The role of polyamines during in vivo and in vitro development

Baron, Kevin; Stasolla, Claudio

doi:10.1007/s11627-008-9176-4

Cited by 100 publications

(52 citation statements)

References 118 publications

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“…For example, recent evidence suggests roles for both Tspm and hydrogen peroxide (H 2 O 2 ) produced from PA catabolism in the control of xylem differentiation (Muñ iz et al, 2008;Tisi et al, 2011). PAs have also been implicated in RNA processing, chromatin remodeling, membrane fluidity, and protein activation (Baron and Stasolla, 2008;Takahashi and Kakehi, 2010). NA, a product of another MTA-generating reaction, is a metal chelator involved in long-distance transport of ions.…”

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confidence: 99%

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

et al. 2012

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5#-Methylthioadenosine (MTA) is the common by-product of polyamine (PA), nicotianamine (NA), and ethylene biosynthesis in Arabidopsis (Arabidopsis thaliana). The methylthiol moiety of MTA is salvaged by 5#-methylthioadenosine nucleosidase (MTN) in a reaction producing methylthioribose (MTR) and adenine. The MTN double mutant, mtn1-1mtn2-1, retains approximately 14% of the MTN enzyme activity present in the wild type and displays a pleiotropic phenotype that includes altered vasculature and impaired fertility. These abnormal traits were associated with increased MTA levels, altered PA profiles, and reduced NA content. Exogenous feeding of PAs partially recovered fertility, whereas NA supplementation improved fertility and also reversed interveinal chlorosis. The analysis of PA synthase crystal structures containing bound MTA suggests that the corresponding enzyme activities are sensitive to available MTA. Mutant plants that expressed either MTN or human methylthioadenosine phosphorylase (which metabolizes MTA without producing MTR) appeared wild type, proving that the abnormal traits of the mutant are due to MTA accumulation rather than reduced MTR. Based on our results, we propose that the key targets affected by increased MTA content are thermospermine synthase activity and spermidinedependent posttranslational modification of eukaryotic initiation factor 5A.

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confidence: 99%

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

et al. 2012

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“…PAs are small polycationic, aliphatic amines capable of electrostatically interacting with macromolecules, such as nucleic acids, phospholipids, cell wall components, and proteins (Baron & Stasolla, 2008). In plants, the three major PAs (putrescine, spermidine and spermine) are synthesized from arginine and ornithine through decarboxylation process (Silveira et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

Almeida

Fraga

Navarro

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.Keywords: orchids, micropropagation, in vitro culture, epigenetics, PLBs.Dinâmica da metilação do DNA global e níveis endógenos de poliaminas durante a indução de estruturas semelhantes a protocormos de Cattleya tigrina A. Richard RESUMO. Cattleya tigrina é uma espécie endêmica do bioma Mata Atlântica e classificada como vulnerável no Livro Vermelho da Flora Brasileira. As técnicas in vitro compreendem ferramentas valiosas a serem empregadas na conservação de espécies de plantas ameaçadas. O objetivo do presente estudo foi avaliar as características morfológicas, os níveis globais de metilação do DNA e as poliaminas livres durante a indução de estruturas semelhantes a protocormos (ESPs). Paralelamente, um protocolo eficiente para a propagação in vitro desta espécie é apresentado. As primeiras evidências de indução de ESPs em C. tigrina foram observadas aos sete dias de cultivo, a partir da porção basal da superfície abaxial da folha. Uma hipometilação foi observada concomitante ao início da diferenciação celular, e um aumento da metilação global do DNA foi encontrada aos 35 dias de cultivo, coincidindo com uma sutil mudança no desenvolvimento morfogenético das estruturas. Durante a indução de ESPs, a putrescina exibiu níveis aumentados em comparação a espermidina e espermina e, aparentemente, apresenta um papel importante durante a indução dessas estruturas em C. tigrina. Devido à aparente formação secundária de ESPs, este protocolo pode representar um método altamente eficiente para a propagação in vitro desta espécie.Palavras-chave: orquideas, micropropagação, cultivo in vitro, epigenética, ESPs.

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“…Polyamines (PAs) are considered reliable markers of metabolic changes that occur during maturation of somatic embryos since they play important roles in plant developmental processes, such as cell division, regulation of morphogenesis, embryogenesis, floral and fruit initiation and development, fruit ripening, leaf senescence, root growth, and tuberization (Baron and Stasolla 2008). In plants, PAs are commonly associated with biotic and abiotic stresses (for review, see Kakkar and Sawhney 2002;Alcázar et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

Cangahuala-Inocente

Silveira

Caprestano

et al. 2013

In Vitro Cell.Dev.Biol.-Plant

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Feijoa (Acca sellowiana [O. Berg] Burret [Myrtaceae]) is a native fruit species of southern Brazil and northern Uruguay. This species is amenable to somatic embryogenesis and therefore suitable as a model system for comparative studies of zygotic and somatic embryo development. In seed plants, embryogenesis involves three main steps, which are regulated by many factors, such as hormones, proteins, polyamines, and transcription factors. In the present work, the dynamics of protein, sugar, starch, amino acid, and polyamine accumulation were assayed during somatic embryogenesis of A. sellowiana . Protein, starch, amino acid, and polyamine levels accumulated unevenly during the induction phase of somatic embryogenesis, while the sugar content remained stable. Throughout the different developmental stages of somatic embryogenesis, synthesis and accumulation of proteins and amino acids showed patterns similar to those reported previously during the development of zygotic embryos of this same species. Differential patterns of polyamine accumulation were observed. This is important because these compounds affect the synthesis of other endogenous growth regulators, such as auxinindole-3-acetic acid that is mainly involved in the establishment of embryo polarity. Taken together, the present work brings new insights to the physiological and biochemical dynamics that occur during somatic embryogenesis of A. sellowiana.

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The role of polyamines during in vivo and in vitro development

Cited by 100 publications

References 118 publications

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

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