The role of DNA polymerase chain reaction, culture and histopathology in the diagnosis of cutaneous tuberculosis

Abstract

DNA PCR and culture should be advised in cases of cutaneous TB with inconclusive histopathology. DNA PCR should be considered in preference over culture if facilities are available as it gives rapid results and has a higher sensitivity.

A giant mass on the scalp: an unusual presentation of lupus vulgaris

“…However, it is not always possible to identify the exact microorganism with even the most advanced techniques in paucibacillary forms of cutaneous tuberculosis. In a recent study from India, the histopathologic analysis was consistent with LV in all cases ( n = 19), whereas culture was positive in one and PCR was positive in three patients . In problematic cases, a trial of ATT is recommended .…”

An atypical presentation of tuberculous gumma heralding a diagnosis of lymph node tuberculosis: Hindsight is 20/20

“… 5 The diagnosis often requires diagnostic tests other than histology, such as polymerase chain reaction (PCR) and cultures; among the differential diagnosis are scrofuloderma, atypical mycobacterial infections, deep fungal infections, lymphomas, pyogenic infections, and cutaneous leishmaniasis. 6 …”

New Developments in Bacterial, Viral, and Fungal Cutaneous Infections

“…In a study from India, rapid detection via PCR of M. tuberculosis in cutaneous tuberculosis had a sensitivity of 25.8% from samples of histopathologically confirmed cutaneous tuberculosis [66]. In another Indian study, the sensitivity of PCR in detecting cutaneous tuberculosis was 24.5%, which was higher than culture (16.3%) [67]. Thus, PCR can be considered an early diagnostic tool for cutaneous tuberculosis especially when acid-fast bacilli staining and culture results are negative or when fresh material has not been collected for culture.…”