The Role of Snail in EMT and Tumorigenesis

Wang, Yifan; Shi, Jian; Chai, Kequn; Ying, Xuhua; Zhou, Binhua P.

doi:10.2174/15680096113136660102

Cited by 713 publications

(621 citation statements)

References 113 publications

Supporting

Mentioning

600

Contrasting

Unclassified

Order By: Relevance

“…TGF-β-induced EMT is a fundamental process in organogenesis and cancer progression (14,26). In this process, epithelial cells lose E-cadherin expression while N-cadherin expression is upregulated (2).…”

Section: Discussionmentioning

confidence: 99%

Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β

et al. 2017

View full text Add to dashboard Cite

In tooth root development, periodontal ligament (PDL) and cementum are formed by the coordination with the fragmentation of Hertwig's epithelial root sheath (HERS) and the differentiation of dental follicle mesenchymal cells. However, the function of the dental epithelial cells after HERS fragmentation in the PDL is not fully understood. Here, we found that TGF-β regulated HERS fragmentation via epithelial-mesenchymal transition (EMT), and the fragmented epithelial cells differentiated into PDL fibroblastic cells with expressing of PDL extracellular matrix (ECM). In the histochemical analysis, TGF-β was expressed in odontoblast layer adjacent of HERS during root development. Periostin expression was detected around fragmented epithelial cells on the root surface, but not in HERS. In the experiment using an established mouse HERS cell line (HERS01a), TGF-β1 treatment decreased E-cadherin and relatively increased N-cadherin expression. TGF-β1 treatment in HERS01a induced further expression of important ECM proteins for acellular cementum and PDL development such as fibronectin and periostin. Taken together, activation of TGF-β signaling induces HERS fragmentation through EMT and the fragmented HERS cells contribute to formation of PDL and acellular cementum through periostin and fibronectin expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β

et al. 2017

View full text Add to dashboard Cite

show abstract

“…TWIST overexpression promotes invasion of glioma cells in vitro and in orthotopic glioma xenotransplants in vivo by inducing the expression of EMT-associated genes like MMP-2 and FN-1. The SNAIL family of transcriptional repressors consisting of SNAIL/SNAI1 and SLUG/SNAI2 is known to drive invasion and metastasis in various carcinomas (163). SNAIL binds to E-box DNA sequences of genes related to an epithelial phenotype through carboxy-terminal zinc-finger domains, thereby suppressing their expression.…”

Section: Twist Snail Slug and Zebmentioning

confidence: 99%

Molecular Mechanisms of Glioma Cell Motility

et al. 2017

View full text Add to dashboard Cite

Gliomas are the most common intracranial tumors in humans. The most malignant among these tumors is glioblastoma (GBM), with an incidence of 3-5 out of 100,000 persons in Western countries. GBM arises either de novo (primary GBM) or develops from a lower grade glioma (secondary GBM). The prognosis is poor. GBMs are lethal tumors and even optimal surgical resection, followed by chemotherapy and irradiation, results in a median survival of about 12-15 months. One characteristic that is responsible for GBM malignancy, and its worse prognosis, is the highly infiltrative growth of GBM cells into the healthy brain. GBM cell migration and invasion is a very complex process that is regulated by several factors, which include changes in the migrating cell itself as well as the tumor microenvironment. This chapter provides an overview of routes of invasion of glioma cells, the signaling pathways that drive glioma cell motility, and the processes through which glioma cells modulate their surrounding environment.

show abstract

“…This process is followed by the downregulation of E-cadherin (37), which is considered to mediate homotypic cell-cell adhesion and maintain normal morphology, epithelial cell polarity, and tissue structural integrity by binding to cytosolic β-catenin and forming the E-cadherin/β-catenin complex. β-Catenin is required for the EMT as it binds to E-cadherin and functions as a key molecule in the Wnt signaling pathway (37). The Wnt signaling pathway is crucial for stem cell survival, proliferation, differentiation, and chemo-/radioresistance (37,38) due to the action of Wnt inhibitory factor 1 (WIF-1), a critical inhibitor of the Wnt signaling pathway (39).…”

Section: Discussionmentioning

confidence: 99%

“…β-Catenin is required for the EMT as it binds to E-cadherin and functions as a key molecule in the Wnt signaling pathway (37). The Wnt signaling pathway is crucial for stem cell survival, proliferation, differentiation, and chemo-/radioresistance (37,38) due to the action of Wnt inhibitory factor 1 (WIF-1), a critical inhibitor of the Wnt signaling pathway (39). Several studies have demonstrated that aberrant activation of the Wnt signaling pathway due to genetic and epigenetic alterations occurs in several types of cancer (39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of irradiation on radioresistance, HOTAIR and epithelial-mesenchymal transition/cancer stem cell marker expression in esophageal squamous cell carcinoma

Chen

Liu

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. Radiotherapy is a common therapeutic strategy used to treat esophageal squamous cell carcinoma (ESCC). However, tumor cells often develop radioresistance, thereby reducing treatment efficacy. Here, we aimed to identify the mechanisms through which ESCC cells develop radioresistance and identify associated biomarkers. Eca109 cells were exposed to repeated radiation at 2 Gy/fraction for a total dose of 60 Gy (Eca109R60/2Gy cells). MTT and colony formation assays were performed to measure cell proliferation and compare the radiation biology parameters of Eca109 and Eca109R60/2Gy cells. Cell cycle distributions and apoptosis were assessed by flow cytometry. Reverse transcription-quantitative polymerase chain reaction and western blotting were employed to analyze the expression of HOX transcript antisense RNA (HOTAIR), in addition to biomarkers of the epithelial-mesenchymal transition (EMT) and cancer stem cells (CSCs). Eca109R60/2Gy cells exhibited increased cell proliferation and clone formation, with significantly higher radiobiological parameters compared with the parental Eca109 cells. The Eca109R60/2Gy cells also exhibited significantly decreased accumulation in G 2 phase and increased accumulation in S phase. Additionally, the apoptosis rate was significantly lower in Eca109R60/2Gy cells than in parental Eca109 cells. Finally, HOTAIR expression levels and SNAI1 and β-catenin mRNA and protein expression levels were significantly higher, whereas E-cadherin levels were significantly lower in Eca109R60/2Gy cells than in Eca109 cells. Therefore, our findings demonstrated that radioresistance was affected by the expression of HOTAIR and biomarkers of the EMT and CSCs.

show abstract

The Role of Snail in EMT and Tumorigenesis

Cited by 713 publications

References 113 publications

<b>Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by </b><b>TGF-β </b>

<b>Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by </b><b>TGF-β </b>

Molecular Mechanisms of Glioma Cell Motility

Effects of irradiation on radioresistance, HOTAIR and epithelial-mesenchymal transition/cancer stem cell marker expression in esophageal squamous cell carcinoma

Contact Info

Product

Resources

About