The role of serotonin release and autoreceptors in the dorsalis raphe nucleus in the control of serotonin release in the cat caudate nucleus

Becquet, Denis; Faudon, M.; Héry, F.

doi:10.1016/0306-4522(90)90248-3

Cited by 38 publications

(25 citation statements)

References 48 publications

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“…In keeping with previous evidence (Becquet et al, 1990), the application of GABA in the DRN markedly reduced the release of 5-HT in dorsal striatum. Also, the application of R(ϩ)baclofen in the DRN reduced the striatal 5-HT output.…”

Section: Dual Action Of Baclofen On Serotonergic Neuronssupporting

confidence: 88%

“…GABA suppresses the firing activity of serotonergic neurons (Gallagher and Aghajanian, 1976). Consequently, the stimulation of GABA A receptors reduces several neurochemical serotonergic indices in dorsal striatum (Nishikawa and Scatton, 1985;Becquet et al, 1990), a forebrain region innervated exclusively by serotonergic fibers of the DRN (Azmitia and Segal, 1978). Also, GABA inhibits 5-HT synthesis and its release from cultured serotonergic neurons (Becquet et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Abellán¹,

Jolas

Aghajanian

et al. 2000

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We assessed the role of GABA(B) receptors in the control of serotonergic (5-HT) neurons of the dorsal raphe nucleus (DRN) by using microdialysis in vivo and intra- and extracellular recording in vitro in the rat. The GABA(B) agonist R(+)baclofen (but not the inactive S(-)enantiomer) enhanced the 5-HT output in the DRN (4. 7-fold at 15 mg/kg s.c.) and, to a much lesser extent, striatum of unanesthetized rats. Phaclofen (2 mg/kg s.c.) antagonized the effects of 6 mg/kg R(+)baclofen in dorsal striatum. Using dual-probe microdialysis, R(+)baclofen (0.1-100 microM) applied in the DRN enhanced the local 5-HT output (4.5-fold at 100 microM) but decreased that in striatum at 100 microM. At concentrations higher than 100 microM there was a moderate decrement in the elevation of 5-HT in the DRN. In midbrain slices, bath R(+)baclofen exerted a biphasic effect on DRN 5-HT neurons. Consistent with a reduced striatal 5-HT release when infused in the DRN, R(+)baclofen (0.1-30 microM) induced an outward current in 5-HT neurons (IC(50) = 1.4 microM). Lower R(+)baclofen concentrations (0.01-1 microM) preferentially reduced GABAergic inhibitory postsynaptic currents induced by N-methyl-D-aspartate (20 microM) in 5-HT neurons (IC(50) = 72 nM). Using extracellular recordings, R(+)baclofen (300 nM) enhanced the ability of NMDA to induce firing in a subpopulation of serotonergic neurons. These results are consistent with a preferential activation by a low concentration of R(+)baclofen of presynaptic GABA(B) receptors on GABAergic afferents that could disinhibit 5-HT neurons and increase 5-HT release.

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Section: Dual Action Of Baclofen On Serotonergic Neuronssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Abellán¹,

Jolas

Aghajanian

et al. 2000

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“…It has been shown that a decreased serotonergic activity in striatum can be obtained by the activation of 5-HT 1A receptors in DRN. 41,42 Therefore, we explored the possibility that the decreased activity measured after 5-HT injection in serotonergic nerve endings present in major cerebral arteries was due to the activation of the same serotonergic receptor subtype. This seems to be the case, because local delivery of a 5-HT 1A antagonist, WAY-100635, prevented the reduction in serotonergic activity elicited by serotonin in cerebral arteries and striatum.…”

Section: Discussionmentioning

confidence: 99%

Local Treatments of Dorsal Raphe Nucleus Induce Changes in Serotonergic Activity in Rat Major Cerebral Arteries

Marco

Moreno

Pablo

1999

Stroke

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Background and Purpose-Rat major cerebral arteries seem to receive serotonergic fibers originating from the dorsal raphe nucleus (DRN), but little is known about their function. The aim of our present work was to establish a functional relationship between this brain stem nucleus and the cerebral blood vessels by studying the effects of several treatments in the DRN on cerebrovascular serotonergic activity. Methods-Serotonin, clomipramine, 8-OH-DPAT, and WAY-100635 were administered in DRN. A stereotaxically localized electrode allowed the electrical stimulation of this brain stem nucleus. Serotonergic activity was appraised in major cerebral arteries, striatum, and hippocampus from 5-hydroxytryptophan accumulation after aromatic L-amino acid decarboxylase inhibition with NSD-1015. Results-Serotonin significantly decreased serotonergic activity in major cerebral arteries and striatum without affecting it in hippocampus. This reduction was blocked by previous injection of WAY-100635 in DRN. Local administration of 8-OH-DPAT or clomipramine elicited an effect similar to that of serotonin, whereas that of WAY-100635 did not modify serotonergic activity in either of the tissues. Electrical stimulation of DRN significantly increased serotonergic activity in major cerebral arteries and striatum but not in hippocampus. Conclusions-These results confirm the presence of a serotonergic innervation in rat major cerebral arteries functionally related to DRN. 5-HT 1A receptor activation partly mediates the action of serotonin in DRN. A serotonergic tone acting on these somatodendritic receptors was not clearly found. (Stroke. 1999;30:1695-1701.)

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“…More recently we reported in the cat that an increase in 5-HT release within the dorsal raphe nucleus lowered the 5-HT release in the caudate nucleus, i.e., in the terminals and that 5-HT1, receptors located within the dorsal raphe nucleus were involved in this effect (Becquet et al, 1990~). However, these experiments were carried out in the mature CNS.…”

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confidence: 95%

Identification and Role of Serotonin 5‐HT_1A and 5‐HT_1B Receptors in Primary Cultures of Rat Embryonic Rostral Raphe Nucleus Neurons

Peroutka¹

1999

Journal of Neurochemistry

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Autoregulatory mechanisms affecting serotonin [5-hydroxytryptamine (5-HT)] release and synthesis during the early period of development were investigated in dissociated cell cultures raised from embryonic rostral rat rhombencephalon. The presence of 5-HTlA and 5-HT, receptors in serotoninergic neurons was assessed using binding assays. The involvement of 5-HTl A and 5-HTlB receptors in the control of the synthesis and release of PH15-HT was studied using biochemical approaches with several serotoninergic receptor ligands. A mean decrease of 30% in PH15-HT synthesis and release was observed in the presence of 5-HT (lop8 M), the 5-HTl A agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), the 5HTl B/,, agonist 5-methoxy-3-(1,2,5,6-tetrahydr0-4-pyridinyl)-l H-indole (RU 24969), the 5-HTlB agonist 3-(1,2,5,6-tetrahydropyrid-4-yl)pyrrolo 5-HT uptake experiments showed that these two agonists interacted with the 5-HT transporter. 5-HT1 binding sites (620 fmol/mg of protein) and 5-HTlA (482 fmol/mg of protein) and 5-HTlB (1 27 fmol/mg of protein) receptors were detected in 12-day in vitro cell cultures. Experiments carried out with tetrodotoxin suggested that 5-HTlA receptors are located on nerve cell bodies, whereas 5-HTlB receptors are located on the nerve terminals. We concluded that autoregulatory mechanisms Since then, the discovery of multiple 5-HT receptors (Hoyer et al., 1994) has made more difficult the analysis of functions in which central 5-HT systems are involved. Presynaptic 5-HT autoreceptors in the rat brain were initially found to belong to the 5-HT, receptor type (Martin and Sanders-Bush, 1982). Later it became clear that terminal autoreceptors belong to the 5-HT,, subtype (Engel et al., 1986). More recently, many reports indicated that the 5-HTlA receptor subtype is involved in autocontrol at the nerve-cell body level (Mundey et al., 1994). Using anti-5-HTlA receptor antibodies, Sotelo et al. (1990) found that 5-HT,,\ receptors are located solely

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The role of serotonin release and autoreceptors in the dorsalis raphe nucleus in the control of serotonin release in the cat caudate nucleus

Cited by 38 publications

References 48 publications

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Local Treatments of Dorsal Raphe Nucleus Induce Changes in Serotonergic Activity in Rat Major Cerebral Arteries

Identification and Role of Serotonin 5‐HT_1A and 5‐HT_1B Receptors in Primary Cultures of Rat Embryonic Rostral Raphe Nucleus Neurons

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The role of serotonin release and autoreceptors in the dorsalis raphe nucleus in the control of serotonin release in the cat caudate nucleus

Cited by 38 publications

References 48 publications

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Local Treatments of Dorsal Raphe Nucleus Induce Changes in Serotonergic Activity in Rat Major Cerebral Arteries

Identification and Role of Serotonin 5‐HT1A and 5‐HT1B Receptors in Primary Cultures of Rat Embryonic Rostral Raphe Nucleus Neurons

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Product

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Identification and Role of Serotonin 5‐HT_1A and 5‐HT_1B Receptors in Primary Cultures of Rat Embryonic Rostral Raphe Nucleus Neurons