The role of reactive oxygen species and hemeoxygenase-1 expression in the cytotoxicity, cell cycle alteration and apoptosis of dental pulp cells induced by BisGMA

Chang, Mei‐Chi; Chen, Lin-I; Chan, Chiu‐Po; Lee, Jang‐Jaer; Wang, Tong-Mei; Yang, Ting‐Ting; Lin, Po-Shuen; Lin, Huei‐Jeng; Chang, Hung‐Hao; Jeng, Jiiang‐Huei

doi:10.1016/j.biomaterials.2010.07.049

Cited by 85 publications

(96 citation statements)

References 35 publications

(74 reference statements)

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“…TEGDMA in its unreacted monomer form is relatively hydrophilic and shows greater water solubility potential than most other commonly used monomer components, so that it is more easily eluted from an RBC material in comparison to other monomers like Bis-GMA for example [37]. TEGDMA in particular may have toxic effects on oral tissue cells like gingival or pulp cells [38] and may cause genotoxicity and changes in cytokine expression [39]. Moreover, TEGDMA as its chemical precursor is assumed to be disseminated systemically via salivary-intestinal or pulp-tissue circulation pathways to be metabolized in other inner organs [36].…”

Section: Discussionmentioning

confidence: 98%

Degree of conversion of nano-hybrid resin-based composites with novel and conventional matrix formulation

Frauscher

Ilie

2012

Clin Oral Invest

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Section: Discussionmentioning

confidence: 98%

Degree of conversion of nano-hybrid resin-based composites with novel and conventional matrix formulation

Frauscher

Ilie

2012

Clin Oral Invest

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“…As the CLSM method can sensitively give the initial rate of viable cells, other possibilities could be foreseen to assess more endpoints. In particular, as it has been recently reported that composite monomers affect cells through reactive oxygen species (ROS) [46][47][48] , further investigation is needed to assess correlations if any between cytotoxicity signaling networks and ROS production. The future protocol could be designed to simultaneously evaluate ROS production (H 2 O 2 and/or O 2 -staining) and cytotoxicity ratio (Live/dead staining) using time lapse CLSM imaging.…”

Section: Discussionmentioning

confidence: 99%

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Attik¹,

Gritsch²,

Colon³

et al. 2014

JoVE

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It is generally accepted that in vitro cell material interaction is a useful criterion in the evaluation of dental material biocompatibility. The objective of this study was to use 3D CLSM time lapse confocal imaging to assess the in vitro biocompatibility of dental composites. This method provides an accurate and sensitive indication of viable cell rate in contact with dental composite extracts. The ELS extra low shrinkage, a dental composite used for direct restoration, has been taken as example. In vitro assessment was performed on cultured primary human gingival fibroblast cells using Live/Dead staining. Images were obtained with the FV10i confocal biological inverted system and analyzed with the FV10-ASW 3.1 Software. Image analysis showed a very slight cytotoxicity in the presence of the tested composite after 5 hours of time lapse. A slight decrease of cell viability was shown in contact with the tested composite extracts compared to control cells. The findings highlighted the use of 3D CLSM time lapse imaging as a sensitive method to qualitatively and quantitatively evaluate the biocompatibility behavior of dental composites.

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“…Flow cytometric analysis was performed as described previously [26]. Briefly, EAHY cells (5 x 10 5 cells) were exposed to various concentrations of arecoline for 24 h. Then, both floating and attached cells were collected and centrifuged in tubes.…”

Section: Flow Cytometric Analysis Of Cell Cycle Distribution Of Eahy mentioning

confidence: 99%

Arecoline induced cell cycle arrest, apoptosis, and cytotoxicity to human endothelial cells

Tseng

Chang

Su³

et al. 2011

Clin Oral Invest

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Betel quid (BQ) chewing is a common oral habit in South Asia and Taiwan. BQ consumption may increase the risk of oral squamous cell carcinoma (OSCC), oral submucous fibrosis (OSF), and periodontitis as well as systemic diseases (atherosclerosis, hypertension, etc.). However, little is known about the toxic effect of BQ components on endothelial cells that play important roles for angiogenesis, carcinogenesis, tissue fibrosis, and cardiovascular diseases. EAhy 926 (EAHY) endothelial cells were exposed to arecoline, a major BQ alkaloid, for various time periods. Cytotoxicity was estimated by 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The cell cycle distribution of EAHY cells residing in sub-G0/G1, G0/G1, S-, and G2/M phases was analyzed by propidium iodide staining of cellular DNA content and flow cytometry. Some EAHY cells retracted, became round-shaped in appearance, and even detached from the culture plate after exposure to higher concentrations of arecoline (> 0.4 mM). At concentrations of 0.4 and 0.8 mM, arecoline induced significant cytotoxicity to EAHY cells. At similar concentrations, arecoline induced G2/M cell cycle arrest and increased sub-G0/G1 population, a hallmark of apoptosis. Interestingly, prolonged exposure to arecoline (0.1 mM) for 12 and 21 days significantly suppressed the proliferation of EAHY cells, whereas EAHY cells showed adaptation and survived when exposed to 0.05 mM arecoline. These results suggest that BQ components may contribute to the pathogenesis of OSF and BQ chewing-related cardiovascular diseases via toxicity to oral or systemic endothelial cells, leading to impairment of vascular function. During BQ chewing, endothelial damage may be induced by areca nut components and associate with the pathogenesis of OSF, periodontitis, and cardiovascular diseases.

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The role of reactive oxygen species and hemeoxygenase-1 expression in the cytotoxicity, cell cycle alteration and apoptosis of dental pulp cells induced by BisGMA

Cited by 85 publications

References 35 publications

Degree of conversion of nano-hybrid resin-based composites with novel and conventional matrix formulation

Degree of conversion of nano-hybrid resin-based composites with novel and conventional matrix formulation

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Arecoline induced cell cycle arrest, apoptosis, and cytotoxicity to human endothelial cells

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