The role of Smad1/5 in mantle immunity of the pearl oyster Pinctada fucata martensii

Shi, Yu; Pan, Xiaolan; Xu, Meng; Liu, Huiru; Xu, Hanzhi; He, Maoxian

doi:10.1016/j.fsi.2021.04.001

Cited by 11 publications

(3 citation statements)

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“…Lack of effective tools has become a bottleneck for studying gene function in non-model organisms. RNA interference can significantly reduce the expression of target genes both in vivo and in vitro, and it is widely employed for loss-of-function studies in aquaculture animals 23,[32][33][34] . RNAi can be induced in organicism using siRNAs, dsRNAs and shRNAs [35][36][37] .…”

Section: Discussionmentioning

confidence: 99%

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Zhang,

Sun,

et al. 2023

Sci Rep

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The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.

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Section: Discussionmentioning

confidence: 99%

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Zhang,

Sun,

et al. 2023

Sci Rep

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“…Noteworthy is the regulated protein processing by OS and Cb predicted by the KEGG analysis, implying that they work synergistically with GB, in which protein is the predominant component. Additionally, OS also improves the host immunity ( Malhotra and Kang, 2013 ; Shi et al, 2021 ) by increasing SMAD1/5/8 expression ( Ho et al, 2021 ), while the anti-oxidant Cb ( Ramasamy et al, 2014 ) has been reported to protect against gastric ulcers ( Qiu et al, 2020 ) and functions as a nutritional supplementation due to high-soluble amino acid and mineral levels in Cb ( Ramasamy et al, 2014 ). KEGG analysis demonstrated that OS focused on responses to nutrients and hyperoxia, and gastrointestinal functions, including pancreatic secretion, and Cb contributed to re-epithelization by regulating tissue structure homeostasis, which was conducive to the integrity of the endothelial barrier.…”

Section: Discussionmentioning

confidence: 99%

The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

Shao

et al. 2022

Front. Pharmacol.

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Inflammatory bowel disease (IBD) is pathologically characterized by an immune response accommodative insufficiency and dysbiosis accompanied by persistent epithelial barrier dysfunction. The Cao-Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic. The present study was designed to delineate the pharmacological mechanisms of this formula from different aspects of the etiology of ulcerative colitis (UC), a major subtype of IBD. Dextran sodium sulfate (DSS) was given to mice for a week at a concentration of 2%, and the CW solution was administered for 3 weeks. 16S rRNA gene sequencing and untargeted metabolomics were conducted to examine the changes in the microbiome profile, and biochemical experiments were performed to confirm the therapeutic functions predicted by system pharmacology analysis. The CW treatment hampered DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis, which was corroborated by suppressed caspase 3 (Casp3) and interleukin-1b (IL-1b) and increased cleaved caspase 3 expression and casp-3 activity in the colon samples from colitis mice subjected to the CW therapy. Moreover, the CW therapy rescued the decreased richness and diversity, suppressed the potentially pathogenic phenotype of the gut microorganisms, and reversed the altered linoleic acid metabolism and cytochrome P450 activity in murine colitis models. In our in vitro experiments, the CW administration increased the alternative activation of macrophages (Mφs) and inhibited the tumor necrosis factor-α (TNFα)-induced reactive oxygen species (ROS) level and subsequent death in intestinal organoids (IOs). We propose that the CW formula alleviates the progression of murine colitis by suppressing inflammation, promoting mucosal healing, and re-establishing a microbiome profile that favors re-epithelization.

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“…③ IBD patients are susceptible to malnutrition including anemia, amino acid, and vitamin deficiency [ 96 ], which could be rescued by PP [ 97 ] due to its hematopoiesis-promoting effect [ 98 – 100 ] and by the addition of pearl that is produced from shellfish mollusks which contain 20 amino acids and Fe and Mg elements [ 101 ]. ④ Additionally, pearl powder [ 84 , 85 , 102 ] and BT [ 103 ] are conductive to re-epithelization by activating the transforming growth factor (TGF) signaling pathway, and CH alleviates mucosal injury by increasing mucus production and suppressing gastric acid secretion [ 104 , 105 ]. Orchestrating drug metabolism: since most TCM formulas have multiple herbs functioning in a synergistic or counteracting mode to achieve homeostasis, drug interaction is a major challenge for drug efficacy and safety.…”

Section: Discussionmentioning

confidence: 99%

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

Yan

Tang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Inflammatory bowel disease (IBD) is a major cause of morbidity and mortality due to its repetitive remission and relapse. The Jian-Wei-Yu-Yang (JW) formula has a historical application in the clinic to combat gastrointestinal disorders. The investigation aimed to explore the molecular and cellular mechanisms of JW. Methods. 2% dextran sodium sulfate (DSS) was diluted in drinking water and given to mice for 5 days to establish murine models of experimental colitis, and different doses of JW solution were administered for 14 days. Network pharmacology analysis and weighted gene co-expression network analysis (WGCNA) were utilized to predict the therapeutic role of JW against experimental colitis and colitis-associated colorectal cancer (CAC). 16S rRNA sequencing and untargeted metabolomics were conducted using murine feces. Western blotting, immunocytochemistry, and wound healing experiments were performed to confirm the molecular mechanisms. Results. (1) Liquid chromatography with mass spectrometry was utilized to confirm the validity of the JW formula. The high dose of JW treatment markedly attenuated DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis. (2) The JW targets were related to the survival probability in patients with colorectal cancer, underlying a potential therapeutic value in CRC intervention. (3) Moreover, the JW therapy successfully rescued the decreased richness and diversity of microbiota, suppressed the potentially pathogenic phenotype of the gut microorganisms, and increased cytochrome P450 activity in murine colitis models. (4) Our in vitro experiments confirmed that the JW treatment suppressed caspase3-dependent pyroptosis, hypoxia-inducible factor 1α (HIF1α), and interleukin-1b (IL-1b) in the colon; facilitated the alternative activation of macrophages (Mφs); and inhibited tumor necrosis factor-α (TNFα)-induced reactive oxygen species (ROS) level in intestinal organoids (IOs). Conclusion. The JW capsule attenuated the progression of murine colitis by a prompt resolution of inflammation and bloody stool and by re-establishing a microbiome profile that favors re-epithelization and prevents carcinogenesis.

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The role of Smad1/5 in mantle immunity of the pearl oyster Pinctada fucata martensii

Cited by 11 publications

References 50 publications

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

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