitis cosyra (Tephritidae): Extreme lifespan in a host specialist

Does selective logging stress tropical forest invertebrates? Using fat stores to examine sublethal responses in dung beetles

Long-chain acyl-CoA synthetase 2 knockdown leads to decreased fatty acid oxidation in fat body and reduced reproductive capacity in the insect Rhodnius prolixus

Contact Info

Product

Resources

About