The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review

Hernández-Falcó, Miranda; Sáez‐Espinosa, Paula; López-Botella, Andrea; Aizpurúa, Jon; Gómez‐Torres, María José

doi:10.3390/ijms23073929

Cited by 6 publications

(4 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The correlation of certain sperm RNA markers with litter size, sperm motility before in vitro capacitation, and kinematic parameters underscores the potential role of these genes in sperm function and fertility. Particularly, IZUMO1, which is a protein known to have a role during mammalian sperm-egg fusion ( Hernandez-Falco et al, 2022 ), was linked to motility, capacitation, and fertilization in this study. These data provide new insight that IZUMO1 may have a functional role not only in sperm-egg fusion in mammalian spermatozoa, but also the reliability of other data that genes proven to correlate with sperm functional parameters in the current study are important targets for studying male fertility.…”

Section: Discussionmentioning

confidence: 93%

Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs

Pang,

Park,

Pang

2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Infertility affects a significant percentage of couples worldwide, and male factors contribute significantly to this problem. Traditional assessments of male fertility rely primarily on parameters such as sperm motility, morphology, viability, and concentration. However, these metrics often do not provide a comprehensive understanding of sperm function, which is critical not only for fertilization but also for successful embryo development. Herein, we used porcine spermatozoa as a model to investigate the potential of sperm RNA markers in assessing various aspects of sperm function from motility to fertility. Using artificial insemination, we evaluated male fertility based on the litter size of sows inseminated with sperm from 20 boars. In addition, we measured parameters such as weaning rate, death births, live births, and mummy births. Sperm functional parameters, including motility and kinematics, were assessed before and after in vitro capacitation. Finally, correlations between various sperm functional parameters and sperm RNA markers were explored. Our results revealed interesting relationships between sperm functional parameters. While motility and kinematics were correlated, they were not correlated with sperm capacitation status. Surprisingly, no significant correlations were found between these parameters and male fertility. However, specific parameters of sperm capacitation status after in vitro capacitation were correlated with weaning rate and live births, highlighting their importance in predicting successful fertilization. Further analysis of sperm RNA markers identified genes related to male fertility, including IZUMO1, known for its role in sperm-egg fusion. These genes showed correlations with motility, capacitation, and fertilization parameters, shedding light on their potential roles in sperm function. In conclusion, our study demonstrates that sperm RNA markers hold promise for the diagnosis and prognosis of various aspects of sperm function, providing valuable insights into male infertility. These markers may serve as diagnostic tools to improve our understanding of male fertility problems, ultimately benefiting couples struggling with infertility.

show abstract

Section: Discussionmentioning

confidence: 93%

Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs

Pang,

Park,

Pang

2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, proteins with an MW of 50–65 kDa in spermatozoa could include the IZUMO1 protein located in the acrosomal membrane. IZUMO1 can interact with JUNO proteins found in oocytes, and this interaction is essential for the fertilization process [27] .…”

Section: Resultsmentioning

confidence: 99%

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objective: To determine the correlation between the molecular weight (MW) of proteins in seminal plasma and spermatozoa and the quality of fresh and frozen semen production in Pasundan bulls. Materials and methods: Nine selected Pasundan bulls, aged 5–10 years, from the Regional Artificial Insemination Center at Ciamis, West Java, Indonesia, were used in the study, with fresh semen sperm motility ≥70% and <70%. We analyzed the motility, viability, integrity of the intact plasma membrane (IPM), and the morphological characteristics of spermatozoa. 1D-SDS-PAGE analysis was performed to determine the protein profile by assessing MW, depicted as bands on the gel. Results: The motility, viability, and IPM of spermatozoa had lower values (p < 0.05) in Pasundan bulls named Bagaskara and Kertarajasa compared to the other bulls. Proteins with MW 35–50 kDa were not detected in the seminal plasma of Pasundan bulls, exhibiting low quality in fresh semen. The correlation analysis showed that the non-detected proteins with MW 35–50 kDa in seminal plasma correlated with spermatozoa motility (r = 0.421), viability (r = 0.424), and IPM (r = 0.428) so that fresh semen quality was low in both Pasundan bulls. Analysis of semen volume, spermatozoa concentration, and spermatozoa motility showed that the average frozen semen production of Pasundan bulls per ejaculate was 128.73 ± 15.35 straws. Conclusion: Protein analysis based on MW is a predictive indicator for the quality of fresh semen and the production of frozen semen in Pasundan bulls. Evaluation parameters of fresh semen quality by MW analysis can be used to select Pasundan bulls in Indonesia.

show abstract

“…As in previous reviews published by our research group [10,25], in this systematic review, the same steps were followed. First, generic searches were performed using the Google Scholar portal (https://scholar.google.es/, accessed on 20 November 2021).…”

Section: Search Strategy Heavy Metals In Seminal Plasmamentioning

confidence: 99%

Analytical Determination of Heavy Metals in Human Seminal Plasma—A Systematic Review

López-Botella

Sánchez

Paul³

et al. 2023

Life

Self Cite

View full text Add to dashboard Cite

Infertility is a growing concerning health problem affecting around 15% of couples worldwide. Conventional semen parameters have limited accuracy for male infertility potential determination. Current advances in the understanding of male infertility indicate that environmental and occupational exposure to chemical contaminants are important etiological factors leading to infertility problems. In this context, some heavy metals (HMs) can be considered as endocrine-disrupting compounds (EDCs), thus altering the seminal quality. This systematic review aims to summarize the key points to detect and quantify HMs in human seminal plasma (SP) and the involved analytical tools. Our results showed that that for HM quantification, atomic absorption spectroscopy (AAS) and inductively coupled plasma (ICP) were the most employed techniques while Zn, Cd, Pb, and Cr were the analytes most often detected. Fast, reliable, and sensitive quantification of EDCs in SP could be important for the development of accurate diagnostic and preventive strategies to address male infertility towards providing personalized therapy.

show abstract

The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review

Cited by 6 publications

References 97 publications

Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs

Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Analytical Determination of Heavy Metals in Human Seminal Plasma—A Systematic Review

Contact Info

Product

Resources

About