The role of RHOA signaling in trophectoderm cell-fate decision in cattle

Differential regulation of Hippo signaling pathway components between 8‐cell and blastocyst stages of bovine preimplantation embryogenesis

“…Our present findings pertaining to the Cerivastatin inhibition studies, coupled with the results from the mouse model and some limited studies in the bovine model, suggest cell‐cell contact to be an important mediator of Hippo signaling pathway activation, however these results require further investigation (Gumbiner & Kim, 2014; Nishioka et al, 2009; Sharma et al, 2021; Zhao et al, 2007). Third, the absence of significant morphological differences up until the 8‐cell stage of bovine embryogenesis maybe be due to the compensation of the role of this cell signaling pathway components by other related cell signaling pathways (Denicol et al, 2013; Kohri et al, 2020; K.‐B. Lee et al, 2014; Madan et al, 2005).…”

“…These results are similar to that observed in the mouse, where a timely expression of YAP1 in the morulae is essential for lineage segregation (Lorthongpanich et al, 2013). Another possible explanation for the Hippo independent decrease in the gene expression of Yap1 can be due to the regulation of gene expression of Yap1 through other cell signaling pathways, such as the Mevalonate, RHO/ROCK (Rho‐associated kinase) and the Notch signaling pathways, as in the case of mouse (Kohri et al, 2020; Li et al, 2021; Teresa et al, 2014).…”

“…However, the nonpolar cells present inside the blastocyst (ICM cells) turn the pathway “On” resulting in the cytoplasmic retention of p‐YAP1 (Anani et al, 2014; Negron‐Perez & Hansen, 2018; Sharma et al, 2021). In case of bovine embryos, Hippo pathway components, primarily YAP1, Angiomotin (AMOT) and RHOA have been shown to play an important role in TE differentiation and blastocyst formation (Kohri et al, 2020; Negron‐Perez & Hansen, 2018). Significant differences have been observed in the protein localization and expression of p‐YAP1 and SOX2 components, respectively, between the mouse and bovine embryos.…”

A Comparative Analysis of Hippo Signaling Pathway Components during Murine and Bovine Early Mammalian Embryogenesis

“…Although the presence of AMOT has been established in both mouse and bovine embryos, the role of these cell signaling pathway components in bovine embryogenesis and in the subsequent cell fate specification is still unknown [ 33 , 42 ]. In addition to the AMOT polarity protein, RHOA, a small molecule G protein is known to play a significant role in TE differentiation during bovine blastocyst formation [ 43 ]. Specific chemical inhibition of this protein molecule inhibits YAP1, thereby playing an important role in TE differentiation during bovine blastocyst formation [ 43 ].…”

“…In addition to the AMOT polarity protein, RHOA, a small molecule G protein is known to play a significant role in TE differentiation during bovine blastocyst formation [ 43 ]. Specific chemical inhibition of this protein molecule inhibits YAP1, thereby playing an important role in TE differentiation during bovine blastocyst formation [ 43 ].…”