The role of pirfenidone in alkali burn rat cornea

Jiang, Nan; Ma, Mingyang; Li, Yunyan; Su, Ting; Zhou, Xuezhi; Ye, Lei; Yuan, Qing; Zhu, Pei‐Wen; Min, You‐Lan; Shi, Wen‐Qing; Xu, Xiaowei; Lv, Jiannan; Shao, Yi

doi:10.1016/j.intimp.2018.08.032

Cited by 19 publications

(21 citation statements)

References 17 publications

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“…Alkali injury of the cornea is one of the most devastating ophthalmic conditions [ 12 ] and can cause epithelial defects, acute inflammation, neovascularization, and decreased transparency of the cornea, resulting in blindness. The purpose of this study was to demonstrate the effect of a PPARβ/δ agonist using a rat corneal alkali burn model, which is widely used to study the mechanisms of treatments, acute inflammation, and neovascularization in the injured cornea [ 13 , 14 ]. In the present study, we used a synthetic PPARβ/δ-specific agonist, GW501516, that has been used in research of physiological and pathophysiological functions of PPARβ/δ in conditions including obesity, diabetes, dyslipidemia, and cardiovascular disease [ 15 , 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Peroxisome Proliferator-Activated Receptor Beta/Delta Agonist Suppresses Inflammation and Promotes Neovascularization

Tobita

Arima

Nakano

et al. 2020

IJMS

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The effects of peroxisome proliferator-activated receptor (PPAR)β/δ ophthalmic solution were investigated in a rat corneal alkali burn model. After alkali injury, GW501516 (PPARβ/δ agonist) or vehicle ophthalmic solution was topically instilled onto the rat’s cornea twice a day until day 7. Pathological findings were evaluated, and real-time reverse transcription polymerase chain reaction was performed. GW501516 strongly suppressed infiltration of neutrophils and pan-macrophages, and reduced the mRNA expression of interleukin-6, interleukin-1β, tumor necrosis factor alpha, and nuclear factor-kappa B. On the other hand, GW501516 promoted infiltration of M2 macrophages, infiltration of vascular endothelial cells associated with neovascularization in the wounded area, and expression of vascular endothelial growth factor A mRNA. However, 7-day administration of GW501516 did not promote neovascularization in uninjured normal corneas. Thus, the PPARβ/δ ligand suppressed inflammation and promoted neovascularization in the corneal wound healing process. These results will help to elucidate the role of PPARβ/δ in the field of ophthalmology.

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Section: Discussionmentioning

confidence: 99%

Peroxisome Proliferator-Activated Receptor Beta/Delta Agonist Suppresses Inflammation and Promotes Neovascularization

Tobita

Arima

Nakano

et al. 2020

IJMS

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“…Alkaline burn was reported to induce a dramatic up-regulation of IL-1β, IL-6, transforming growth factor β (TGF-β), CD34, CD31, VEGF etc. in the cornea (18,19,31). The cytokines could activate Src family kinase, which could in turn mediate the production of these cytokines (32).…”

Section: Discussionmentioning

confidence: 99%

“…The anti-tube formation effect of dasa-NLC was evaluated with a method reported before with modi cations (18). Brie y, Matrigel® Matrix was thawed at 4℃ overnight before being coated into a 96 well plate (50 µL/well) and the gel was solidi ed after being incubated in 37℃ for 30 min.…”

Section: Anti-tube Formation Assaymentioning

confidence: 99%

Dasatinib loaded nanostructured lipid carriers for effective treatment of corneal neovascularization

Zhang

et al. 2021

Biomater. Sci.

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“…However, in a subsequent study of corneal alkali burns in dogs, the beneficial effect of suberanilohydroxamic acid on corneal fibrosis was not confirmed [63]. Pirfenidone was also shown to enhance corneal wound healing not only by exerting antifibrotic effects but also by shortening the re-epithelialization time and exerting antiangiogenic effects [57,58,62].…”

Section: Antifibrotic Strategiesmentioning

confidence: 96%

“…Galectin-3 inhibitor [59] Ophthalmic (eye drops) EMP2 antibody blockade [60] Subconjunctival injection Humanized antibody H1L1 [61] Subconjunctival injection Antifibrotic strategies Suberanilohydroxamic acid [63] Topical Pirfenidone [57,58,62] Topical/eye drops/contact lens Re-epithelialization Gelatin/ascorbic acid cryogels [53] Surgical insertion tFNAs [55] Topical…”

Section: Antiangiogenic Strategiesmentioning

confidence: 99%

Recent Advances in Experimental Burn Models

Hao

Nourbakhsh

2021

Biology

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Experimental burn models are essential tools for simulating human burn injuries and exploring the consequences of burns or new treatment strategies. Unlike clinical studies, experimental models allow a direct comparison of different aspects of burns under controlled conditions and thereby provide relevant information on the molecular mechanisms of tissue damage and wound healing, as well as potential therapeutic targets. While most comparative burn studies are performed in animal models, a few human or humanized models have been successfully employed to study local events at the injury site. However, the consensus between animal and human studies regarding the cellular and molecular nature of systemic inflammatory response syndrome (SIRS), scarring, and neovascularization is limited. The many interspecies differences prohibit the outcomes of animal model studies from being fully translated into the human system. Thus, the development of more targeted, individualized treatments for burn injuries remains a major challenge in this field. This review focuses on the latest progress in experimental burn models achieved since 2016, and summarizes the outcomes regarding potential methodological improvements, assessments of molecular responses to injury, and therapeutic advances.

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The role of pirfenidone in alkali burn rat cornea

Cited by 19 publications

References 17 publications

Peroxisome Proliferator-Activated Receptor Beta/Delta Agonist Suppresses Inflammation and Promotes Neovascularization

Peroxisome Proliferator-Activated Receptor Beta/Delta Agonist Suppresses Inflammation and Promotes Neovascularization

Dasatinib loaded nanostructured lipid carriers for effective treatment of corneal neovascularization

Recent Advances in Experimental Burn Models

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