The Role of Rhizotrons and Minirhizotrons in Evaluating the Dynamics of Rhizoplane-Rhizosphere Microflora

McMichael, B. L.; Zak, John C.

doi:10.1007/3-540-29420-1_5

Cited by 2 publications

(3 citation statements)

References 61 publications

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“…The development of methods and equipment such as rhizoboxes has helped to overcome these issues by allowing direct and repeated observations of the roots within the rhizosphere [22,23]. Rhizoboxes also permit the imaging and monitoring of root growth dynamics without disturbance [1,24].…”

Section: Introductionmentioning

confidence: 99%

Variation in the Root System Architecture of Peach × (Peach × Almond) Backcrosses

Lesmes-Vesga

Cano

Ritenour

et al. 2023

Plants

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The spatial arrangement and growth pattern of root systems, defined by the root system architecture (RSA), influences plant productivity and adaptation to soil environments, playing an important role in sustainable horticulture. Florida’s peach production area covers contrasting soil types, making it necessary to identify rootstocks that exhibit soil-type-specific advantageous root traits. In this sense, the wide genetic diversity of the Prunus genus allows the breeding of rootstock genotypes with contrasting root traits. The evaluation of root traits expressed in young seedlings and plantlets facilitates the early selection of desirable phenotypes in rootstock breeding. Plantlets from three peach × (peach × almond) backcross populations were vegetatively propagated and grown in rhizoboxes. These backcross populations were identified as BC1251, BC1256, and BC1260 and studied in a completely randomized design. Scanned images of the entire root systems of the plantlets were analyzed for total root length distribution by diameter classes, root dry weight by depth horizons, root morphological components, structural root parameters, and root spreading angles. The BC1260 progeny presented a shallower root system and lower root growth. Backcross BC1251 progeny exhibited a more vigorous and deeper root system at narrower root angles, potentially allowing it to explore and exploit water and nutrients in deep sandy entisols from the Florida central ridge.

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Section: Introductionmentioning

confidence: 99%

Variation in the Root System Architecture of Peach × (Peach × Almond) Backcrosses

Lesmes-Vesga

Cano

Ritenour

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…This method allows for non-intrusive root observation in a controlled environment. However, it is difficult to apply on large populations and it is best suited for long-term projects as it requires the installment of heavy and expensive equipment (McMichael and Zak, 2006). Also, air gaps forming between the soil and the glass when filling the compartments with soil may affect the growth pattern of the roots.…”

Section: Laboratory and Glasshouse Based Phenotyping Methodsmentioning

confidence: 99%

“…This does not provide a full picture of the entire root system architecture. In addition, air gaps may form at the interface of the soil and inserted tube, affecting root growth; and tubes may also be subject to moisture condensation, hindering the clarity of captured images (McMichael and Zak, 2006). Furthermore, camera lighting systems disturb root growth patterns and can lead to soil temperature increases of up to 3.5 °C (Rewald and Ephrath, 2012).…”

Section: Minirhizotronsmentioning

confidence: 99%

Accelerating the development of durum wheat adapted to drought and crown rot conditions

Alahmad¹

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Global demand for durum wheat (Triticum turgidum L. ssp. durum) will increase with our dramatic continuous growing population. However, production is facing climatic fluctuations and evolving pests and diseases. In Australia, the industry has encountered many challenges surrounding stable and profitable production. These include the lack of reliable rainfall throughout the production regions resulting in severe droughts, and the susceptibility of durum wheat to crown rot (CR; caused predominantly by Fusarium pseudograminearum). Damage to durum wheat crops caused by CR is exacerbated by water stress. Thus far, due to the limited protection attainable by fungicide application and lack of genetic resistance in elite durum breeding germplasm, it has been recognised that breeding varieties incorporating reduced susceptibility to CR would be the most effective control measure when effectively combined with other management practices. For example, improved root system architecture holds some promise for enhancing access to water in arid and semi-arid production areas, particularly in deep soils with high water-holding capacity. This may be advantageous in CR-affected environments if it enables improved access to water that could simultaneously help to avoid drought and so reduce the impact of CR on yield. Hence, this research aimed to identify and combine multiple desirable physiological traits, or adaptive features, to provide some tolerance to CR. Firstly, to identify genetic regions influencing root growth angle (RGA) and CR tolerance, an integrated screening method was developed for phenotyping root variation and CR tolerance, along with other important traits such as leaf rust and plant height in durum wheat. The method was adapted to speed breeding and provided plant breeders with protocols and tools to apply selection in early generations of population development. Therefore, the selection will result in enriching recombinant inbred lines (RILs) with desirable alleles and reduce the number of years required to combine these traits in elite breeding populations. Secondly, a nested association mapping (NAM) population was developed within 18 months using speed breeding technology, including an F4 generation in the field. NAM population were developed by crossing eight elite lines imported from the International Centre for Agricultural Research in the Dry Areas (ICARDA) with two Australian cultivars. This population generated a significant variation for above-and below-ground traits that could be harnessed and explored using genetic mapping and breeding approaches. To identify key genomic regions associated with RGA, a subset of 393 NAM lines were phenotyped using the 'clear pot' method. Combining RGA data with 2,541 polymorphic DArTseq markers information, a major iii independent QTL (qSRA-6A) on 6A chromosome was identified. Furthermore, haplotype analysis for this QTL identified two major haplotype groups in the population, highlighting the opportunity for combining root traits with above-ground traits to better ...

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The Role of Rhizotrons and Minirhizotrons in Evaluating the Dynamics of Rhizoplane-Rhizosphere Microflora

Cited by 2 publications

References 61 publications

Variation in the Root System Architecture of Peach × (Peach × Almond) Backcrosses

Variation in the Root System Architecture of Peach × (Peach × Almond) Backcrosses

Accelerating the development of durum wheat adapted to drought and crown rot conditions

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