The role of receptors in prostate cancer

Prinsloo, S.E.; Aswegen, C. H. Van

doi:10.1016/s0065-2423(01)35016-3

Advances in Clinical Chemistry Volume 35

2001

DOI: 10.1016/s0065-2423(01)35016-3

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The role of receptors in prostate cancer

S.E. Prinsloo

C. H. Van Aswegen

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2001

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“…Our study thus aimed to demonstrate the role of Ca 2ϩ pool and SERCA expression in the control of cell proliferation by physiological growth regulators in prostate cancer cells. Androgens are considered to play a crucial role in the development and progression of prostate cancer, since men castrated before puberty do not develop this disease (11). Moreover, antiandrogens inhibit prostate growth in vitro (12) and in vivo (13).…”

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confidence: 99%

Ca2+ Pools and Cell Growth

Legrand¹,

Humez²,

Slomianny³

et al. 2001

Journal of Biological Chemistry

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The present study demonstrates for the first time that intracellular calcium-ATPases and calcium pool content are closely associated with prostate cancer LNCaP cell growth. Cell growth was modulated by changing the amount of epidermal growth factor, serum, and androgene in culture media. Using the microspectrofluorimetric method with Fura-2 and Mag Fura-2 as probes, we show that in these cells, the growth rate is correlated with intracellular calcium pool content. Indeed, an increased growth rate is correlated with an increase in the calcium pool filling state, whereas growth-inhibited cells show a reduced calcium pool load. Using Western blotting and immunocytochemistry, we show that endoplasmic reticulum calcium pump expression is closely linked to LNCaP cell growth, and are a common target of physiological stimuli that control cell growth. Moreover, we clearly demonstrate that inhibition of these pumps, using thapsigargin, inhibits LNCaP cell growth and prevents growth factor from stimulating cell proliferation. Our results thus provide evidence for the essential role of functional endoplasmic reticulum calcium pumps and calcium pool in control of prostate cancer LNCaP cell growth, raising the prospect of new targets for the treatment of prostate cancer.

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confidence: 99%

Ca2+ Pools and Cell Growth

Legrand¹,

Humez²,

Slomianny³

et al. 2001

Journal of Biological Chemistry

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The role of receptors in prostate cancer

Cited by 1 publication

References 255 publications

Ca2+ Pools and Cell Growth

Ca2+ Pools and Cell Growth

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