The role of poultry and meats in the etiology of Campylobacter jejuni/coli enteritis.

Harris, Noreen V.; Weiss, Noel S.; Nolan, Charles M.

doi:10.2105/ajph.76.4.407

Cited by 245 publications

(119 citation statements)

References 29 publications

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“…In contrast, the organism is a commensal of poultry, from which it enters the human food chain (Harris et al, 1986;Stern et al, 1988;Shane, 1992). In order to reduce the prevalence of C. jejuni in chickens, and thus decrease the risk of human infection, a better understanding of the factors involved in avian colonization is needed.…”

Section: Introductionmentioning

confidence: 99%

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Wassenaar

Zeijst

Ayling³

et al. 1993

Journal of General Microbiology

225

214

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Campylobacter jejuni strain 81 116 contains two flagellin genes, flaA and JlaB. Wild-type (WT) bacteria express JlaA only, but JlaB can be expressed under certain conditions. We have determined the importance of flagella for colonization of the avian caecum, which appears to be the natural environment for these bacteria. Mutants in which JlaA orJlaB, or both had been inactivated, and motility variants, were investigated. Flagella are not a requisite for colonization, but mutants lacking both flagellin genes colonized less efficiently than WT. Inactivation of the JlaB gene, which had no effect on bacterial motility, enhanced chicken caecal colonization 1000-fold compared to WT. A variant (SF-1) with flagella composed of flagellin A, but with poor motility, also colonized better than WT. Conversely, mutants with an inactivatedJEaA gene colonized 100-to 1000-fold less efficiently than WT, regardless of their motility conferred by truncated or full-length flagellin B flagella. These results suggest that the presence of flagellin A, rather than motility, is essential for optimal bacterial colonization of chicken caeca.

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Section: Introductionmentioning

confidence: 99%

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Wassenaar

Zeijst

Ayling³

et al. 1993

Journal of General Microbiology

225

214

View full text Add to dashboard Cite

show abstract

“…Sporadic cases of campylobacteriosis are more common and are associated with the consumption of undercooked chicken. In the United States, case-control studies have attributed 48-70% of sporadic infections to the consumption of Campylobacter-contaminated chickens [11,15]. The percentage of Campylobactercontaminated chicken carcasses varies, often between 50 and 90%, depending on the time of year and the number of carcasses tested.…”

mentioning

confidence: 99%

PCR Detection of Four Virulence-Associated Genes of Campylobacter jejuni Isolates from Thai Broilers and Their Abilities of Adhesion to and Invasion of INT-407 Cells

Chansiripornchai

Sasipreeyajan

2009

The Journal of Veterinary Medical Science

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ABSTRACT. Campylobacter jejuni is a major cause of food borne pathogens in humans and a major reservoir for this pathogen is poultry. The C. jejuni in broilers was investigated from in the caeca of broilers. Twenty broiler/flock samples from 7 flocks were assessed. The average prevalence of C. jejuni was 65% in the broiler flocks. The adhesion and invasion ability of 48 strains of C. jejuni on INT 407 were studied. The adhesion and invasion ability of 48 Campylobacter isolates from caecal contents were analyzed with Human embryonic intestine (INT-407) cells being used as a gentamicin resistance assay. The caecal isolates exhibited a wide range of adherence and invasion ability. There was a significant correlation (p<0.01) between the adherence and the invasion ability of the Campylobacter isolates. Each of the virulence-associated genes: dnaJ, cadF, pldA and ciaB was detected by polymerase chain reaction from 100, 76, 31 and 41% of the Campylobacter strains, respectively. All of four virulence-associated genes were detected in 11 isolates. However, there was unclear association between the invasion ability and the presence of virulence-associated genes in this experiment, suggesting that more genes may be involved in the invasion process.

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“…It is this contamination of meat products that is believed to be the major source of campylobacteriosis in the human population (30). This belief is supported by case-control studies that have identified the consumption of untreated water, unpasteurized milk, and certain meats, often poultry, with the additional risk of campylobacteriosis (31)(32)(33)(34)(35).…”

Section: The Ecology Of Campylobacteriosismentioning

confidence: 96%

Pathogen survival trajectories: an eco-environmental approach to the modeling of human campylobacteriosis ecology.

Skelly

Weinstein²

2003

Environ Health Perspect

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Campylobacteriosis, like many human diseases, has its own ecology in which the propagation of human infection and disease depends on pathogen survival and finding new hosts in order to replicate and sustain the pathogen population. The complexity of this process, a process common to other enteric pathogens, has hampered control efforts. Many unknowns remain, resulting in a poorly understood disease ecology. To provide structure to these unknowns and help direct further research and intervention, we propose an eco-environmental modeling approach for campylobacteriosis. This modeling approach follows the pathogen population as it moves through the environments that define the physical structure of its ecology. In this paper, we term the ecologic processes and environments through which these populations move "pathogen survival trajectories." Although such a modeling approach could have veterinary applications, our emphasis is on human campylobacteriosis and focuses on human exposures to Campylobacter through feces, food, and aquatic environments. The pathogen survival trajectories that lead to human exposure include ecologic filters that limit population size, e.g., cooking food to kill Campylobacter. Environmental factors that influence the size of the pathogen reservoirs include temperature, nutrient availability, and moisture availability during the period of time the pathogen population is moving through the environment between infected and susceptible hosts. We anticipate that the modeling approach proposed here will work symbiotically with traditional epidemiologic and microbiologic research to help guide and evaluate the acquisition of new knowledge about the ecology, eventual intervention, and control of campylobacteriosis.

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The role of poultry and meats in the etiology of Campylobacter jejuni/coli enteritis.

Cited by 245 publications

References 29 publications

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

PCR Detection of Four Virulence-Associated Genes of Campylobacter jejuni Isolates from Thai Broilers and Their Abilities of Adhesion to and Invasion of INT-407 Cells

Pathogen survival trajectories: an eco-environmental approach to the modeling of human campylobacteriosis ecology.

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