The Role of Pseudomonas in Heterotrophic Nitrification: A Case Study on Shrimp Ponds (Litopenaeus vannamei) in Soc Trang Province

Abstract

Based on a total of 6,295,650 sequences from the V3 and V4 regions (16S ribosomal RNA), the composition of the microorganism communities in the water of three Litopenaeus vannamei (Decapoda, Whiteleg shrimp; Soc Trang, Vietnam) ponds were identified. Pseudomonas (10–20.29%), Methylophilus (13.26–24.28%), and Flavobacterium (2.6–19.29%) were the most abundant genera. The total ammonia (TAN) concentration (p = 0.025) and temperature (p = 0.015) were significantly correlated with the relative abundance of Pseudomonas in two bacterial communities (ST1, ST4), whereas the predictive functions of microorganism communities based on 16S rRNA gene data was estimated using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUST), which showed that nitrogen metabolism was significantly negatively correlated (p = 0.049) with TAN concentration. The abundance of Pseudomonas and nitrogen metabolism increased with a decrease in TAN concentration. The correlation between TAN concentration and the abundance of Pseudomonas was followed by the isolation, and heterotrophic nitrifying performance analysis was used to confirm our findings. Six Pseudomonas strains capable of heterotrophic nitrification were isolated from the three water samples and showed a complete reduction of 100 mg/L NH4Cl during a 96-h cultivation. These results indicate the potential of applying Pseudomonas in shrimp ponds for water treatment.

Comparative Genome Analysis of Two Heterotrophic Nitrifying Pseudomonas putida Strains Isolated from Freshwater Shrimp Ponds in Soc Trang Province

“…Additionally, most Pseudomonas putida strains are utilized as non-pathogenic bacteria [28]. TT321 and TT322 have been shown to utilize inorganic nitrogen sources for growth [14]. In this study, genes involved in ammonia conversion and ammonia formation were revealed.…”

“…TT321 and TT322 were isolated from shrimp pond water in Soc Trang province, Vietnam [14]. DNA samples of TT321 and TT322 were extracted using AllPrep ® Bacterial DNA/RNA/Protein Kit (Qiagen, Germantown, MD, USA) following the manufacturer's instructions.…”

“…Pseudomonas putida strains TT321 and TT322 were recently isolated from water samples of shrimp farms in Vietnam. They can remove ammonia via simultaneous heterotrophic nitrification and denitrification in aerobic conditions [14].…”

Effects of the non‐chlorine oxidizer potassium monopersulfate on the water quality, growth performance and microbial community of Pacific white shrimp ( Penaeus vannamei ) culture systems with limited water exchange

“…Adequate levels of water quality parameters are essential condition for the survival of all aquatic organisms’ factors in aquaculture, which is one of the crucial problems restricting the healthy development of aquaculture. (Trung Tran et al, 2019). In the current experiment, the soil placed in the culture tank was taken from the surrounding aquaculture farm.…”

Comparison of the Performance and Microbial Community Structure of Two Outdoor Pilot-Scale Photobioreactors Treating Digestate

“…Another plausible reason is that other microorganisms contributed to the process. For instance, Pseudomonas veronii, which was the dominant bacterial species, might have a role, as it possesses the AmoA gene encoding for the oxygen monooxygenase [67][68][69], and its function as heterotrophic nitrifier was already observed in natural and engineered ecosystems. However, more studies are required to confirm this hypothesis.…”