The role of riboflavin in decolourisation of Congo red and bioelectricity production using Shewanella oneidensis-MR1 under MFC and non-MFC conditions

Gomaa, Ola M.; Fapetu, Segun; Kyazze, Godfrey; Keshavarz, Tajalli

doi:10.1007/s11274-017-2223-8

Cited by 23 publications

(9 citation statements)

References 22 publications

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“…The results shown in Table indicate that riboflavin was 18.26 mg mL ‐1 for E. coli with insert MtrA , this was followed by that produced by MtrC (12.9 mg mL ‐1 ), and the lowest production was by that containing MtrCAB insert and E. coli (6.3 and 4.2 mg/mL, respectively). These results confirm that riboflavin production is increased as compensation for the lack of MtrC gene, as reported in previous work . Results for CE% followed the order MtrC > MtrA > MtrCAB > WT.…”

Section: Resultssupporting

confidence: 91%

Probing the mechanism of simultaneous bioenergy production and biodegradation process of Congo red in microbial fuel cells

Gomaa

Fapetu

Kyazze

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Many approaches have been employed to increase the understanding and consequently the performance of microbial fuel cells to obtain simultaneous power production and biodegradation. This study uses recombinant Escherichia coli K-12 with MtrA, MtrC and MtrCAB inserts previously prepared using synthetic biology to evaluate the involvement of each of these genes in bioenergy production and biodegradation of Congo red using a double chamber microbial fuel cell. RESULTS:MtrC was the key gene required for energy production corresponding to an average voltage of 360 mV (external resistance 1 k ) and power density of 59 mW m -2 , while E. coli with MtrCAB insert showed the highest decolourisation which reached 80% in 36 h under microbial fuel cell conditions. Coulombic efficiency was 1.2% for E. coli with MtrCAB compared with 2.5% and 2.3% for MtrC and MtrA inserts, respectively. Riboflavin seems to be involved in the electron transferring, its concentration was highest for E. coli with MtrA insert despite its poor performance for both bioenergy production and dye degradation.CONCLUSION: This study suggests that electrons are mutually exclusive between electricity production, dye degradation and other cellular activities. This study helps to improve our understanding of the dual bioenergy/decolourisation process taking place in MFCs in order to maximize the outcome. Electrochemical measurementsVoltage output data were collected using a Picolog ADC-24 (PicoTechnology, UK) online data logging system, Cambridgeshire. Polarisation tests were done by connecting different values of external resistance once voltage had stabilized. Coulombic efficiency (CE) was calculated according to Fernando et al. 14 COD testsThe chemical oxygen demand removal was determined using the closed reflux titrimetric method as described by Westwood. 16 COD was calculated as follows:

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Section: Resultssupporting

confidence: 91%

Probing the mechanism of simultaneous bioenergy production and biodegradation process of Congo red in microbial fuel cells

Gomaa

Fapetu

Kyazze

et al. 2019

J of Chemical Tech & Biotech

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“…The results show that azo reduction is at its best at low nitrate concentrations, whereas high nitrate concentrations affected the biodegradation, this result is in agreement with Cirik et al (2013) who reported an adverse effect on azo dye reduction at high nitrate concentrations. Competition between nitrate and another electron acceptor compound such as azo dyes have been reported (Gomaa et al 2017). The same competition was reported for a co-polluted media containing nitrate and perchlorate suggesting that nitrate has a lower energy barrier for proton and electron transfer (Lv et al 2020).…”

Section: Biophysical Changes Of Bacillus Sp In the Presence Of Different Nitrate Concentrationsmentioning

confidence: 99%

Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation

2021

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The presence of different pollutants in wastewater hinder microbial growth, compromise enzymatic activity or compete for electrons required for bioremediation pathway. Therefore, there is a need to use a single microorganism that is capable of tolerating different toxic compounds and can perform simultaneous bioremediation. In the present study, nitrate reducing bacteria capable of decolorizing azo dye was identi ed as Bacillus subtillis sp. DN using Protein pro ling, morphological and biochemical tests X-ray diffraction pattern, Raman spectroscopy and cyclic voltammetry con rm that the bacterium under study possesses membrane-bound nitrate reductase and that is capable of direct electron transfer. The addition of nitrate concentrations (0-50 mM) resulted in increased bio lm formation with variable exopolysaccharides (EPS), protein, and eDNA. Fourier Transform Infrared spectrum revealed the presence of a biopolymer at high nitrate concentrations. Effective capacitance and conductivity of the cells grown in different nitrate concentrations suggest changes in the relative position of polar groups, their relative orientation and permeability of cell membrane as detected by dielectric spectroscopy. The increase in bio lm shifted the removal of the azo dye from biodegradation to bioadsorption. Our results indicate that nitrate modulates bio lm components. Bacillus sp. DN granular bio lm can be used for simultaneous nitrate and azo dye removal from wastewater.

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“…The anode and the cathode compartments were separated with a cationexchange membrane CMI-7000 (Membranes International USA). The anaerobic anode compartment containing 200 ml working volume was purged with nitrogen gas for 10 min through a 0.22 µm pore size diameter filter prior to inoculation; the media used were MSM prepared according to Gomaa et al (2017), which contained the following (g/L): NH 4 Cl 0.46, KCl 0.225, MgSO 4 .7H 2 O 0.117, NaH 2 PO 4 2.5, Na 2 HPO 4 4.11, (NH 4 ) 2 SO 4 0.225; a vitamin mixture and trace mineral solution were added (1%), and 500 mg/L casein hydrolyzate and 2.2 g/L sodium pyruvate were also added. Cathode chamber contained 200 ml working volume of 100 mM potassium ferricyanide in 50 mM sodium phosphate buffer (pH 7).…”

Section: Enrichment and Characterization Of Anodic Bacteriamentioning

confidence: 99%

“…The use of MFCs in the treatment of textile wastewater has been reported as an alternative approach to classical chemical and physical textile wastewater treatment due to its efficiency and low cost. The decolorization process can increase upon optimizing the operation conditions (Eslami et al, 2019), by adding redox mediators in the anodic chamber (Gomaa, Fapetu, Kyazze, & Keshavarz, 2017) or through incorporating a novel MFC design. During an MFC process, electrons are transferred from the microorganism to the anode, and then they flow through a circuit to the cathode.…”

Section: Introductionmentioning

confidence: 99%

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

Selim

Maghrawy

Fathy

et al. 2020

Journal of Radiation Research and Applied Sciences

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The aim of the present work was to increase bacterial adhesion on anode via inducing membrane modifications to enhance textile wastewater treatment in Microbial Fuel Cell (MFC). Real textile wastewater was used in mediator-less MFCs for bacterial enrichment. The enriched bacteria were pre-treated by exposure to 1 KGy gamma radiation and were tested in MFC setup. Bacterial cell membrane permeability and cell membrane charges were measured using noninvasive dielectric spectroscopy measurements. The results show that pre-treatment using gamma radiation resulted in biofilm formation and increased cell permeability and exopolysaccharide production; this was reflected in both MFC performance (average voltage 554.67 mV) and decolorization (96.42%) as compared to 392.77 mV and 60.76% decolorization for non-treated cells. At the end of MFC operation, cytotoxicity test was performed for treated wastewater using a dermal cell line, the results obtained show a decrease in toxicity from 24.8 to 0 (v/v%) when cells were exposed to gamma radiation. Fourier-transform infrared (FTIR) spectroscopy showed an increase in exopolysaccharides in bacterial consortium exposed to increasing doses of gamma radiation suggesting that gamma radiation increased exopolysaccharide production, providing transient media for electron transfer and contributing to accelerating MFC performance. Modification of bacterial membrane prior to MFC operation can be considered highly effective as a pre-treatment tool that accelerates MFC performance.

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The role of riboflavin in decolourisation of Congo red and bioelectricity production using Shewanella oneidensis-MR1 under MFC and non-MFC conditions

Abstract: Abstract:9 Dissimilatory metal reducing bacteria can exchange electrons extracellularly and hold great 10 promise for their use in simultaneous wastewater treatment and electricity production. This study

Cited by 23 publications

References 22 publications

Probing the mechanism of simultaneous bioenergy production and biodegradation process of Congo red in microbial fuel cells

Probing the mechanism of simultaneous bioenergy production and biodegradation process of Congo red in microbial fuel cells

Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

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