The Role of RelA and SpoT on ppGpp Production, Stress Response, Growth Regulation, and Pathogenicity in Xanthomonas campestris pv.
            <i>campestris</i>

Bai, Kaihong; Yan, Huayu; Chen, Xing; Lyu, Qingyang; Jiang, Na; Li, Jianqiang; Luo, Laixin

doi:10.1128/spectrum.02057-21

Cited by 11 publications

(12 citation statements)

References 79 publications

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“…Most of these 11 genes are involved in central metabolic pathways and were also found to be important for growth in artificial media in vitro (Table S2). Nevertheless, we detected (p)ppGpp metabolism as specifically important in xylem sap, suggesting that Xcc faces nutrient scarce conditions that require a stringent response (Irving et al, 2021; Bai et al, 2022).…”

Section: Resultsmentioning

confidence: 83%

“…However, it is interesting to note that the relA gene ( XC_1173 ) involved in (p)ppGpp biosynthesis was only found to be important for growth in xylem sap. Since accumulation of the (p)ppGpp alarmone triggers the stringent response and allows bacterial cells to face nutrient starvation, it is likely that Xcc feeds on anything available in the xylem to ensure multiplication (Bai et al, 2022). In the future, investigating the metabolite composition of guttation fluids and xylem sap will be needed to understand what is available for Xcc in the plant.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide identification of fitness determinants in theXanthomonas campestrisbacterial pathogen during early stages of plant infection

Luneau

Baudin

Quiroz-Monnens

et al. 2022

Preprint

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Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv. campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. 183 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism when only few genes known to be involved in virulence were found to be affected. The biological relevance of 13 genes was independently confirmed by phenotyping single mutants. Notably, we show that the XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study thus revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.

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Section: Resultsmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of fitness determinants in theXanthomonas campestrisbacterial pathogen during early stages of plant infection

Luneau

Baudin

Quiroz-Monnens

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…This is in agreement with the reduced biofilm formation on glass reported for V. cholerae [ 61 ] and Xanthomonas campestris pv. Campestris 8004 [ 122 ] mutants. Similarly, enhanced biofilms on PS were noticed in P. putida KT2440 [ 123 ] and V. alginolyticus [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…and, therefore, facilitating bacterial pathogeny and dissemination [ 124 , 125 ]. Moreover, some authors have related it with the expression of various EPS operons (i.e., vps , pea , peb , bcs ) in diverse bacterial genera, such as Vibrio , Xanthomonas and Pseudomonas ; and, in consequence, with biofilm matrix production [ 26 , 61 , 122 , 123 ]. Moreover, the lack of spoT has also been related to low bacterial growth and no flagella formation, and, consequently, a reduced ability to form biofilms on glass tubes [ 61 , 126 ], which is consistent with our results on reinforced glass.…”

Section: Discussionmentioning

confidence: 99%

Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

et al. 2022

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Arcobacter butzleri, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in A. butzleri. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (flaA, flaB, fliS, luxS, pta and spoT) in the biofilm formation ability of A. butzleri. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in flaAB, fliS and luxS showed a decrease in the biofilm production irrespective of the surface; mutants in spoT showed an increase on stainless steel, and mutants in pta and spoT showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of A. butzleri, although future studies are necessary to achieve a satisfactory objective.

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“…Most of these 11 genes are involved in central metabolic pathways and are important for growth in synthetic media (Table S3). (p)ppGpp metabolism was specifically important in xylem sap, where Xcc probably faces nutrient-scarce conditions requiring a stringent response (Irving et al, 2021;Bai et al, 2022). Before reaching the xylem, Xcc infects hydathodes where it resides as a biotroph for 3 d and becomes a necrotroph at 6 dpi (Cerutti et al, 2017).…”

Section: Hydathode Pores Do Not Impose a Biologically Significant Inf...mentioning

confidence: 99%

Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

et al. 2022

View full text Add to dashboard Cite

Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages.Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media.Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation.This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.

show abstract

The Role of RelA and SpoT on ppGpp Production, Stress Response, Growth Regulation, and Pathogenicity in Xanthomonas campestris pv. campestris

Cited by 11 publications

References 79 publications

Genome-wide identification of fitness determinants in theXanthomonas campestrisbacterial pathogen during early stages of plant infection

Genome-wide identification of fitness determinants in theXanthomonas campestrisbacterial pathogen during early stages of plant infection

Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

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