The Role of Ribonucleases in the Immunopathogenesis of Experimental Acute Respiratory Distress Syndrome

Abstract

Background. An adequate assessment of the pathogenesis and course of acute respiratory distress syndrome (ARDS), as well as the search for new methods of treating this pathology, are urgent tasks of modern medicine. The aim of the study was to reveal the influence of ribonuclease enzymes (RNases) on the pathological process of ARDS. Material and methods. Lipopolysaccharide from Escherichia Coli and Pseudomonas Aeruginosa, thromboplastincalcium mixture solution, and recombinant human RNASE2 protein were used to simulate ARDS in laboratory Wistar rats. Results. The resulting models of ARDS were characterized by persistent significant hemostasis disorders and an increase in the level of cytokines; a fragment of human ribonuclease had an impact on the change in the levels of thrombin time, activated partial thromboplastin time and interleukin-6 in the studied models. Conclusions. The changes observed in experimental animals indicate the influence of ribonucleases on the course of the pathological process in ARDS, and the presented models allow us to evaluate the relationship of the selected factors with various outcomes.