The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae

Barraza, Carla Eliana; Solari, Clara A.; Marcovich, Irina; Kershaw, Christopher J.; Galello, Fiorella; Rossi, Silvia; Ashe, Mark P.; Portela, Paula

doi:10.1371/journal.pone.0185416

Cited by 20 publications

(29 citation statements)

References 54 publications

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“…TORC1 (target of rapamycin complex 1) is recruited to SGs under robust HS, which causes its inhibition [ 54 ]. Catalytic subunits of protein kinase A, Tpk2 and Tpk3, have also been identified in HS SGs [ 55 ]. Recently, the recruitment of Cdc28/Cdk1 to SGs by Whi8 has been reported with a function for Cdk1 in SGs disassembly [ 56 ].…”

Section: Introductionmentioning

confidence: 99%

eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules

Malcová

Senohrábková

Nováková

et al. 2021

IJMS

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Stress granules (SGs) are membrane-less assemblies arising upon various stresses in eukaryotic cells. They sequester mRNAs and proteins from stressful conditions and modulate gene expression to enable cells to resume translation and growth after stress relief. SGs containing the translation initiation factor eIF3a/Rpg1 arise in yeast cells upon robust heat shock (HS) at 46 °C only. We demonstrate that the destabilization of Rpg1 within the PCI domain in the Rpg1-3 variant leads to SGs assembly already at moderate HS at 42 °C. These are bona fide SGs arising upon translation arrest containing mRNAs, which are components of the translation machinery, and associating with P-bodies. HS SGs associate with endoplasmatic reticulum and mitochondria and their contact sites ERMES. Although Rpg1-3-labeled SGs arise at a lower temperature, their disassembly is delayed after HS at 46 °C. Remarkably, the delayed disassembly of HS SGs after the robust HS is reversed by TDP-43, which is a human protein connected with amyotrophic lateral sclerosis. TDP-43 colocalizes with HS SGs in yeast cells and facilitates cell regrowth after the stress relief. Based on our results, we propose yeast HS SGs labeled by Rpg1 and its variants as a novel model system to study functions of TDP-43 in stress granules disassembly.

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Section: Introductionmentioning

confidence: 99%

eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules

Malcová

Senohrábková

Nováková

et al. 2021

IJMS

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“…Yeast cells are known to respond at the level of translation to a multitude of environmental stresses, such as heat shock [11,12], nutrient deprivation [13,14,15], general amino acid balance [16,17,18,19], phosphate [20], sulfur [21,22], nitrogen [23,24] content and other environmental conditions [25,26], such as growth at low/high temperatures [27], in different oxygen levels [28,29,30] or in the presence of heavy metals, during desiccation [31,32,33], etc. Many of these conditions are studied in the context of industrial applications of yeast in bread baking and alcohol fermentation [34,35,36,37,38].…”

Section: Introductionmentioning

confidence: 99%

Control of Translation at the Initiation Phase During Glucose Starvation in Yeast

Janapala

Preiß

Shirokikh

2019

IJMS

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Glucose is one of the most important sources of carbon across all life. Glucose starvation is a key stress relevant to all eukaryotic cells. Glucose starvation responses have important implications in diseases, such as diabetes and cancer. In yeast, glucose starvation causes rapid and dramatic effects on the synthesis of proteins (mRNA translation). Response to glucose deficiency targets the initiation phase of translation by different mechanisms and with diverse dynamics. Concomitantly, translationally repressed mRNAs and components of the protein synthesis machinery may enter a variety of cytoplasmic foci, which also form with variable kinetics and may store or degrade mRNA. Much progress has been made in understanding these processes in the last decade, including with the use of high-throughput/omics methods of RNA and RNA:protein detection. This review dissects the current knowledge of yeast reactions to glucose starvation systematized by the stage of translation initiation, with the focus on rapid responses. We provide parallels to mechanisms found in higher eukaryotes, such as metazoans, for the most critical responses, and point out major remaining gaps in knowledge and possible future directions of research on translational responses to glucose starvation.

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“…The distribution of HSP30 and ENO2 mRNAs, whose translation is upregulated upon heat shock, in polysome or sub-polysome fractions was also evaluated. Hsp30 is a negative regulator of the H (+)-ATPase Pma1p and a stress-responsive protein induced by heat shock (Barraza et al, 2017). The HSP30 mRNA amount in the polysome fraction increased upon heat stress (Fig.…”

Section: Tpk1 Mrna Granules In Heat-stressed Cells Can Be Associated With Repressed Translationmentioning

confidence: 98%

“…4C, right panel). ENO2 encodes the enolase II protein and its translational activity decreased upon heat stress (Barraza et al, 2017)(Fig. 4C, bottom panel).…”

Section: Tpk1 Mrna Granules In Heat-stressed Cells Can Be Associated With Repressed Translationmentioning

confidence: 99%

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

Canononero

Pautasso

Galello

et al. 2021

Preprint

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In Saccharomyces cerevisiae, cAMP regulates a number of different cellular processes, such as cell growth, metabolism, stress resistance and gene transcription. The intracellular target for this second messenger in yeast cells is the cAMP-dependent protein kinase (PKA). The way in which a broad specificity protein kinase mediates one right physiological response after cAMP increase indicates that specificity is highly regulated in the cAMP / PKA system. Here we address the mechanism through which cAMP-PKA signalling mediates its response to heat shock thermotolerance in Saccharomyces cerevisiae. Yeast PKA is a tetrameric holoenzyme composed of a regulatory (Bcy1) subunit dimer and two catalytic subunits (Tpk1, Tpk2 and Tpk3). PKA subunits are differentially expressed under certain stress conditions. In the present study we show that, although the mRNA levels of TPK1 are upregulated upon heat shock at 37℃, no change is detected in Tpk1 protein levels. The half-life of TPK1 mRNA increases and this mRNA condensates in cytoplasmic foci upon thermal stress. The resistance of TPK1 mRNA foci to cycloheximide-induced disassembly, together with the polysome profiling analysis suggest that TPK1 mRNA is impaired for entry into translation. TPK1 mRNA foci and TPK1 expression were also evaluated during thermotolerance. The crosstalk of cAMP-PKA pathway and cell wall integrity (CWI) signalling was also studied. Wsc3 sensor and other components of the CWI pathway are necessary for the upregulation of TPK1 mRNA upon heat shock conditions. The assembly in cytoplasmic foci upon thermal stress shows to be dependent of Wsc3. Finally, evidence of an increase in the abundance of Tpk1 in the PKA holoenzyme in response to heat shock is presented. The results indicate the existence of a mechanism that exclusively regulates Tpk1 subunit expression, which contributes to cAMP-PKA specificity and also suggest that a recurrent stress enhanced the fitness for the coming favorable conditions.

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The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae

Cited by 20 publications

References 54 publications

eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules

eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules

Control of Translation at the Initiation Phase During Glucose Starvation in Yeast

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

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