The role of small RNAs in vegetative shoot development

Fouracre, Jim P.; Poethig, R. Scott

doi:10.1016/j.pbi.2015.11.006

Cited by 61 publications

(44 citation statements)

References 123 publications

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“…The interplay of miRNA networks, including miR319 and TCP genes, is a central process in the regulation of leaf development (Chitwood and Sinha, 2016;Rodriguez et al, 2016;Fouracre and Poethig, 2016). To obtain genetic insights into the role of miR319 in the regulation of leaf development, we generated loss-offunction alleles for miR319 and conducted a phenotypic analysis of mir319a/b.…”

Section: Discussionmentioning

confidence: 99%

“…The integration of diverse developmental programs during leaf development requires a complex and robust network of gene regulation. This regulation requires the sophisticated balance between microRNAs (miRNAs) and their target genes (Rodriguez et al, 2016;Fouracre and Poethig, 2016). miR319 and its target genes, TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCP), play pivotal roles in leaf development.…”

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confidence: 99%

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Roles of miR319 and TCP Transcription Factors in Leaf Development

2017

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Sophisticated regulation of gene expression, including microRNAs (miRNAs) and their target genes, is required for leaf differentiation, growth, and senescence. The impact of miR319 and its target TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCP) genes on leaf development has been extensively investigated, but the redundancies of these gene families often interfere with the evaluation of their function and regulation in the developmental context. Here, we present the genetic evidence of the involvement of the MIR319 and TCP gene families in Arabidopsis (Arabidopsis thaliana) leaf development. Single mutations in MIR319A and MIR319B genes moderately inhibited the formation of leaf serrations, whereas double mutations increased the extent of this inhibition and resulted in the formation of smooth leaves. Mutations in MIR319 and gain-of-function mutations in the TCP4 gene conferred resistance against miR319 and impaired the cotyledon boundary and leaf serration formation. These mutations functionally associated with CUP-SHAPED COTYLEDON genes, which regulate the cotyledon boundary and leaf serration formation. In contrast, loss-of-function mutations in miR319-targeted and nontargeted TCP genes cooperatively induced the formation of serrated leaves in addition to changes in the levels of their downstream gene transcript. Taken together, these findings demonstrate that the MIR319 and TCP gene families underlie robust and multilayer control of leaf development. This study also provides a framework toward future researches on redundant miRNAs and transcription factors in Arabidopsis and crop plants.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Roles of miR319 and TCP Transcription Factors in Leaf Development

2017

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“…miRNA-Triggered Decay of Translating mRNAs miRNA-mediated gene silencing regulates key biological processes in plants, including the plasticity of development in the context of the environment (for review, see Ferdous et al, 2015;Fouracre and Poethig, 2016;D'Ario et al, 2017). The fully processed 21-or 22-nucleotide miRNA and its effector ARGONAUTE1 (AGO1) form a core RNA-induced silencing complex (RISC) that controls the fate of specific mRNAs through a mechanism that involves endonucleolytic cleavage and/or translational repression (Rogers and Chen, 2013).…”

Section: Xrn4-mediated Cotranslational Decaymentioning

confidence: 99%

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

2017

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“…In Arabidopsis, the transcription factors, including the mediator complex, the elongator complex, Negative on TATA less 2 (NOT2), and CELL DIVISION CYCLE 5 (CDC5) have been shown to positively regulate MIR transcription through recruiting Pol II to MIR promoters (15)(16)(17). The transcription of some MIRs, such as MIR172 and MIR156, is also subject to physiological, temporal, or spatial regulation by specific transcription factors (18,19). The RNA-binding proteins DAWDLE (DDL) and PLEIOTROPIC REGULATORY LOCUS 1 (PRL1) are proposed to stabilize pri-miRNAs following their transcription (14,20).…”

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confidence: 99%

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Liu

Zhang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis. A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using primiR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5′ arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.

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The role of small RNAs in vegetative shoot development

Cited by 61 publications

References 123 publications

Roles of miR319 and TCP Transcription Factors in Leaf Development

Roles of miR319 and TCP Transcription Factors in Leaf Development

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

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