The Role of Proteomics in Elucidating Multiple Antibiotic Resistance in Salmonella and in Novel Antibacterial Discovery

Pacheco, Ricardo; Correia, Susana; Poeta, Patrícia; Pinto, Luís; Igrejas, Gilberto

doi:10.5772/30599

Cited by 2 publications

(2 citation statements)

References 104 publications

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“…In general, the role of “omics” approaches, integrating genomics, proteomics and transcriptomics are thought to be fundamental in the research for antibiotic/antimicrobial resistance and in the management of microbial resistance [ 31 ]. Notably, proteomics allows one to identify and characterize the proteins from a proteome with the production of useful information, to analyze the expression of proteins and drug targets associated to a disease, and to describe protein interactions, with the final purpose to determine the mechanisms of resistance [ 32 ].…”

Section: Antibiotic/antimicrobial Resistancementioning

confidence: 99%

Antimicrobials from Cnidarians. A New Perspective for Anti-Infective Therapy?

Mariottini

Grice

2016

Marine Drugs

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The ability of microbes to counter the scientific and therapeutic advancements achieved during the second half of the twentieth century to provide effective disease treatments is currently a significant challenge for researchers in biology and medicine. The discovery of antibiotics, and the subsequent development of synthetic antimicrobial compounds, altered our therapeutic approach towards infectious diseases, and improved the quality and length of life for humans and other organisms. The current alarming rise in cases of antibiotic-resistance has forced biomedical researchers to explore new ways to recognize and/or produce new antimicrobials or to find other approaches for existing therapeutics. Aquatic organisms are known to be a source of compounds having the potential to play a role in fighting the battle against pathogenic microbes. In this connection, cnidarians occupy a pre-eminent role. Over the past few decades several studies have explored the antimicrobial/antibiotic properties of cnidarian extracts with the aim of isolating compounds possessing useful therapeutic features. This paper aims to review the existing data on this subject, taking into account the possible utilization of identified compounds.

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Section: Antibiotic/antimicrobial Resistancementioning

confidence: 99%

Antimicrobials from Cnidarians. A New Perspective for Anti-Infective Therapy?

Mariottini

Grice

2016

Marine Drugs

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“…Non-typhoid Salmonella is a common and widely distributed cause of food poisoning [ 1 ]. Even though non-typhoid Salmonella frequently causes self-limited infections, some strains can also cause complicated invasive infections that require antimicrobial therapy [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Complete Proteome of a Quinolone-Resistant Salmonella Typhimurium Phage Type DT104B Clinical Strain

Correia

Nunes-Miranda

Pinto

et al. 2014

IJMS

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Salmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen.

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The Role of Proteomics in Elucidating Multiple Antibiotic Resistance in Salmonella and in Novel Antibacterial Discovery

Cited by 2 publications

References 104 publications

Antimicrobials from Cnidarians. A New Perspective for Anti-Infective Therapy?

Antimicrobials from Cnidarians. A New Perspective for Anti-Infective Therapy?

Complete Proteome of a Quinolone-Resistant Salmonella Typhimurium Phage Type DT104B Clinical Strain

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