The Role of Plasma Membrane Pleiotropic Drug Resistance Transporters in the Killer Activity of Debaryomyces hansenii and Wickerhamomyces anomalus Toxins

Czarnecka, Monika; Połomska, Xymena; Restuccia, Cristina; Żarowska, Barbara

doi:10.3390/toxins14030180

Cited by 3 publications

(3 citation statements)

References 56 publications

(82 reference statements)

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“…The FY1679-28C (MATa, ura3-52, leu2-D1, trp1-D63, his3-D200, GAL2+) strain was used as the wild-type strain [45][46][47][48][49]. To construct its derivatives with single or double gene deletions, the open reading frames of UME6, PDR3, or RPD3 were replaced with KanMX or bleMX6 gene cassettes by PCR-mediated one-step gene disruption in the FY1679-28C background [45,46,49].…”

Section: Yeast Strains Plasmids and Mediamentioning

confidence: 99%

In Saccharomyces cerevisiae ρ0 Cells, UME6 Contributes to the Activation of ABC Transporter Genes and Pleiotropic Drug Resistance via RPD3 and PDR3

Funasaka,

Ota,

Yamada

2024

Microbiology Research

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In Saccharomyces cerevisiae, the Rpd3L complex includes the histone deacetylase Rpd3 and the DNA binding proteins Ume6 and Ash1 and serves as a transcriptional silencer or enhancer. In S. cerevisiae, the transcription of PDR5, which encodes a major drug efflux pump, and pleiotropic drug resistance (PDR) are hyperactivated by the transcription factor Pdr3 in ρ0/− cells, which lack mitochondrial DNA. We previously showed that RPD3 and UME6 are required for the activation of PDR5 transcription and PDR in S. cerevisiae ρ0 cells. Here, using real-time PCR analysis, we revealed that RPD3 and UME6 are responsible for the activated basal expression of the ABC transporter-encoding genes SNQ2, PDR15, and PDR5 in S. cerevisiae ρ0 cells. Furthermore, using real-time PCR analysis and a spot dilution assay, we found that Ume6 increases the basal expression of PDR5 and PDR15 and induces PDR in a manner dependent on RPD3 and PDR3 in ρ0 cells. This finding may contribute to the elucidation of the relationships between the molecules required for the activation of ABC transporter genes in S. cerevisiae ρ0/− cells and in pathogenic Candida species.

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Section: Yeast Strains Plasmids and Mediamentioning

confidence: 99%

In Saccharomyces cerevisiae ρ0 Cells, UME6 Contributes to the Activation of ABC Transporter Genes and Pleiotropic Drug Resistance via RPD3 and PDR3

Funasaka,

Ota,

Yamada

2024

Microbiology Research

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“…Among BCAs, several yeasts have demonstrated antagonistic activity against different fungal pathogens of fruits and vegetables [ 9 , 10 ], with several mechanisms of action [ 11 , 12 , 13 , 14 , 15 , 16 ]. Among the yeast species, Metschnikowia spp., Aureobasidium spp.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Efficacy of Metschnikowia spp. Yeasts in Organic Vineyards against Major Airborne Diseases of Table Grapes in the Field and in Postharvest

Lombardo,

Panebianco,

Restuccia

et al. 2023

Foods

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The aim of this work was to evaluate the efficacy of two antagonistic yeasts, Metschnikowia pulcherrima strain MPR3 and M. fructicola strain NRRL Y-27328 (commercial product NOLI), applied in addition to the “on-farm biological treatments” (BIO) carried out during the production season, for the containment of powdery mildew and grey mould diseases on organic table grapes ‘Italia’. The yeast strains were applied in the field three times, and their efficacy was evaluated during the production season and under postharvest conditions. Overall, M. pulcherrima MPR3 combined with BIO treatments reduced disease incidence caused by Erysiphe necator and disease incidence and severity caused by Botrytis cinerea with values between 67.8% and 86.2%, showing higher efficacy than BIO treatments applied alone and in combination with NOLI. Field treatments based on BIO+MPR3 maintained their performance also during fruit storage, protecting grape berries from grey mould development to a greater extent than the other treatments (disease reduction of about 98%). Thus, the presence of M. pulcherrima MPR3 seems to improve disease management both in the field and in postharvest environments, without negative impacts on grape microbial communities. These findings highlight the potential of M. pulcherrima MPR3 as a promising alternative strategy for disease control in organic vineyards and in postharvest, providing sustainable solutions to improve food quality and safety.

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“…They showed that the Snq2p efflux pump influences the immunity to the W. anomalus BS91 killer toxin and that the activity of the killer toxins of D. hansenii AII4b, KI2a, MI1a and CBS767 strains is regulated by other transporters than those influencing the W. anomalus killer toxin activity. This is the first study that reports the involvement of PDR transporters in the cell membrane of susceptible microorganisms in resistance to killer yeasts’ toxins [ 7 ].…”

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confidence: 99%

Editorial of the Special Issue “Toxins: Mr Hyde or Dr Jekyll?”

Ladant

Prévost²,

Popoff

et al. 2023

Toxins

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The Role of Plasma Membrane Pleiotropic Drug Resistance Transporters in the Killer Activity of Debaryomyces hansenii and Wickerhamomyces anomalus Toxins

Cited by 3 publications

References 56 publications

In Saccharomyces cerevisiae ρ0 Cells, UME6 Contributes to the Activation of ABC Transporter Genes and Pleiotropic Drug Resistance via RPD3 and PDR3

In Saccharomyces cerevisiae ρ0 Cells, UME6 Contributes to the Activation of ABC Transporter Genes and Pleiotropic Drug Resistance via RPD3 and PDR3

Biocontrol Efficacy of Metschnikowia spp. Yeasts in Organic Vineyards against Major Airborne Diseases of Table Grapes in the Field and in Postharvest

Editorial of the Special Issue “Toxins: Mr Hyde or Dr Jekyll?”

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