The role of stimulation protocol, number of oocytes retrieved with respect to follicular fluid oxidative stress and IVF outcome

Thaker, Riddhi; Mishra, Vineet; Gor, Mansi; Agarwal, Rohina; Sheth, Hardik Jyotin; Kapadia, Prakruti; Kumar, Sunil

doi:10.1080/14647273.2018.1551630

Cited by 12 publications

(10 citation statements)

References 34 publications

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“…Numerous studies have previously found clear evidences of ROS and RNS overproduction in FF of IF with different clinical pathologies [33][34][35][36], although none of them performed the concomitant evaluation of the full profile of water-and fat-soluble antioxidants. In our group of IF, we found a significant decrease in FF concentrations of ascorbic acid, GSH, α-tocopherol, vitamin A, CoQ 10 , total carotenoids (p < 0.001), and a significant increase of MDA, 8-OH-dG, nitrite, and nitrate (p < 0.001).…”

Section: Discussionmentioning

confidence: 99%

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

Lazzarino

Pallisco²,

Bilotta³

et al. 2021

IJMS

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Nearly 40–50% of infertility problems are estimated to be of female origin. Previous studies dedicated to the analysis of metabolites in follicular fluid (FF) produced contrasting results, although some valuable indexes capable to discriminate control groups (CTRL) from infertile females (IF) and correlate with outcome measures of assisted reproduction techniques were in some instances found. In this study, we analyzed in blind FF of 35 control subjects (CTRL = patients in which inability to obtain pregnancy was exclusively due to a male factor) and 145 IF (affected by: endometriosis, n = 19; polycystic ovary syndrome, n = 14; age-related reduced ovarian reserve, n = 58; reduced ovarian reserve, n = 29; unexplained infertility, n = 14; genetic infertility, n = 11) to determine concentrations of 55 water- and fat-soluble low molecular weight compounds (antioxidants, oxidative/nitrosative stress-related compounds, purines, pyrimidines, energy-related metabolites, and amino acids). Results evidenced that 27/55 of them had significantly different values in IF with respect to those measured in CTRL. The metabolic pattern of these potential biomarkers of infertility was cumulated (in both CTRL and IF) into a Biomarker Score index (incorporating the metabolic anomalies of FF), that fully discriminated CTRL (mean Biomarker Score value = 4.00 ± 2.30) from IF (mean Biomarker Score value = 14.88 ± 3.09, p < 0.001). The Biomarker Score values were significantly higher than those of CTRL in each of the six subgroups of IF. Posterior probability curves and ROC curve indicated that values of the Biomarker Score clustered CTRL and IF into two distinct groups, based on the individual FF metabolic profile. Furthermore, Biomarker Score values correlated with outcome measures of ovarian stimulation, in vitro fertilization, number and quality of blastocysts, clinical pregnancy, and healthy offspring. These results strongly suggest that the biochemical quality of FF deeply influences not only the effectiveness of IVF procedures but also the following embryonic development up to healthy newborns. The targeted metabolomic analysis of FF (using empowered Redox Energy Test) and the subsequent calculation of the Biomarker Score evidenced a set of 27 low molecular weight infertility biomarkers potentially useful in the laboratory managing of female infertility and to predict the success of assisted reproduction techniques.

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Section: Discussionmentioning

confidence: 99%

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

Lazzarino

Pallisco²,

Bilotta³

et al. 2021

IJMS

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“…Lipid peroxidation is the most well-known mechanism underlying cell damage both in plants and animals 21 . Additionally, it is employed as a marker of oxidative stress in cells and tissues.…”

Section: Discussionmentioning

confidence: 99%

Role of total antioxidant capacity and oxidative stress in fertilization and embryo selection in the IVF cycle

Seyfettinoğlu

Şahin

Akdoğan

et al. 2021

Cukurova Medical Journal

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ÖzPurpose: This study aims to evaluate the oxidant and antioxidant parameters in follicular fluid and embryo culture medium in IVF cycles and investigate their effects on embryo quality, fertilization success, and pregnancy. Materials and Methods: Fifty-one patients who underwent IVF/ICSI were included in this prospective study. Two hundred sixty of a total of 454 follicular fluids and the cell culture media of transferred fifty-one embryos (one from each patient) were examined. Antioxidant activity (AOA), Trolox equivalent antioxidant capacity (TEAC), thiobarbiturate reactive substances (TBARS), ferric antioxidant power (FRAP), paraoxonase, nitrotyrosine were analyzed. Results: TBARS were significantly lower in the follicular fluid of fertilized oocytes than in non-fertilized oocytes (21 vs. 29.75 nmol/ml). There was no difference in terms of other parameters. TEAC levels were higher in oocytes in the agonist protocol group than in the antagonist protocol group. In terms of embryo quality, there were no differences between the groups in any analysis. TEAC levels were higher in the embryo cell culture medium in the group that became pregnant after embryo transfer on the second day. Conclusion: When oxidative stress and antioxidant parameters were evaluated in both follicle fluid and embryo cell culture medium, it was determined that lipid peroxidation negatively affected fertilization. However, there is no significant difference in terms of embryo quality and pregnancy rates.Amaç: Bu çalışmada IVF sikluslarında foliküler sıvı ve embriyo kültür ortamındaki oksidan ve antioksidan parametrelerin değerlendirilmesi ve embriyo kalitesi, fertilizasyon başarısı ve gebelik üzerine etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Bu prospektif çalışmaya IVF/ICSI uygulanan 51 hasta dahil edildi. Toplam 454 foliküler sıvıdan iki yüz altmış sıvı ve transfer edilen elli bir embriyonun (her hastadan bir tane) hücre kültürü ortamı incelendi. Antioksidan aktivite (AOA), Trolox eşdeğer antioksidan kapasite (TEAC), tiyobarbitürat reaktif maddeler (TBARS), ferrik antioksidan güç (FRAP), paraoksonaz, nitrotirozin analiz edildi. Bulgular: TBARS, döllenmiş oositlerin foliküler sıvısında, döllenmemiş oositlere göre önemli ölçüde daha düşüktü (21'e karşı 29.75 nmol/ml,). Diğer parametreler açısından fark yoktu. TEAC seviyeleri, agonist protokol grubundaki oositlerde antagonist protokol grubuna göre daha yüksekti. Embriyo kalitesi açısından hiçbir analizde gruplar arasında fark yoktu. İkinci gün embriyo transferinden sonra gebe kalan grupta embriyo hücre kültürü ortamında TEAC düzeyleri daha yüksekti. Sonuç: Hem folikül sıvısında hem de embriyo hücre kültürü ortamında oksidatif stres ve antioksidan parametreler değerlendirildiğinde lipid peroksidasyonunun fertilizasyonu olumsuz etkilediği ancak embriyo kalitesi ve gebelik oranları açısından önemli bir fark olmadığı saptanmıştır.

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“…Although several studies have investigated the impact of oxidative stress in follicular fluid on IVF outcomes, it is hard to draw a definite conclusion because of conflicting results resulting from the use of various markers and IVF outcomes such as live birth, oocyte quality, fertilization rate, and pregnancy rate (Askoxylaki et al, 2013). For example, some studies have reported associations between high follicular fluid levels of TAC and pregnancy and increase in follicle size (Bedaiwy et al, 2012, Gupta et al, 2011, between lipid peroxidation and pregnancy rate (Pasqualotto et al, 2009), between 8-OHdG and oocyte quality (Tamura et al, 2008), between superoxide dismutase and the quality of embryos on the second day of development (Wdowiak, 2015), and between MDA and oocyte retrieval (Thaker et al, 2020). From all these findings, it seems that ROS affects embryo development at different stages.…”

Section: Impact Of Oxidative Stress and Dna Damage On Ivf Outcomesmentioning

confidence: 98%

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

Al‐Saleh

Coşkun

Al-Rouqi

et al. 2021

Reproduction and Fertility

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This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde, 8-hydroxy-2-deoxyguanosine, hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in the sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. Separate multivariate linear regression was used to assess associations between levels of each oxidative stress biomarker and each hormone and smoking parameter (modeled as natural log-transformed). The findings indicate that some oxidative stress and DNA damage biomarkers played a role in disrupting certain reproductive hormones in women and their male partners either by overproducing reactive oxygen species or reducing antioxidant defense capacity. Although women were nonsmokers, COT levels > 50 and 10 µg/L in urine and follicular were observed in 5.7 and 1.7%, respectively. Levels of follicular fluid COT were positively associated with H2O2 and TM. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate > 50%, biochemical pregnancy, clinical pregnancy, and live birth). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that the elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%). The higher antioxidant activity (CAT and TAC) in follicular fluid might positively impact specific IVF outcomes. Lay summary Oxidative stress occurs when antioxidant molecules are insufficient in the body to destroy free radicals that can damage the cells, proteins and DNA, causing different health conditions, including infertility. The role of oxidative stress in female infertility has not received as much attention as male infertility, and research is still limited. This study explored whether the overproduction of free radicals can impact the success of in vitro fertilization (IVF) treatment using several biological markers such as hydrogen peroxide, catalase, and total antioxidant capacity. Our findings revealed that the high antioxidant levels in the fluid surrounding the egg were linked with a high fertilization rate. Additionally, oxidative stress status in couples was associated negatively with several reproductive hormones and smoking status. Biomarkers of oxidative stress and DNA damage might have potential applications in evaluating IVF patients’ clinical characteristics such as causes of infertility, hormonal profile, fertilization rate, implantation and live birth.

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The role of stimulation protocol, number of oocytes retrieved with respect to follicular fluid oxidative stress and IVF outcome

Cited by 12 publications

References 34 publications

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

Role of total antioxidant capacity and oxidative stress in fertilization and embryo selection in the IVF cycle

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

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