The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats

Olorunnisola, Olubukola Sinbad; Adegbola, Peter Ifeoluwa; Ajilore, Bamidele Stephen; Akintola, Olayemi Adebola; Fadahunsi, Olumide Samuel

doi:10.3390/medicines8060025

Cited by 6 publications

(1 citation statement)

References 74 publications

(57 reference statements)

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“…Precise experimental evidence is warranted to ascertain whether the toxic effects of fluoride on VSMCs stem from mitochondrial damage, TCA cycle interference, or other factors. Notably, although the concentrations of sodium on VSMCs cytotoxicity in vivo or in vitro have been reported dozens of times more than we used in this study [27,36,48], the researchers should consider to add sodium control group to tell the different impact between fluoride and sodium administration.…”

Section: Discussionmentioning

confidence: 97%

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

Li,

Yang,

et al. 2024

J Transl Med

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Given the insidious and high-fatality nature of cardiovascular diseases (CVDs), the emergence of fluoride as a newly identified risk factor demands serious consideration alongside traditional risk factors. While vascular smooth muscle cells (VSMCs) play a pivotal role in the progression of CVDs, the toxicological impact of fluoride on VSMCs remains largely uncharted. In this study, we constructed fluorosis model in SD rats and A7R5 aortic smooth muscle cell lines to confirm fluoride impaired VSMCs. Fluoride aggravated the pathological damage of rat aorta in vivo. Then A7R5 were exposed to fluoride with concentration ranging from 0 to 1200 μmol/L over a 24-h period, revealing a dose-dependent inhibition of cell proliferation and migration. The further metabolomic analysis showed alterations in metabolite profiles induced by fluoride exposure, notably decreasing organic acids and lipid molecules level. Additionally, gene network analysis underscored the frequency of fluoride's interference with amino acids metabolism, potentially impacting the tricarboxylic acid (TCA) cycle. Our results also highlighted the ATP-binding cassette (ABC) transporters pathway as a central element in VSMC impairment. Moreover, we observed a dose-dependent increase in osteopontin (OPN) and α-smooth muscle actin (α-SMA) mRNA level and a dose-dependent decrease in ABC subfamily C member 1 (ABCC1) and bestrophin 1 (BEST1) mRNA level. These findings advance our understanding of fluoride as a CVD risk factor and its influence on VSMCs and metabolic pathways, warranting further investigation into this emerging risk factor.

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Section: Discussionmentioning

confidence: 97%

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

Li,

Yang,

et al. 2024

J Transl Med

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Phyllanthus amarus attenuated derangement in renal-cardiac function, redox status, lipid profile and reduced TNF-α, interleukins-2, 6 and 8 in high salt diet fed rats

Olorunnisola

Fadahunsi

Adegbola

et al. 2021

Heliyon

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High salt diet (HSD) has been implicated in the etiopathogenesis of immune derangement, cardiovascular disorders and, metabolic syndromes. This study investigated the protective effect of ethanol extract of Phyllanthus amarus (EEPA) against high salt diet (HSD) induced biochemical and metabolic derangement in male Wistar rats. The rats were divided into 5 groups of 6 animals each as follows; control group fed with normal rat chow, negative control group, fed HSD only, animals on HSD treated orally with 75 mg/kg, 100 mg/kg, and, 150 mg/kg EEPA once daily. At the end of 8 weeks treatment, lipid profile (TG, TC, LDL, and VLDL), oxidative stress (catalase, reduced glutathione, and malondialdehyde), inflammatory (TNF-a, interleukins 2, 6, and 8), cardiac (lactate dehydrogenase, creatine kinase) and kidney function markers (urea, uric acid, creatinine) were assessed. Serum TG, TC, LDL, and VLDL content were significantly (p < 0.05) elevated in HSD-only fed rats, while HDL was significantly elevated in a concentration-dependent manner in EEPA treated animals. The extract produced a significant (p < 0.05) and dose-dependent increase in the antioxidant enzymes activities and a significant reduction in the malondialdehyde level. A significant (p < 0.05) dose-dependent reduction in serum TNF-alpha, IL-2, 6, and 8 of EEPA treated rats compared with HSD-fed rats was observed. More so, reduction in serum LDH, creatine kinase, creatinine, urea, and uric acid activity of extract-treated animals were noted. EEPA attenuated high salt diet-induced oxidative stress, inflammation, and dyslipidemia in rats.

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Identification of two kinds of Colchicum corms by DNA barcoding, physicochemical, chromatographic and chemometric analyses along with pharmacognostic parameters

Gandhi,

Rawat,

Kumar

et al. 2023

Microchemical Journal

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The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats

Cited by 6 publications

References 74 publications

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

Phyllanthus amarus attenuated derangement in renal-cardiac function, redox status, lipid profile and reduced TNF-α, interleukins-2, 6 and 8 in high salt diet fed rats

Identification of two kinds of Colchicum corms by DNA barcoding, physicochemical, chromatographic and chemometric analyses along with pharmacognostic parameters

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