The Role of Podocalyxin in Health and Disease

Nielsen, Julie S.; McNagny, Kelly M.

doi:10.1681/asn.2008070782

Cited by 194 publications

(194 citation statements)

References 66 publications

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“…The expression of SLC2A1 is regulated by the VHL/HIF1 pathway and associated with the progression of renal cell carcinoma [25], indicating another hypothetical role of PLAGL1 transcriptional activity under pathological conditions. The presence of PLAGL1 protein in the renal corpuscles could be also associated with the regulation of the expression PODXL and COL4A3 genes which protein products, podocalyxin and collagen type IV, play a key role in the maintaining and functioning of the renal filtration barrier [26,27]. Taken together, these findings could explain the ubiquitous renal immunoreactivity of the PLAGL1 protein observed in the present study.…”

Section: Pth1rsupporting

confidence: 75%

PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney

Godlewski

Krazinski

Kieżun

et al. 2015

Folia Histochem Cytobiol.

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Introduction. PLAGL1 (pleiomorphic adenoma gene-like 1) is a C2H2-type zinc finger transcription factor associated with the regulation of cell growth and development. Although PLAGL1 expression in kidney was assessed by biochemical methods, the exact localization of the PLAGL1 protein in human kidney has not yet been described. Material and methods. Macroscopically unchanged specimens of kidney tissue were collected from 39 patients undergoing nephrectomy due to renal cell carcinoma. H & E staining of paraffin sections was used to assess histology of the kidney whereas immunohistochemistry was used to localize PLAGL1 protein in kidney compartments. In addition, database sequences search for putative PLAGL1 binding sites among the kidney-related genes was performed. Results. PLAGL1 staining intensity differed depending on the kidney compartment. Strong PLAGL1 immunoreactivity was found in thick ascending limbs of Henle's loop, distal tubules and collecting ducts, whereas PLAGL1 expression in proximal tubules and renal corpuscles (including podocytes) was moderate and weak, respectively. By the in sillico screening of promoter sequences for PLAGL1 specific DNA-binding sites GGG-GCCCC we designated 43 candidate genes for PLAGL1-regulated genes. Analysis of their functional annotations identified three significantly over-represented gene sets: inositol phosphate metabolic processes (GO), endocrine and other factor-regulated calcium reabsorption (KEGG) and calcium signaling pathways (KEGG). Conclusion. Differences in the renal expression of PLAGL1 suggest that this protein may be involved in the regulation of several cellular pathways both as transcriptional factor and coactivator/corepressor of other transcription factors reflecting its role in the cell type-specific control

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Section: Pth1rsupporting

confidence: 75%

PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney

Godlewski

Krazinski

Kieżun

et al. 2015

Folia Histochem Cytobiol.

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“…In addition, PDX has also been observed in subsets of breast, prostate, liver, pancreatic and kidney cancer, as well as leukemia. 15 PDX is likely the most prevalently applied marker protein for podocyte diagnostics in the urine. It is a 140 kDa polyanionic sialoprotein and is localized to plasma membrane of podocytes.…”

Section: Function Of Podocytes In Kidney Diseasesmentioning

confidence: 99%

Relationship between Urinary Podocytes and Kidney Diseases

Sun

Zhao

Li³

2012

Renal Failure

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Podocyte loss is an important component of disease progression in glomerular diseases. To some extent, the loss of podocytes can predict the degree of damage and the advancement of renal disease. Detecting the loss of podocytes in the urine could be a valuable, noninvasive method for obtaining information about the activity of the disease or the disease type, allowing the early diagnosis of glomerular diseases. One of the most robust markers that has been successfully used for urinary podocyte diagnostics is podocalyxin (PDX). PDX is a sialoprotein that is expressed on podocytes and on a variety of nonrenal cells as well as on glomerular endothelial and parietal epithelial cells. Therefore, podocyte loss can be detected by the amount of PDX in the urine. The relationship between urinary podocytes and renal diseases is supported by the detection of podocytes in patients with immunoglobulin A (IgA) nephropathy, HenochSchönlein purpura nephritis, lupus nephritis, diabetic nephropathy, and focal segmental glomerulosclerosis. The use of technology for detecting podocytes in the urine would have broad implications for the evaluation of disease activity, the degree of dedifferentiation, and the possibility of regeneration.

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“…Micro-magnetic-beads made of ferromagnetic material covered with antibodies which also specifically react with the target marker, are then mixed into the solution. Thus, if the specimen includes the target marker, the micro-magnetic-beads are fixed on the device using the marker (antigen) as a "bonding" material (using the sandwich method [7]) (Fig. 2 (b)).…”

Section: Device Structure and Inspection Principlementioning

confidence: 99%