The role of starch as transient energy buffer in synchronized microalgal growth in Acutodesmus obliquus

León-Saiki, G. Mitsue; Remmers, Ilse M.; Martens, Dirk E.; Lamers, Packo P.; Wijffels, René H.; Veen, Douwe van der

doi:10.1016/j.algal.2017.05.018

Cited by 41 publications

(69 citation statements)

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“…Knockdown of key genes involved in starch synthesis showed elevated lipid accumulation by redirecting carbon pool toward lipogenesis (Table 1). Since accumulation of starch as an energy storage molecule is not universal in microalgae (León-Saiki et al, 2017), the suppression of lipid catabolism is a more legitimate option to enhance the lipid content irrespective of the microalgal strains. For instance, inhibiting the expression of a multifunctional lipase/phospholipase/acyltransferase enzyme in Thalassiosira pseudonana resulted in increased lipid yields without affecting the growth (Trentacoste et al, 2013).…”

Section: Manipulation Of the Oil Biosynthesis Pathwaymentioning

confidence: 99%

Tailoring Microalgae for Efficient Biofuel Production

et al. 2018

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Depleting fossil fuel, soaring prices, growing demand, and global climate change concerns have driven the research for finding an alternative source of sustainable fuel. Microalgae have emerged as a potential feedstock for biofuel production as many strains accumulate higher amounts of lipid, with faster biomass growth and higher photosynthetic yield than their land plant counterparts. In addition to this, microalgae can be cultured without needing agricultural land or ecological landscapes and offer opportunities for mitigating global climate change, allowing waste water treatment and carbon dioxide sequestration. Despite these benefits, microalgae pose many challenges, including low lipid yield under limiting growth conditions and slow growth in high lipid content strains. Biotechnological interventions can make major advances in strain improvement for the commercial scale production of biofuel. We discuss various strategies, including efficient transformation toolbox, to increase lipid accumulation and its quality through the regulation of key enzymes involved in lipid production, by blocking the competing pathways, pyramiding genes, enabling high cell biomass under nutrient-deprived conditions and other environmental stresses, and controlling the upstream regulators of targets, the transcription factors, and microRNAs. We highlight the opportunities emerging from the current progress in the application of genome editing in microalgae for accelerating the strain improvement program.

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Section: Manipulation Of the Oil Biosynthesis Pathwaymentioning

confidence: 99%

Tailoring Microalgae for Efficient Biofuel Production

et al. 2018

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“…Figure 1. The vertical gray dashed line represents the time point of maximum dilution rate taken from [8]. Dark area represents the dark period.…”

Section: Cluster Functional Analysesmentioning

confidence: 99%

“…An overview of the changes in the transcriptional landscape and experimental measurements of WT through the diurnal LD cycle is presented in Figure 5. [8]. Small and big rectangles indicate that the process is occurring at relatively low or high level, respectively.…”

Section: Temporal Succession Of Cellular Events As Described By the Gmentioning

confidence: 99%

“…The expression profile of processes related to starch synthesis and degradation also show good correlation to the starch measurements ( Figure 5) , again, taking into account a certain delay between expression and appearance of the phenotype. Starch is a transient energy source and is subject to simultaneous synthesis and degradation [8,47], which results in daily fluctuations between net production during the day and consumption during Following cluster 2, the genes in cluster 3 show a peak in expression at 13h. This cluster is enriched for genes involved in the cell cycle and protein maturation.…”

Section: /40mentioning

confidence: 99%

“…L·min -1 air enriched with 2% CO2) were controlled as described by León-Saiki et al [8]. Light was provided in a 16:8h light/dark (LD) block at an incident photon flux density of 500µmol·m -2 ·s -1 (warm white spectrum 450-620 nm).…”

Section: Introductionmentioning

confidence: 99%

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The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Carrères

León-Saiki

Schaap

et al. 2018

Preprint

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Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition.The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during dark period, resulting in early or late time shift for energy demanding processes. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.

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Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty Acids in Microalgae

Khozin‐Goldberg

Sayanova

2019

Grand Challenges in Algae Biotechnology

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The role of starch as transient energy buffer in synchronized microalgal growth in Acutodesmus obliquus

Cited by 41 publications

References 61 publications

Tailoring Microalgae for Efficient Biofuel Production

Tailoring Microalgae for Efficient Biofuel Production

The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty Acids in Microalgae

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