The role of SLC transporters for brain health and disease

Yen, Nguyen Thi Hai; Ha, Hoa Thi Thuy; Nguyen, Tra H; Nguyen, Long N.

doi:10.1007/s00018-021-04074-4

Cited by 39 publications

(27 citation statements)

References 225 publications

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“…The comparison of hBMV_NORM with hBMV_PATHO (Supplementary Figure S2) did not reveal any significant differences except for MCT1 and CD147 (respectively 4-fold and 4.6-fold greater in hBMV_PATHO). It has been reported that both proteins are expressed at the BBB level and might be involved in degenerative processes [29,30]. Comparison of the normalized protein expression levels in the isolated human brain microvessels obtained from the two independent donors with no pathology in this study (hBMV_NORM) with the previously reported protein expression levels in the isolated human brain microvessels from seven donors (hBMV_REF) [7].…”

Section: Analysis Of the Protein Expression Levels In Hbmvs And Compa...mentioning

confidence: 72%

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Dehouck

Tachikawa

Hoshi

et al. 2022

Cells

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We previously developed an in vitro model of the human blood–brain barrier (BBB) based on the use of endothelial cells derived from CD34+-hematopoietic stem cells and cultured with brain pericytes. The purpose of the present study was to provide information on the protein expression levels of the transporters, receptors, tight junction/adherence junction molecules, and transporter-associated molecules of human brain-like endothelial cells (hBLECs). The absolute protein expression levels were determined by liquid chromatography–mass spectrometry-based quantitative targeted absolute proteomics and compared with those from human brain microvessels (hBMVs). The protein levels of CD144, CD147, MRP4, Annexin A6 and caveolin-1 showed more than 3-fold abundance in hBLECs, those of MCT1, Connexin 43, TfR1, and claudin-5 showed less than 3-fold differences, and the protein levels of other drug efflux transporters and nutrient transporters were less represented in hBLECs than in hBMVs. It is noteworthy that BCRP was more expressed than MDR1 in hBLECs, as this was the case for hBMVs. These results suggest that transports mediated by MCT1, TfR1, and claudin-5-related tight junction function reflect the in vivo BBB situation. The present study provided a better characterization of hBLECs and clarified the equivalence of the transport characteristics between in vitro BBB models and in vivo BBB models using LC-MS/MS-based protein quantification.

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Section: Analysis Of the Protein Expression Levels In Hbmvs And Compa...mentioning

confidence: 72%

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Dehouck

Tachikawa

Hoshi

et al. 2022

Cells

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“…Moreover, in vivo knockdown of p75NTR with antisense oligonucleotides brought about increased ChAT activity and ACh release in the hippocampus of mature rats [ 128 ]. These data indicate that suppression of the p75NTR signalling pathway may exert a beneficial effect on the outcome of several neurodegenerative diseases through the alleviation of disturbances in energy and acetyl-Co-A metabolism [ 14 , 23 , 129 ].…”

Section: Glucose and Pyruvate-derived Acetyl-coa Metabolism In Cholin...mentioning

confidence: 99%

Metabolic and Cellular Compartments of Acetyl-CoA in the Healthy and Diseased Brain

Jankowska-Kulawy

Klimaszewska-Łata

Gul-Hinc

et al. 2022

IJMS

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The human brain is characterised by the most diverse morphological, metabolic and functional structure among all body tissues. This is due to the existence of diverse neurons secreting various neurotransmitters and mutually modulating their own activity through thousands of pre- and postsynaptic interconnections in each neuron. Astroglial, microglial and oligodendroglial cells and neurons reciprocally regulate the metabolism of key energy substrates, thereby exerting several neuroprotective, neurotoxic and regulatory effects on neuronal viability and neurotransmitter functions. Maintenance of the pool of mitochondrial acetyl-CoA derived from glycolytic glucose metabolism is a key factor for neuronal survival. Thus, acetyl-CoA is regarded as a direct energy precursor through the TCA cycle and respiratory chain, thereby affecting brain cell viability. It is also used for hundreds of acetylation reactions, including N-acetyl aspartate synthesis in neuronal mitochondria, acetylcholine synthesis in cholinergic neurons, as well as divergent acetylations of several proteins, peptides, histones and low-molecular-weight species in all cellular compartments. Therefore, acetyl-CoA should be considered as the central point of metabolism maintaining equilibrium between anabolic and catabolic pathways in the brain. This review presents data supporting this thesis.

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“…Amino acid transport across the placenta is best conceived as a systems approach, in which the concerted effort of accumulative transporters, exchangers, and facilitated transporters with overlapping substrate specificity drive a net transport of amino acids from maternal to fetal circulation (Cleal et al, 2018). Amino acid transporters are also expressed in the fetal brain at the blood-brain barrier, where they facilitate amino acid transport into and out of the fetal brain (Campos-Bedolla et al, 2014), as well as at neuronal synapses, where they help regulate neurotransmission (Nguyen et al, 2022). Regulation of amino acid transport across the placenta and throughout the fetal brain is essential, as amino acids are required for fetal brain development (McDonald and Johnston, 1990;Kurbat and Lelevich, 2009;Tabatabaie et al, 2010;Tochitani, 2017).…”

Section: )mentioning

confidence: 99%

COVID-19 Vaccines and the Virus: Impact on Drug Metabolism and Pharmacokinetics

et al. 2022

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List of non-standard abbreviations: alanine serine cysteine transporter (ASCT), antibody-drug conjugates (ADCs), excitatory amino acid transporter (EAAT), gamma aminobutyric acid (GABA), glycine transporter (GLYT), interferon (IFN), interleukin (IL), mean fluorescence intensity (MFI), nuclear factor kappa B (NF-κB), post-acute covid-19 syndrome (PACS), pegylated (PEG), polyinosinic:polycytidylic acid (Poly(I:C)), small neutral amino acid transporter (SNAT)

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The role of SLC transporters for brain health and disease

Cited by 39 publications

References 225 publications

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Metabolic and Cellular Compartments of Acetyl-CoA in the Healthy and Diseased Brain

COVID-19 Vaccines and the Virus: Impact on Drug Metabolism and Pharmacokinetics

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