The role of sterol carrier protein2 and other hepatic lipid-binding proteins in bile-acid biosynthesis

Lidström-Olsson, Bodil; Wikvall, Kjell

doi:10.1042/bj2380879

Cited by 36 publications

(5 citation statements)

References 37 publications

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“…Sterol carrier protein 2 (SCP2), also called nonspecific lipid transfer protein, is a 13.2 KD base protein and exists in peroxisome, mitochondria, endoplasmic reticulum and cytoplasm [ 2 - 4 ]. As a moderating factor of cholesterol metabolism, it is involved in the biosynthesis of cholesterol [ 5 - 7 ] and the transformation of cholesterol to bile acid [ 8 , 9 ], cholesteryl ester [ 10 ] and sterols [ 11 ]. As a transporting tool, on the other hand, this protein participates in the transportation of cholesterol inside the cell and through the cytoplasm membrane [ 12 , 13 ] as well as the rapid transportation of the newly synthesized cholesterol from endoplasmic reticulum into the bile without the intervention of cytomicrotubule system and Golgi bodies [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Sterol Carrier Protein 2 in Patients with Hereditary Cholesterol Gallstones

Zhao

et al. 2011

BMC Gastroenterol

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BackgroundLithogenic bile is the major cause of cholesterol gallstone, but its pathogenesis is not well understood. The hypersecretion of biliary cholesterol is believed to be an important cause of lithogenic bile. Sterol Carrier Protein 2 (SCP2) participates in cholesterol trafficking and lipid metabolism in hepatocytes and may play a key role in cholesterol gallstone formation.Methods21 cholesterol gallstone genealogies were studied to investigate the expression of SCP2 gene in liver tissue of hereditary and non-hereditary cholesterol gallstone patients as well as non-gallstone patients. The mRNA expression of liver SCP2 in 28 hereditary patients, 30 non-hereditary cholesterol gallstone patients and 32 non-gallstone patients was measured by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The protein expression of liver SCP2 was also detected in all the patients by Western blotting. At the same time, the bile was also analyzed with biochemical techniques and the Cholesterol Saturation Index (CSI) was calculated.ResultsThe mRNA and protein expression of SCP2 was significantly increased in cholesterol gallstone patients compared to those of non-gallstone patients. Moreover, SCP2 was expressed at higher levels in hereditary cholesterol gallstone patients than that of non-hereditary cholesterol gallstone patients. There was significant difference observed in CSI between cholesterol gallstone patients and non-gallstone patients, but not in CSI between hereditary and non-hereditary cholesterol gallstone patients.ConclusionsSCP2 was overexpressed in hereditary cholesterol gallstone patients compared to non-hereditary cholesterol gallstone patients. This finding indicated that SCP2 might be one of the genetic factors contributing to cholesterol gallstone formation, which was always accompanied by the increase of bile lithogenicity.

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Section: Introductionmentioning

confidence: 99%

Overexpression of Sterol Carrier Protein 2 in Patients with Hereditary Cholesterol Gallstones

Zhao

et al. 2011

BMC Gastroenterol

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“…13 Purified SCP-2 stimulated interorganellar cholesterol transfer 14,15 as well as the conversion of free cholesterol into cholesteryl esters, 16,17 steroid hormones, 18 and 7␣-hydroxycholesterol. 19,20 Similarly, SCP-X increased the transfer of sterols among intracellular membranes in vitro. 21 In addi-tion, SCP-X had thiolitic activity on the side chain of cholesterol, suggesting its relevance in bile acid biosynthesis.…”

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confidence: 95%

Sterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice

et al. 2000

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“…Sterol carrier protein 2 (SCP2), famous as non‐specific lipid transfer protein, is a 13.2‐kDa base protein expressed in peroxisome, mitochondria, endoplasmic reticulum, and cytoplasm . The function of SCP2 is involved in the biosynthesis of cholesterol and the transformation of cholesterol to bile acid . Hence, SCP2 plays an essential role in cholesterol metabolism as a moderating factor.…”

Section: Discussionmentioning

confidence: 99%

“…38 The function of SCP2 is involved in the biosynthesis of cholesterol 39,40 and the transformation of cholesterol to bile acid. 41 Hence, SCP2 plays an essential role in cholesterol metabolism as a moderating factor. At present, there has been no direct research on the relationship between SCP2 and tumors.…”

Section: Discussionmentioning

confidence: 99%

Weighted gene coexpression network analysis reveals hub genes involved in cholangiocarcinoma progression and prognosis

Tian¹,

Pu²,

Li³

et al. 2019

Hepatology Research

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AimCholangiocarcinoma (CCA) is a highly malignant tumor found in the bile duct epithelial cells, and the second most common primary tumor of the liver. However, the pivotal roles of molecular biomarkers in oncogenesis of CCA are unclear. Therefore, we aim to explore the underlying mechanisms of progression and screen for novel prognostic biomarkers and treatment targets.MethodThe data of mRNA sequencing and clinical information of CCA patients in The Cancer Genome Atlas was analyzed by weighted gene coexpression network analysis (WGCNA). Modules and clinical traits were constructed according to Pearson's correlation analysis, and Gene Ontology and pathway enrichment analysis were applied. Hub genes of these modules were screened by intramodule analysis; Cytoscape with Search Tool for the Retrieval of Interacting Genes was utilized to visualize protein–protein interaction of these modules; hub genes of these modules were validated afterwards. Furthermore, the significance of these genes was confirmed by survival analysis.ResultsGenes MRPS18A, CST1, and SCP2 were identified as candidate genes in the module, which was associated with clinical traits including pathological stage, histological grade, and liver function and which also affected overall survival of CCA patients. Nineteen hub genes were analyzed together and were associated with progression and prognosis of CCA. Survival analyses found that several of the multiple genes could serve as biomarkers to stratify CCA patients into low‐ and high‐risk groups.ConclusionThese candidate genes could be involved in progression of CCA, which could serve as novel prognostic markers and treatment targets. Moreover, most of them were first reported in CCA and deserve further research.

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The role of sterol carrier protein2 and other hepatic lipid-binding proteins in bile-acid biosynthesis

Cited by 36 publications

References 37 publications

Overexpression of Sterol Carrier Protein 2 in Patients with Hereditary Cholesterol Gallstones

Overexpression of Sterol Carrier Protein 2 in Patients with Hereditary Cholesterol Gallstones

Sterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice

Weighted gene coexpression network analysis reveals hub genes involved in cholangiocarcinoma progression and prognosis

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